| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 581,798 | T→C | 44.4% | T612T (ACT→ACC) | clpA → | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 581,798 | 0 | T | C | 44.4% | 24.1 / 103.5 | 124 | T612T (ACT→ACC) | clpA | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Reads supporting (aligned to +/- strand): ref base T (33/36); new base C (30/25); total (63/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 124 total aligned reads displayed.
AGCAGACTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCA > minE/581741‑581859 | aGCAGACTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATc < 1:1395385/69‑1 (MQ=255) gTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCtt < 1:1035535/68‑1 (MQ=255) gTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCtt < 1:2229355/68‑1 (MQ=255) tAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTa < 1:918588/68‑1 (MQ=255) tAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTa < 1:2753636/68‑1 (MQ=255) tAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTa < 1:1794739/68‑1 (MQ=255) aaaCGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCtt < 1:3298817/66‑1 (MQ=255) gtgCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg > 1:3294242/1‑69 (MQ=255) gtgCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg > 1:3119136/1‑69 (MQ=255) gtgCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg > 1:2895657/1‑69 (MQ=255) gtgCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg > 1:28031/1‑69 (MQ=255) gtgCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg > 1:2469342/1‑69 (MQ=255) gtgCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg > 1:1369212/1‑69 (MQ=255) cTGGTGATGCCCACCAACGCCGGGGTACGGGAAACTg > 1:2155386/1‑37 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTg > 1:1071749/1‑37 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTg > 1:2011223/1‑37 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTg > 1:1053796/1‑37 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTg > 1:2236669/1‑37 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTg > 1:2485825/1‑37 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTg > 1:420230/1‑37 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGAt < 1:674416/69‑1 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGAt < 1:659372/69‑1 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGAt < 1:2796444/69‑1 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGAt < 1:2965256/69‑1 (MQ=255) cTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGAt < 1:1906688/69‑1 (MQ=255) ggTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGAt < 1:202022/67‑1 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCtt < 1:1225352/48‑1 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCa > 1:3036559/1‑67 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:3381981/69‑1 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc > 1:2398979/1‑69 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc > 1:2219953/1‑69 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc > 1:1990209/1‑69 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc > 1:1883744/1‑69 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc > 1:1313720/1‑69 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:1326975/69‑1 (MQ=255) accaccAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc > 1:827506/1‑69 (MQ=255) ccactaACGCGGGGTTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:3295978/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc > 1:2552793/1‑68 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:1947632/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:330719/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:1138260/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:508367/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:2957405/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:2899323/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:1352358/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:2760456/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:1469850/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:1799122/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:193612/1‑69 (MQ=255) ccactaACGCGGGGGTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcg > 1:780280/1‑69 (MQ=255) ccaccaACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:1074978/68‑1 (MQ=255) ccaACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTa < 1:2044708/45‑1 (MQ=255) ccaACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:853260/65‑1 (MQ=255) ggggTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGaa < 1:3252351/69‑1 (MQ=255) ggggTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGaa < 1:1152389/69‑1 (MQ=255) gggTACGTGAAACCTAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:2626416/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATc > 1:2076625/1‑38 (MQ=38) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:1841462/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:2084157/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:1183935/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:1652518/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:1030763/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:3412027/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:3370239/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:884507/1‑66 (MQ=255) gggTACGTGAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATgg > 1:772875/1‑66 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:562924/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:2435395/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:1655730/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:137757/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:2763173/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:277199/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:118867/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:524094/53‑1 (MQ=255) tACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAcc < 1:635004/53‑1 (MQ=255) cGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAgaga > 1:1045743/1‑67 (MQ=255) tgAAACCGATCTCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:1799439/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGTAAGAGATCa < 1:682438/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:723792/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:690442/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:559402/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:2667248/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:31846/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:1116254/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:151428/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:1062810/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:1988932/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:2280815/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:2671235/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:2741981/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:2795861/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:3094084/67‑1 (MQ=255) tgAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:331988/67‑1 (MQ=255) agAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:1414360/67‑1 (MQ=255) gAAACCGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa < 1:2881001/67‑1 (MQ=255) aaaCTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa > 1:253716/1‑66 (MQ=255) aaaCTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa > 1:2903927/1‑66 (MQ=255) aaaCTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa > 1:2986293/1‑66 (MQ=255) aaaCTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa > 1:1736398/1‑66 (MQ=255) aaaCTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCa > 1:1326647/1‑66 (MQ=255) | AGCAGACTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCA > minE/581741‑581859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |