| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 577,624 | T→C | 64.0% | R193R (CGT→CGC) | macB → | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 577,624 | 0 | T | C | 64.0% | 29.8 / 23.8 | 47 | R193R (CGT→CGC) | macB | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (8/9); new base C (19/11); total (27/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCA > minE/577571‑577671 | gACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGAtt > 1:2717557/1‑69 (MQ=255)gACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGAt > 1:1237645/1‑68 (MQ=255) aGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATc < 1:13267/69‑1 (MQ=255) cATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGt > 1:545064/1‑68 (MQ=255) cATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTc > 1:383587/1‑69 (MQ=255) cATTCTGGCGAAGAGGTGATGGAGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTc > 1:2498035/1‑69 (MQ=255) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCTGGCATACGGTGAtt > 1:212688/1‑61 (MQ=255) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGTGCATACGGTGAtt > 1:1823053/1‑61 (MQ=38) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:3089923/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:2540527/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:259383/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:2767699/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:2917859/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:3016162/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1014604/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:3374064/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:412384/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:564225/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:866229/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1009774/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1864516/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1630821/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1523351/1‑61 (MQ=39) ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTCCGCGATCGCGGGCAAACGGTGATTa > 1:2175977/1‑62 (MQ=255) ttCTCGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:341366/1‑61 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:3344942/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:942489/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:588242/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:1143941/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:11500/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:530443/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:525761/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:2926318/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:1447525/50‑1 (MQ=38) agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:263658/50‑1 (MQ=38) ggCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgc > 1:2930397/1‑69 (MQ=255) ggCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgc > 1:1348718/1‑69 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1359773/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1372404/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:408591/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1461172/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:2661846/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:617741/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:2025380/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:2288887/68‑1 (MQ=255) gATCCTGCATCAGCTGCGCGATCGCGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1306503/68‑1 (MQ=255) ccTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCa > 1:1354289/1‑69 (MQ=255) | GACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCA > minE/577571‑577671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |