| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 584,839 | A→G | 38.2% | S352S (TCT→TCC) | cydC ← | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 584,839 | 0 | A | G | 38.2% | 42.4 / 59.0 | 89 | S352S (TCT→TCC) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (36/19); new base G (18/16); total (54/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAAC > minE/584779‑584898 | gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2464840/69‑1 (MQ=255)gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:482215/69‑1 (MQ=255)gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2036439/69‑1 (MQ=255)gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2716144/69‑1 (MQ=255)gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1539882/69‑1 (MQ=255)gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:220931/69‑1 (MQ=255)gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1299601/69‑1 (MQ=255)gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:324056/69‑1 (MQ=255) gcaatatTTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:3115626/59‑1 (MQ=255) tGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGtgaa < 1:263834/68‑1 (MQ=255) tGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGtgaa < 1:1208402/68‑1 (MQ=255) ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:3163524/69‑1 (MQ=255) ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:2097561/69‑1 (MQ=255) ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:404235/69‑1 (MQ=255) ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:724596/69‑1 (MQ=255) ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:227732/69‑1 (MQ=255) ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:518216/69‑1 (MQ=255) ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:2039717/69‑1 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:2550959/1‑69 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:2550794/1‑69 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa < 1:61750/69‑1 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:895444/1‑69 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:3309389/1‑69 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa < 1:3265829/69‑1 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:941322/1‑69 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:3124747/1‑69 (MQ=255) tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa < 1:1737159/69‑1 (MQ=255) agaAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGa < 1:3238394/69‑1 (MQ=255) aTCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAAc > 1:493412/1‑68 (MQ=255) aTCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAAc > 1:3199331/1‑68 (MQ=255) aTCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAAc > 1:1857169/1‑68 (MQ=255) tGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1796532/1‑69 (MQ=255) tGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:222156/1‑69 (MQ=255) tGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:3274249/1‑69 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:3409127/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:450213/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:2924009/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:2528717/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:3028661/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1108280/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:2392354/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1431432/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1989579/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1631836/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1768354/1‑67 (MQ=255) ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGAAACGTCAGCGAAACGCGATCGGCAac > 1:3084928/1‑67 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1071878/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1220084/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:138500/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1411340/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1551348/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:408028/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2456968/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1698418/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:586930/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:487865/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2043214/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:526145/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2696670/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2102815/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2212053/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2396272/1‑63 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCa > 1:1954972/1‑62 (MQ=255) gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATAGGCaa > 1:3117574/1‑63 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:1013300/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:997635/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:351394/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:355390/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:714001/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:2069367/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:101955/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1505981/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1521637/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1610876/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1643479/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1741924/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1841536/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:336184/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:2745525/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:2761244/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:2776107/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:3009131/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:3111685/1‑60 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:3127842/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:3140723/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:3145250/60‑1 (MQ=255) ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:3307128/60‑1 (MQ=255) ttGAGATTGCTACGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:808642/60‑1 (MQ=255) ttGAGATTGCTACGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:826112/1‑60 (MQ=255) | GAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAAC > minE/584779‑584898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |