Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 584,788 | T→A | 66.7% | I369I (ATA→ATT) | cydC ← | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 584,788 | 0 | T | A | 66.7% | 51.2 / 36.8 | 54 | I369I (ATA→ATT) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
Reads supporting (aligned to +/- strand): ref base T (15/3); new base A (28/8); total (43/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.33e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 |
GGTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTC > minE/584721‑584847 | ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:109590/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:1874045/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2369885/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2389412/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:1545235/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2659394/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2750141/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:1251072/69‑1 (MQ=255) ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:341830/69‑1 (MQ=255) gTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTAGCCCGAGAATCGCta < 1:3394160/68‑1 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGCATCGCTATATGTTcccc > 1:1633486/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:3415589/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:2737789/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:530842/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:243907/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:2423796/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:650173/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:88530/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1972102/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:2380198/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1781266/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1766109/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1615290/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1632693/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1636598/1‑69 (MQ=255) gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTccc > 1:51493/1‑68 (MQ=255) aGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:616924/52‑1 (MQ=255) aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:2023650/69‑1 (MQ=255) aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:1100124/69‑1 (MQ=255) aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:1286643/69‑1 (MQ=255) gATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATccc > 1:949302/1‑68 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAagag > 1:966876/1‑57 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:962986/1‑58 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1941431/1‑58 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1164450/1‑58 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1235738/1‑58 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:2131580/1‑58 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:3161298/1‑58 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1489610/1‑58 (MQ=255) tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:2865872/1‑58 (MQ=255) cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:1682363/1‑67 (MQ=255) cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:866304/1‑67 (MQ=255) cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:2075250/1‑67 (MQ=255) cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:2914413/1‑67 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2093628/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1845131/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1046937/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2151806/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:688586/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:113975/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:61813/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2515739/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1226913/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2588093/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2932022/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2743267/1‑57 (MQ=255) ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2899566/1‑57 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:482215/69‑1 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1299601/69‑1 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2464840/69‑1 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1539882/69‑1 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:324056/69‑1 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2716144/69‑1 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2036439/69‑1 (MQ=255) gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:220931/69‑1 (MQ=255) | GGTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTC > minE/584721‑584847 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |