| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 64,573 | G→A | 15.1% | I344I (ATC→ATT) | leuC ← | 3‑isopropylmalate isomerase subunit, dehydratase component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 64,573 | 0 | G | A | 15.1% | 125.0 / 9.6 | 72 | I344I (ATC→ATT) | leuC | 3‑isopropylmalate isomerase subunit, dehydratase component |
| Reads supporting (aligned to +/- strand): ref base G (19/42); new base A (9/2); total (28/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
TTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAG > minE/64508‑64638 | tttCGCCCTTTTGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa < 1:2787736/69‑1 (MQ=255)tttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa < 1:658168/69‑1 (MQ=255)tttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa < 1:764481/69‑1 (MQ=255)tttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa < 1:2448277/69‑1 (MQ=255)tttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa < 1:604970/69‑1 (MQ=255)tttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa < 1:260158/69‑1 (MQ=255) ttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGATCCGATa > 1:2381568/1‑68 (MQ=255) ttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa > 1:3426296/1‑68 (MQ=255) ttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa > 1:2032505/1‑68 (MQ=255) ttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa < 1:134610/68‑1 (MQ=255) ttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa > 1:2793775/1‑68 (MQ=255) ttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATa > 1:1132176/1‑68 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2664104/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:1023497/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2552601/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2513944/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2809646/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2876660/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2241994/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2996994/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:2089880/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:420232/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:1105205/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:1248787/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:1817512/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:1364279/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:1025600/68‑1 (MQ=255) gCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:1659348/68‑1 (MQ=255) cGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTc < 1:538892/67‑1 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATaa > 1:964297/1‑53 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAg > 1:1097119/1‑68 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:2756496/1‑69 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:261998/1‑69 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:1254817/1‑69 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:3127967/1‑69 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:211331/1‑69 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:423244/1‑69 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:281341/1‑69 (MQ=255) ctcCGCTGCCGCGCGTAAATCTTCAATGCGCCAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGc > 1:2845919/1‑69 (MQ=255) aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGaa < 1:2708499/69‑1 (MQ=255) aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGaa < 1:823226/69‑1 (MQ=255) aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGaa < 1:473721/69‑1 (MQ=255) aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGaa < 1:617921/69‑1 (MQ=255) gcgcGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGat < 1:2038585/59‑2 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:1053688/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:3089378/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:352623/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:207779/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:2823813/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:271187/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:1350160/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:2501226/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:2484070/56‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:677895/56‑1 (MQ=255) cgcgAGTTAGTACAGGAACCAATAAACACTTTGTc < 1:2105367/35‑1 (MQ=255) cgcgAGTTAGTACAGGAACCAATAAACACTTTGTc < 1:1921292/35‑1 (MQ=255) gcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:838394/55‑1 (MQ=255) tGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCACCGGAATACCCGGTTTCAGCCCCa < 1:1160215/69‑1 (MQ=255) tACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata < 1:256907/68‑1 (MQ=255) cAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg > 1:731690/1‑69 (MQ=255) ggAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg > 1:2101661/1‑67 (MQ=255) ggAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg > 1:1768964/1‑67 (MQ=255) ggAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg > 1:1141145/1‑67 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCa > 1:379482/1‑68 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCa > 1:1905648/1‑68 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCa > 1:1777248/1‑68 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg > 1:289588/1‑69 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg > 1:2493611/1‑69 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg > 1:1687546/1‑69 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg > 1:1470651/1‑69 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg > 1:985445/1‑69 (MQ=255) aCCAATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCACCa > 1:375345/1‑59 (MQ=255) | TTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAG > minE/64508‑64638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |