| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 584,917 | A→G | 55.9% | F326F (TTT→TTC) | cydC ← | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 584,917 | 0 | A | G | 55.9% | 27.7 / 79.6 | 102 | F326F (TTT→TTC) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (22/23); new base G (28/29); total (50/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
ATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCC > minE/584851‑584981 | aTAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGaaa < 1:1195908/69‑1 (MQ=255) tgaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTg < 1:498568/68‑1 (MQ=255) tgaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTg < 1:1435148/68‑1 (MQ=255) gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:1950797/1‑69 (MQ=255) gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:1695749/1‑69 (MQ=255) gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:1575694/1‑69 (MQ=255) gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:645326/1‑69 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:3039935/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2058359/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2027247/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2854218/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:419817/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2894927/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2759708/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2868622/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:944109/67‑1 (MQ=255) cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:1220645/67‑1 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:176744/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:1987394/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:1905352/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:427320/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:77616/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:49244/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:1285841/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:2878521/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:152905/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:1525206/1‑64 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGtt > 1:1532091/1‑63 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGtt > 1:1203064/1‑63 (MQ=255) gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGag > 1:859107/1‑68 (MQ=255) gTAATGTCAGCGAAACGCGATAGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:2467483/1‑64 (MQ=255) aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:1825163/1‑69 (MQ=255) aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:931577/1‑69 (MQ=255) aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:1500884/1‑69 (MQ=255) aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:334420/1‑69 (MQ=255) aCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:642648/1‑68 (MQ=255) ctgCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:848727/52‑1 (MQ=255) aGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTaa > 1:2309371/1‑68 (MQ=255) aGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTaa > 1:2386915/1‑68 (MQ=255) aGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTaa > 1:1689968/1‑68 (MQ=255) aGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTaa > 1:37985/1‑68 (MQ=255) aGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTaa > 1:425729/1‑68 (MQ=255) aGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTaa > 1:1149133/1‑68 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2732936/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2850113/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:1095508/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2986330/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:3191267/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:636638/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:790720/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:953526/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:1391922/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2521649/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2108167/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2220452/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:1312758/69‑1 (MQ=255) aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2320704/69‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2728868/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:775721/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2762838/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:71539/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCaga < 1:199720/69‑1 (MQ=255) aCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCaga < 1:132196/68‑1 (MQ=255) aTCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGAtacg < 1:2837226/69‑1 (MQ=255) aTCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGAtacg < 1:2886885/69‑1 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGt > 1:3074663/1‑41 (MQ=38) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:3385605/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:501320/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:34068/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1573157/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1957572/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1712305/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1646098/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2263063/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2417322/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1283573/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2605155/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2668414/1‑69 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGTTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3095659/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:740767/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:922357/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:120003/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2652395/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:558691/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:1015901/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:181623/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2024600/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3331875/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3118903/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3088804/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2872876/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2323975/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2777570/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2559149/69‑1 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACtttc > 1:2105606/1‑65 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGcc > 1:2105191/1‑68 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:1594560/1‑67 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:1447063/1‑67 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:1355932/1‑67 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:2735116/1‑67 (MQ=255) | ATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCC > minE/584851‑584981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |