| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 561,090 | T→C | 12.7% | V41V (GTA→GTG) | ybjI ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 561,090 | 0 | T | C | 12.7% | 109.5 / 5.3 | 62 | V41V (GTA→GTG) | ybjI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (29/25); new base C (5/3); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAAAGAGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTT > minE/561050‑561158 | agaaagaGATCAACTGATAATATTGATTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCAttt < 1:1419605/69‑1 (MQ=255)agaaagaGATCAACTGATAATATTGATTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCAttt < 1:535100/69‑1 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt > 1:1180669/1‑69 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:1222210/69‑1 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:987380/69‑1 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:1278303/69‑1 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:3082425/69‑1 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:467667/69‑1 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:1599280/69‑1 (MQ=255) aagaGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:1593330/69‑1 (MQ=255) gagaTCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGa < 1:2411488/69‑1 (MQ=255) tCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCt > 1:839606/1‑53 (MQ=255) cAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTg < 1:2968938/69‑1 (MQ=255) cAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTg < 1:1899833/69‑1 (MQ=255) gATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCa < 1:724936/69‑1 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCAt > 1:1038079/1‑68 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:3156038/1‑69 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:1777281/1‑69 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:3089602/1‑69 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:608046/1‑69 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:1187064/1‑69 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:1899866/1‑69 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:2658106/1‑69 (MQ=255) taataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:2615524/1‑69 (MQ=255) aataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATaa > 1:333750/1‑69 (MQ=255) aataTTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATaa > 1:3042470/1‑69 (MQ=255) atatTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTg > 1:991144/1‑60 (MQ=255) atatTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCa < 1:2301322/65‑1 (MQ=255) atatTGATTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCAttt < 1:1247393/50‑1 (MQ=39) ttGATTCCCGCTGGCGACTACAAAGCGTATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCg > 1:2953193/1‑69 (MQ=255) ttGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:2128406/50‑1 (MQ=255) ttGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:2282175/50‑1 (MQ=255) ttGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:715017/50‑1 (MQ=255) ttGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:1252068/50‑1 (MQ=255) ttGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCg > 1:2393640/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:3028892/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:487390/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:1456582/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:2040785/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:2486461/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:1227762/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:2182620/1‑69 (MQ=255) gATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCt > 1:1498156/1‑69 (MQ=255) aTTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:192953/1‑61 (MQ=255) aTTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:1883768/1‑61 (MQ=255) aTTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:3219571/1‑61 (MQ=255) aTTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:1063884/1‑61 (MQ=255) aTTCCCGCTGGCGACCACAAAGCGAATGCCTTGTGCTTTCATTTGCTGATACTGAGCCATa > 1:222002/1‑61 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:927504/45‑1 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:992311/45‑1 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:1551029/45‑1 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:1879587/45‑1 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:233382/45‑1 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:2266229/45‑1 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCt < 1:2131965/45‑1 (MQ=255) tCCCGCTGGCGACTACAAAGCGAATTACTTGTGCTTTCATTTGCt < 1:3129043/45‑1 (MQ=255) cccGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAg > 1:1685895/1‑53 (MQ=255) cccGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTc > 1:375997/1‑66 (MQ=255) cccGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACg > 1:3140662/1‑69 (MQ=255) cccGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACg > 1:1941220/1‑69 (MQ=255) cccGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACg > 1:295652/1‑69 (MQ=255) tGGCGACTACAAAGCTAATTCCTTGTGCTTTCATTTGCt < 1:258302/39‑1 (MQ=255) tACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGtttt > 1:3417014/1‑69 (MQ=255) tACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGtttt > 1:1285573/1‑69 (MQ=255) tACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGtttt > 1:1962954/1‑69 (MQ=255) tACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGtttt > 1:2105275/1‑69 (MQ=255) tACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGtttt > 1:881731/1‑69 (MQ=255) tACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGtttt > 1:1013532/1‑69 (MQ=255) | AGAAAGAGATCAACTGATAATATTGATTCCCGCTGGCGACTACAAAGCGAATTCCTTGTGCTTTCATTTGCTGATACTGAGCCATAAACCGCTCACGGTTATAGGTTTT > minE/561050‑561158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |