| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 584,947 | G→A | 46.8% | D316D (GAC→GAT) | cydC ← | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 584,947 | 0 | G | A | 46.8% | 13.0 / 79.5 | 94 | D316D (GAC→GAT) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (24/26); new base A (26/18); total (50/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
AAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCC > minE/584883‑585013 | aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2850113/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:636638/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2108167/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:3191267/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2220452/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2320704/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2521649/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2986330/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:1391922/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:790720/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2732936/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:1312758/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:953526/69‑1 (MQ=255)aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:1095508/69‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2728868/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:2762838/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:775721/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCag < 1:71539/68‑1 (MQ=255) aaCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCaga < 1:199720/69‑1 (MQ=255) aCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCaga < 1:132196/68‑1 (MQ=255) aTCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGAtacg < 1:2837226/69‑1 (MQ=255) aTCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGAtacg < 1:2886885/69‑1 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1957572/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:501320/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1573157/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2417322/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:34068/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1283573/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1712305/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:3385605/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2605155/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:1646098/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2668414/1‑69 (MQ=255) cGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgta > 1:2263063/1‑69 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGTTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3095659/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2559149/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:181623/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2973010/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2652395/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:922357/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2777570/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:120003/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:1015901/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2872876/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3088804/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2323975/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:558691/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3118903/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:3331875/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:740767/69‑1 (MQ=255) aGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2024600/69‑1 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACtttc > 1:2105606/1‑65 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGcc > 1:2105191/1‑68 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:1447063/1‑67 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:1594560/1‑67 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:1355932/1‑67 (MQ=255) aGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGc > 1:2735116/1‑67 (MQ=255) gTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc < 1:2761266/50‑1 (MQ=255) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:1090744/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2919907/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:78441/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2989821/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2899658/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:1987505/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2084554/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2150907/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:1681681/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:1590842/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2557881/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2568208/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2596846/1‑37 (MQ=25) gACTTCCGGTTTTTGATCCGTTAAATCAGTGAtccgt > 1:2618648/1‑37 (MQ=25) tttttGATCCGTTAAGTCAGATATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAcc < 1:1334439/69‑1 (MQ=255) ttttGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAcc < 1:3091760/68‑1 (MQ=255) ttttGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAcc < 1:1387319/68‑1 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACt < 1:2984505/68‑1 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:1714620/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:1885132/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:1958362/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:968100/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:526077/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:998555/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:3421050/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:3344868/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:2211364/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:2964187/1‑69 (MQ=255) ccGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg > 1:2817261/1‑69 (MQ=255) aaGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc > 1:1975113/1‑69 (MQ=255) aaGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc > 1:2165285/1‑69 (MQ=255) aaGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc > 1:3309495/1‑69 (MQ=255) aaGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc > 1:3252085/1‑69 (MQ=255) aaGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc > 1:2310151/1‑69 (MQ=255) aaGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc > 1:1105474/1‑69 (MQ=255) aaGTCAGAGAAACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc > 1:2402443/1‑69 (MQ=255) | AAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCC > minE/584883‑585013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |