Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 66,174 G→A 52.3% I175I (ATC→ATT leuB ← 3‑isopropylmalate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE66,1740GA52.3% 3.2 / 96.5 107I175I (ATC→ATTleuB3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (12/39);  new base A (30/26);  total (42/65)
Fisher's exact test for biased strand distribution p-value = 1.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 107 total aligned reads displayed.

CGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCAT  >  minE/66107‑66226
                                                                   |                                                    
cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:1178694/69‑1 (MQ=255)
cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:2442644/69‑1 (MQ=255)
cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:3345695/69‑1 (MQ=255)
cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:1039825/69‑1 (MQ=255)
 tttGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:116922/67‑1 (MQ=255)
 gTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:67963/68‑1 (MQ=255)
     gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:47786/64‑1 (MQ=255)
     gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:2949339/64‑1 (MQ=255)
     gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:2393511/64‑1 (MQ=255)
     gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:828546/64‑1 (MQ=255)
     gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGa                                                     <  1:379505/64‑1 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGCTACGTTCGATCTCaaa                                     >  1:1444324/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:3375904/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:1982632/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:62443/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:2277965/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:2658571/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:3260352/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:864462/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:948400/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa                                     >  1:1109489/1‑69 (MQ=255)
                cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCACaaa                                     >  1:3255493/1‑69 (MQ=255)
                   cGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAAc                                   <  1:2418108/68‑1 (MQ=255)
                     tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt                                <  1:1557086/69‑1 (MQ=255)
                     tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt                                <  1:1618780/69‑1 (MQ=255)
                     tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt                                <  1:83242/69‑1 (MQ=255)
                     tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt                                <  1:1933725/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:3131163/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:2450010/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:600975/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:2991424/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:637489/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:64075/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:1408959/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:770307/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:1453605/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:816359/69‑1 (MQ=255)
                       aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGGGa                              <  1:936614/69‑1 (MQ=255)
                        cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:862404/68‑1 (MQ=255)
                        cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:1797209/68‑1 (MQ=255)
                        cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:1406797/68‑1 (MQ=255)
                        cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:349424/68‑1 (MQ=255)
                        cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:1457973/68‑1 (MQ=255)
                        cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:2075128/68‑1 (MQ=255)
                        cTTTGTGGCGACGCTTGCGAGCAGATTAAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:2980466/68‑1 (MQ=255)
                         tttGTGGCGACGCTGGCGAGCAGATTCAACCGCGATGCGGGCGATACGTGCGATCTCAAAACGGTGa                              <  1:414939/67‑1 (MQ=255)
                          ttGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATac                           >  1:2469169/1‑69 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:45355/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:800489/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:800457/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:453649/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:3371715/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:652317/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:549807/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:910687/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:587588/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:596735/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:98474/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:2757195/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:1359437/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:180992/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:2954923/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:2902651/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:1738205/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:2760293/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:2636972/68‑1 (MQ=255)
                              ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:1583695/68‑1 (MQ=255)
                               gCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:119376/67‑1 (MQ=255)
                               gCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:2134094/67‑1 (MQ=255)
                                    gCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc                        <  1:3400107/62‑1 (MQ=255)
                                        gCGAGCAGATTCAAACGCGATGCTGGCGATACGTTCGATCTCAAAACGGTGa                              <  1:2124011/52‑1 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGGTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCATTTTc    >  1:2356401/1‑68 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTaca                          >  1:282892/1‑46 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCtttttta   >  1:1201631/1‑67 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:313144/1‑69 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:1486213/1‑69 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:2638739/1‑69 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:834768/1‑69 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:1213701/1‑69 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:349335/1‑69 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:3399370/1‑69 (MQ=255)
                                                  tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa   >  1:3238677/1‑69 (MQ=255)
                                                   cAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCat  <  1:1432849/69‑1 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1658980/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:2247153/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1396307/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1217393/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1101977/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1667482/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1756481/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1809651/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:1842617/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:2330511/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:518641/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:2413313/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:2475870/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:2752492/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:336058/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa              >  1:3160936/1‑47 (MQ=255)
                                                             gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCa               >  1:269353/1‑46 (MQ=255)
                                                                   |                                                    
CGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCAT  >  minE/66107‑66226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: