| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 66,162 | C→T | 33.2% | A179A (GCG→GCA) | leuB ← | 3‑isopropylmalate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 66,162 | 0 | C | T | 33.2% | 93.2 / 58.7 | 111 | A179A (GCG→GCA) | leuB | 3‑isopropylmalate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (35/39); new base T (11/26); total (46/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GGATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCAT > minE/66096‑66226 | ggATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAAcgc < 1:2356134/69‑1 (MQ=255)ggATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAAcgc < 1:2372247/69‑1 (MQ=255)ggATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAAcgc < 1:3422198/69‑1 (MQ=255)ggATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAAcgc < 1:2763235/69‑1 (MQ=255)ggATTGCAGCACGTTGGC‑TTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAAcgc < 1:3157274/68‑1 (MQ=255)ggATTGCAGCACGTT‑GCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAAcgc < 1:1213102/68‑1 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:2507047/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:3149200/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:2026899/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:1978357/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:1834329/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:2405631/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:2940035/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:1489663/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:560337/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:791159/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:921590/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:2759942/1‑69 (MQ=255) ttGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:2458290/1‑69 (MQ=255) atGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGAt > 1:190591/2‑69 (MQ=255) tGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATg > 1:1485973/1‑69 (MQ=255) tGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATg > 1:2306278/1‑69 (MQ=255) tGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATg > 1:3311080/1‑69 (MQ=255) aCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGc > 1:374015/1‑68 (MQ=255) aCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGc > 1:1369042/1‑68 (MQ=255) aCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGc > 1:1939906/1‑68 (MQ=255) aCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGc > 1:1054587/1‑68 (MQ=255) aCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGc > 1:2185702/1‑68 (MQ=255) cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:3345695/69‑1 (MQ=255) cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:1178694/69‑1 (MQ=255) cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:1445065/69‑1 (MQ=255) cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:1039825/69‑1 (MQ=255) cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:2442644/69‑1 (MQ=255) tttGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:116922/67‑1 (MQ=255) gTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:67963/68‑1 (MQ=255) ttGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAAcgc < 1:1046071/56‑1 (MQ=255) gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:2949339/64‑1 (MQ=255) gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:2393511/64‑1 (MQ=255) gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:379505/64‑1 (MQ=255) gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:47786/64‑1 (MQ=255) gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa < 1:828546/64‑1 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGCTACGTTCGATCTCaaa > 1:1444324/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:3375904/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:62443/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:864462/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:948400/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:1109489/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:1982632/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:2277965/1‑69 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCACaaa > 1:3255493/1‑69 (MQ=255) cGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAAc < 1:2418108/68‑1 (MQ=255) tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt < 1:1618780/69‑1 (MQ=255) tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt < 1:1557086/69‑1 (MQ=255) tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt < 1:83242/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:600975/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:64075/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:637489/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:770307/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:1408959/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:1453605/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:816359/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:3131163/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2991424/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2450010/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGGGa < 1:936614/69‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2075128/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:862404/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:349424/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:1797209/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTAAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2980466/68‑1 (MQ=255) ttGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATac > 1:2469169/1‑69 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:1583695/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:596735/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:652317/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:800457/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:800489/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:1359437/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:910687/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:549807/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:453649/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:45355/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2636972/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2757195/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:180992/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:3371715/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:1889672/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2760293/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2954923/68‑1 (MQ=255) gCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2134094/67‑1 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTaca > 1:282892/1‑46 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCtttttta > 1:1201631/1‑67 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:2638739/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:1486213/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:1213701/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:834768/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:313144/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:3238677/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:3399370/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:349335/1‑69 (MQ=255) cAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCat < 1:1432849/69‑1 (MQ=255) | GGATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCAT > minE/66096‑66226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |