| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 556,511 | C→T | 42.0% | E257E (GAG→GAA) | moeA ← | molybdopterin biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 556,511 | 0 | C | T | 42.0% | 18.3 / 39.6 | 57 | E257E (GAG→GAA) | moeA | molybdopterin biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (10/23); new base T (8/16); total (18/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
ATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATA > minE/556449‑556571 | aTCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCAcc < 1:2690015/68‑1 (MQ=255)aTCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCAcc < 1:3302994/68‑1 (MQ=255) cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:698655/68‑1 (MQ=255) cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:2504372/68‑1 (MQ=255) cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:2129134/68‑1 (MQ=255) cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:1649141/68‑1 (MQ=255) cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:1856744/68‑1 (MQ=255) cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:419889/68‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1898531/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:3017527/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:2990743/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:3185615/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:274678/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:2104373/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:3400862/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:192328/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1735727/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:731475/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1414877/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:570353/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1481796/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1461643/69‑1 (MQ=255) cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:621683/69‑1 (MQ=255) cAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGa < 1:3038713/69‑1 (MQ=255) cAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGa < 1:1116514/69‑1 (MQ=255) cAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGa < 1:1213267/69‑1 (MQ=255) agtttCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1717554/65‑1 (MQ=255) aGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACAcc < 1:422246/67‑1 (MQ=255) aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTg < 1:1141994/69‑1 (MQ=255) aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTg < 1:2562687/69‑1 (MQ=255) aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTg < 1:1870375/69‑1 (MQ=255) tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:2137207/61‑1 (MQ=255) tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:384394/61‑1 (MQ=255) tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:20752/61‑1 (MQ=255) tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:1967463/61‑1 (MQ=255) tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:385313/61‑1 (MQ=255) ccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac < 1:2845622/67‑1 (MQ=255) cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:3206900/1‑58 (MQ=255) cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:918196/1‑58 (MQ=255) cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:275273/1‑58 (MQ=255) cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:261161/1‑58 (MQ=255) cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:3006625/1‑58 (MQ=255) cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:1801977/1‑58 (MQ=255) cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:1318788/1‑58 (MQ=255) cAAGAATCGTTTTGGCGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:134649/1‑58 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGctt > 1:1452156/1‑66 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:2465795/1‑69 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:2092400/1‑69 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:75684/1‑69 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:1748377/1‑69 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:1608289/1‑69 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:591483/1‑69 (MQ=255) aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:612180/1‑69 (MQ=255) gAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcaccac > 1:1165985/1‑58 (MQ=255) gAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGc > 1:2082795/1‑69 (MQ=255) tAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATa < 1:447892/69‑1 (MQ=255) tAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATa < 1:1850942/69‑1 (MQ=255) | ATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATA > minE/556449‑556571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |