| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 555,570 | A→C | 37.0% | G159G (GGT→GGG) | moeB ← | molybdopterin synthase sulfurylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 555,570 | 0 | A | C | 37.0% | 22.3 / 37.5 | 46 | G159G (GGT→GGG) | moeB | molybdopterin synthase sulfurylase |
| Reads supporting (aligned to +/- strand): ref base A (15/14); new base C (5/12); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01 | |||||||||||
ACCAAACAAACGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGTACCTT > minE/555504‑555611 | aCCAAACAAACGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGAc < 1:293081/68‑1 (MQ=255)aCCAAACAAACGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGAc < 1:174719/68‑1 (MQ=255) caaacGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCAt > 1:1143387/1‑69 (MQ=255) caaacGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCAt > 1:2024660/1‑69 (MQ=255) caaacGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCAt > 1:1724050/1‑69 (MQ=255) cGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGa < 1:2935658/69‑1 (MQ=255) cGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGa < 1:1477269/69‑1 (MQ=255) gTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGc > 1:2772746/1‑68 (MQ=255) gTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGc > 1:2971881/1‑68 (MQ=255) gTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGc > 1:3085591/1‑68 (MQ=255) gTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGc > 1:3173403/1‑68 (MQ=255) gTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGc > 1:1764776/1‑68 (MQ=255) gTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGc > 1:3436078/1‑68 (MQ=255) gTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGc > 1:126520/1‑68 (MQ=255) tCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:3396860/69‑1 (MQ=255) tCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:739646/69‑1 (MQ=255) tCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:804407/69‑1 (MQ=255) tCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:1342001/69‑1 (MQ=255) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:3297646/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:3286226/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:77179/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:3074155/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:3055848/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:837120/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:1175198/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:2664772/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:2655228/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:256270/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:2519359/36‑1 (MQ=37) cGTCCTGATAAGTGAAGACCGTGATTTGCCCTTCCa < 1:2471984/36‑1 (MQ=37) gTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGTACCtt > 1:194711/1‑69 (MQ=255) gTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGTACCtt > 1:1367404/1‑69 (MQ=255) gTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGTACCtt > 1:696539/1‑69 (MQ=255) gTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGTACCtt > 1:3045963/1‑69 (MQ=255) cTGATAAGTAAAGACGGTGATTTGACCTTCCATACg > 1:1641317/1‑36 (MQ=255) aaGACCGTGATTTGCCCTTCCATACGAATTGCCGCGCCGGAAACCa > 1:1923185/1‑46 (MQ=39) aaGACCGTGATTTGCCCTTCCATACGAATTGCCGCGCCGGAAACCAg > 1:2341024/1‑47 (MQ=39) aaGACCGTGATTTGCCCTTCCATACGAATTGCCGCGCCGGAAACCAg > 1:2550149/1‑47 (MQ=39) aaGACCGTGATTTGCCCTTCCATACGAATTGCCGCGCCGGAAACCAg > 1:1393604/1‑47 (MQ=39) aaGACCGTGATTTGCCCTTCCATACGAATTGCCGCGCCGGAAACCAg > 1:1314686/1‑47 (MQ=39) tGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:2411290/39‑1 (MQ=255) tGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:2829814/39‑1 (MQ=255) tGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:361841/39‑1 (MQ=255) tGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:15043/39‑1 (MQ=255) tGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:752060/39‑1 (MQ=255) tGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGt < 1:894173/39‑1 (MQ=255) | ACCAAACAAACGGCTAAGGCAGCGATAGCACGGTTCACCGTCCTGATAAGTAAAGACGGTGATTTGACCTTCCATACGAATTGCCGCGCCGGAAACCAGCGGTACCTT > minE/555504‑555611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |