| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 66,180 | T→C | 51.5% | E173E (GAA→GAG) | leuB ← | 3‑isopropylmalate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 66,180 | 0 | T | C | 51.5% | 4.8 / 95.8 | 108 | E173E (GAA→GAG) | leuB | 3‑isopropylmalate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (25/27); new base C (29/26); total (55/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGG > minE/66123‑66246 | cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGCTACGTTCGATCTCaaa > 1:1444324/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:1109489/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:948400/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:864462/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:3260352/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:1982632/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:62443/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCaaa > 1:3375904/1‑69 (MQ=255)cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCACaaa > 1:3255493/1‑69 (MQ=255) cGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAAc < 1:2418108/68‑1 (MQ=255) tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt < 1:1933725/69‑1 (MQ=255) tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt < 1:1618780/69‑1 (MQ=255) tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt < 1:1557086/69‑1 (MQ=255) tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGt < 1:83242/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2450010/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:600975/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2991424/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:3131163/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:637489/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:64075/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:770307/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:1408959/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:816359/69‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGGGa < 1:936614/69‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:1457973/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:862404/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:349424/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:1406797/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2075128/68‑1 (MQ=255) cTTTGTGGCGACGCTTGCGAGCAGATTAAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGa < 1:2980466/68‑1 (MQ=255) tttGTGGCGACGCTGGCGAGCAGATTCAACCGCGATGCGGGCGATACGTGCGATCTCAAAACGGTGa < 1:414939/67‑1 (MQ=255) ttGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATac > 1:2469169/1‑69 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2636972/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:98474/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2954923/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2902651/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2757195/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2760293/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2994437/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:3371715/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:453649/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:549807/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:587588/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:596735/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:652317/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:800457/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:800489/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:910687/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:180992/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:1738205/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2451764/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:1359437/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:1889672/68‑1 (MQ=255) ggCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:1583695/68‑1 (MQ=255) gCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:119376/67‑1 (MQ=255) gCGACGCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:2134094/67‑1 (MQ=255) gCTTGCGGGCAGATTCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACAcc < 1:3400107/62‑1 (MQ=255) gCGAGCAGATTCAAACGCGATGCTGGCGATACGTTCGATCTCAAAACGGTGa < 1:2124011/52‑1 (MQ=255) tCAAATGCGATGCGGGCAATACGGTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCATTTTc > 1:2356401/1‑68 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTaca > 1:282892/1‑46 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCtttttta > 1:1201631/1‑67 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:1486213/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:349335/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:3399370/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:313144/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:834768/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:1213701/1‑69 (MQ=255) tCAAATGCGATGCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCAAACGCTTTTTCa > 1:2638739/1‑69 (MQ=255) cAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCat < 1:1432849/69‑1 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1101977/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1658980/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1396307/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1667482/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1217393/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1756481/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1809651/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:1842617/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:2752492/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:2475870/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:2413313/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:336058/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:518641/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:3160936/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:2330511/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:2247153/1‑47 (MQ=255) gCGGGCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCa > 1:269353/1‑46 (MQ=255) gcggCAATACGCTCGATCTCAAAACGGTGGTACACCTCGGTATCaa > 1:362852/3‑46 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCttttt > 1:2544776/1‑47 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCata > 1:657383/1‑51 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCat > 1:1587120/1‑50 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:1543106/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:1295335/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:2307468/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:1573786/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:2123223/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:613645/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:3434037/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg > 1:1827104/1‑69 (MQ=255) aCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGACCGCTACCTTcgcg > 1:219132/1‑69 (MQ=255) cGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCgg > 1:592498/1‑69 (MQ=255) | CGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGG > minE/66123‑66246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |