| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 581,912 | T→C | 36.1% | F650F (TTT→TTC) | clpA → | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 581,912 | 0 | T | C | 36.1% | 66.2 / 64.6 | 105 | F650F (TTT→TTC) | clpA | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Reads supporting (aligned to +/- strand): ref base T (31/36); new base C (27/11); total (58/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
CGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGC > minE/581845‑581979 | cgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTg < 1:8112/69‑1 (MQ=255)cgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGGCTCGACAACATTATCTGGTTTg < 1:787726/69‑1 (MQ=255) agagATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCt > 1:939250/1‑69 (MQ=255) agagATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCt > 1:1785079/1‑69 (MQ=255) gaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTc > 1:1470501/1‑69 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:154077/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:2049277/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:2924150/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:1840550/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:3160350/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:3391698/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:1675532/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:636038/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:1549714/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:740120/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:1504249/1‑68 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:905401/1‑68 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCg < 1:707641/68‑1 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCg < 1:528601/68‑1 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCg < 1:2479030/68‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatc < 1:2953861/47‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc > 1:2134283/1‑68 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc > 1:935780/1‑68 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc > 1:1980503/1‑68 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc > 1:3177153/1‑68 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc > 1:29066/1‑68 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATcc > 1:1273449/1‑68 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:2305010/69‑1 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:1142419/69‑1 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:1120593/69‑1 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:2316497/69‑1 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:1465896/69‑1 (MQ=255) ggAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:1469780/69‑1 (MQ=255) gAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggtg < 1:1257363/69‑1 (MQ=255) gAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggtg < 1:2971651/69‑1 (MQ=255) gAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:1216790/68‑1 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGatca > 1:1261885/1‑36 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGgtggtg > 1:2168123/1‑67 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGgtggtg > 1:2435798/1‑67 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGgtggtg > 1:1974224/1‑67 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGgtggtg > 1:3020611/1‑67 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGgtggtg > 1:3294145/1‑67 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGgtggtg > 1:3353400/1‑67 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:2540569/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:374459/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:243823/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:921097/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:97796/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:1709453/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:2231105/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:2226890/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:1728401/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:179256/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:1692083/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:1969942/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:1553086/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:1086994/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGtggtgg > 1:1520664/1‑68 (MQ=255) ccGTAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACAGACGTGATCCATCAGGtggtgg > 1:2305291/1‑68 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATaa < 1:2869545/69‑1 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATaa < 1:2173234/69‑1 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATaa < 1:2595846/69‑1 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATaa < 1:1950141/69‑1 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATaa < 1:181444/69‑1 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATaa < 1:77698/69‑1 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATaa < 1:2329556/69‑1 (MQ=255) tAACCGTCTCGACAACATTATCTGGTTCGATCATCTGTCAACCGACGTGATCCATCAGGT‑GTGGATaa < 1:470918/68‑1 (MQ=255) gTCTCTACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAAtt > 1:1745869/1‑67 (MQ=255) gTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTc > 1:1055043/1‑68 (MQ=255) gTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCa > 1:1225632/1‑69 (MQ=255) gTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCa > 1:3318375/1‑69 (MQ=255) gTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCa > 1:2565809/1‑69 (MQ=255) gTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCa > 1:2566727/1‑69 (MQ=255) ctcGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCAtc < 1:3429389/69‑1 (MQ=255) tcGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:1812564/52‑1 (MQ=255) tcGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCAtc < 1:767963/68‑1 (MQ=255) caacaTTATCTGGTTTGATCATCTCTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCAtcgtcg < 1:621885/68‑1 (MQ=255) aTTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTg < 1:539764/69‑1 (MQ=255) aTTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTg < 1:1513538/69‑1 (MQ=255) aTTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTg < 1:951459/69‑1 (MQ=255) aTTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTg < 1:3252969/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:2818138/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:1028324/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:2543686/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:2624570/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:114493/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:2630576/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:2673696/69‑1 (MQ=255) ttATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGc < 1:2141016/69‑1 (MQ=255) tATCTGGTTTGTTCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCAtcgtcg < 1:322283/62‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCa < 1:2866981/67‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCa < 1:3405849/67‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCa < 1:1297822/67‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCa < 1:1921851/67‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCa < 1:1088077/67‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCa < 1:261682/67‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCa < 1:3250736/67‑1 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGc > 1:2951577/1‑69 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGc > 1:3184283/1‑69 (MQ=255) ttGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGc > 1:536762/1‑69 (MQ=255) | CGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGC > minE/581845‑581979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |