| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 566,936 | T→C | 78.2% | L271L (CTA→CTG) | poxB ← | pyruvate dehydrogenase (pyruvate oxidase), thiamin‑dependent, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 566,936 | 0 | T | C | 78.2% | 53.1 / 9.0 | 41 | L271L (CTA→CTG) | poxB | pyruvate dehydrogenase (pyruvate oxidase), thiamin‑dependent, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base T (7/2); new base C (8/24); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
AATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCC > minE/566876‑567004 | aaTCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTaa > 1:1111108/1‑69 (MQ=255)aaTCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTaa > 1:838329/1‑69 (MQ=255)aaTCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTaa > 1:1787030/1‑69 (MQ=255)aaTCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTaa > 1:2297283/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:986470/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:720410/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:446939/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:3219690/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:3035766/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:3021591/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:2827293/1‑69 (MQ=255) cggtcggGTAGAACGCGCGGTAGGGAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATgg > 1:1932861/1‑69 (MQ=255) tAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGaaa < 1:1987816/69‑1 (MQ=255) aGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGCTATGGAAACCTGACGAGAAg > 1:1823821/1‑68 (MQ=255) gggAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGcc > 1:3054387/1‑69 (MQ=255) gggAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGcc > 1:470475/1‑69 (MQ=255) gAAATTGCGTGCCTAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:3170452/62‑1 (MQ=255) gAAATTGCGTGCCGAGCTGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:811185/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATTGTATGGAAACCTGACgag < 1:2388992/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGTAAACCTGACgag < 1:777726/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:1322649/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:158949/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:3248004/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:3303434/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:618905/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:3163611/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:1123677/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:857454/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:861180/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:1062042/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:3163586/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:3146839/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:1691910/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:2855362/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:2767341/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:2612634/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:2556271/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:2538240/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACCTGACgag < 1:2204804/62‑1 (MQ=255) gAAATTGCGTGCCGAGCAGCACTAACGTATCGGCATTCATCATGGTATGGAAACATGACgag < 1:2189757/62‑1 (MQ=255) ttGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTaa < 1:2716202/69‑1 (MQ=255) tAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTcc < 1:2128776/69‑1 (MQ=255) tAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTcc < 1:1410407/69‑1 (MQ=255) tAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTcc < 1:654316/69‑1 (MQ=255) tAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTcc < 1:688510/69‑1 (MQ=255) | AATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCC > minE/566876‑567004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |