Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	564,523	T→A	25.6%	A126A (GCA→GCT)	ybjL ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	564,523	0	T	A	25.6%	60.0 / 20.8	43	A126A (GCA→GCT)	ybjL	predicted transporter
Reads supporting (aligned to +/- strand):  ref base T (5/27);  new base A (3/8);  total (8/35)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAT  >  minE/564457‑564585                                                                   |                                                               ttCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCt                                                                >  1:130314/1‑67 (MQ=255) ttCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTg                                                               >  1:1553852/1‑68 (MQ=255)    cATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGATCCTGCTaa                                                           >  1:1022009/1‑69 (MQ=255)    cATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTaa                                                           >  1:1855170/1‑69 (MQ=255)    cATGCCGGAATGACCCAGTGTATCGCCAGCACCGACCAGAACCTGTGTCGACGTCATAGAGCCTGCTaa                                                           >  1:2271637/1‑69 (MQ=255)      tGCCGGAATGACTCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:1455841/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:2258016/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:2023313/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:2019629/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:2440066/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:2456118/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:2588933/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:1687253/69‑1 (MQ=255)      tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa                                                         <  1:1510940/69‑1 (MQ=255)            aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:1742520/69‑1 (MQ=255)            aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:1596152/69‑1 (MQ=255)            aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:2671810/69‑1 (MQ=255)            aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:1462679/69‑1 (MQ=255)            aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:1394700/69‑1 (MQ=255)            aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:432741/69‑1 (MQ=255)            aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:1284598/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCTACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:2794829/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:2104079/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:2178893/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:3008212/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:1888696/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:2463886/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:3004335/69‑1 (MQ=255)                      tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:1472298/69‑1 (MQ=255)                        tATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa                                       <  1:2055597/69‑1 (MQ=255)                          tCGCCAGCACCGGCCAGAACCGGTGTCTACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa                                       <  1:3256655/67‑1 (MQ=255)                          tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa                                       <  1:316459/67‑1 (MQ=255)                          tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa                                       <  1:2971805/67‑1 (MQ=255)                          tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa                                       <  1:118006/67‑1 (MQ=255)                          tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa                                       <  1:1888114/67‑1 (MQ=255)                          tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa                                       <  1:1327437/67‑1 (MQ=255)                            gCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAatat                                    >  1:831783/1‑68 (MQ=255)                                       ccAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg                                                   <  1:262834/42‑1 (MQ=255)                                              cGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc                                         <  1:486175/45‑1 (MQ=255)                                                           aTAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCg    <  1:2935943/69‑1 (MQ=255)                                                           aTAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCg    <  1:2467908/69‑1 (MQ=255)                                                             agagCCAGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTa         >  1:1991591/1‑62 (MQ=255)                                                             agagCCAGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAt  >  1:2782037/1‑69 (MQ=255)                                                             agagCCAGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAt  >  1:1895600/1‑69 (MQ=255)                                                                   |                                                               TTCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAT  >  minE/564457‑564585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑