| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 585,997 | A→T | 37.0% | V555V (GTT→GTA) | cydD ← | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 585,997 | 0 | A | T | 37.0% | 69.4 / 75.2 | 89 | V555V (GTT→GTA) | cydD | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (27/29); new base T (18/15); total (45/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGGGCCACCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTG > minE/585932‑586059 | aaTGGGGCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:48847/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:2328049/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:2091397/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:1942871/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:193236/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:1894418/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:2129693/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:2669593/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:2697749/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:493621/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:1588206/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:2889982/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:3047904/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:1522732/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:603908/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:3097572/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:3145816/1‑68 (MQ=255)aaTGGGCCGCCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCTGACCATCCTGCATTAc > 1:2465614/1‑68 (MQ=255) tGGGCCACCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCa > 1:458935/1‑69 (MQ=255) tGGGCCACCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCa > 1:2483220/1‑69 (MQ=255) tGGGCCACCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCa > 1:3211149/1‑69 (MQ=255) tGGGCCACCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCa > 1:3183625/1‑69 (MQ=255) aGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACAt > 1:2703576/1‑68 (MQ=255) cacaCTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCa < 1:426582/69‑1 (MQ=255) cacaCTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCa < 1:2149091/69‑1 (MQ=255) cacaCTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCa < 1:1807029/69‑1 (MQ=255) cacaCTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCa < 1:850117/69‑1 (MQ=255) cacaCTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCa < 1:1107010/69‑1 (MQ=255) cacaCTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCa < 1:2160879/69‑1 (MQ=255) acacTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCa < 1:3343583/68‑1 (MQ=255) cacTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATccaa < 1:2260333/67‑3 (MQ=255) tCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAg < 1:1329655/68‑1 (MQ=255) tCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGa < 1:291656/69‑1 (MQ=255) tAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGAtcttct > 1:525651/1‑69 (MQ=255) tAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGAtcttct > 1:262611/1‑69 (MQ=255) tAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGAtcttct < 1:4103/69‑1 (MQ=255) tAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGAtcttct < 1:2241409/69‑1 (MQ=255) tAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGAtcttct > 1:617126/1‑69 (MQ=255) tAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGAtcttct > 1:1769293/1‑69 (MQ=255) tAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGAtcttc > 1:2740864/1‑68 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:2326978/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:862042/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:331706/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:805937/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:803052/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt > 1:700610/1‑69 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt > 1:1508284/1‑69 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:1528722/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt > 1:1574930/1‑69 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:1587616/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:1600252/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:594172/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:2267019/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:2653324/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:1781287/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:563233/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:1787670/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt > 1:2546377/1‑69 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:2541508/69‑1 (MQ=255) cTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt > 1:251216/1‑69 (MQ=255) ttGCTCAATTTTCCGGCGATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACtggt < 1:1740590/68‑1 (MQ=255) taatCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGAcc > 1:469805/1‑68 (MQ=255) aatCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGa > 1:3331269/1‑46 (MQ=255) aatCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCa > 1:2534428/1‑68 (MQ=255) aatCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCa > 1:2000678/1‑68 (MQ=255) aatCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCa > 1:1341974/1‑68 (MQ=255) aatCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCa > 1:1662179/1‑68 (MQ=255) aatCCGGCCACCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCa > 1:899866/1‑68 (MQ=255) gCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGt < 1:2175436/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTATCTGGTGGGTGACCATTAACGTTGTCTg < 1:995805/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCtt < 1:2699330/69‑2 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1440130/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1034359/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1076358/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1216411/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1220606/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:708869/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1445357/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1657892/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:2577803/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:2826833/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:2861560/69‑1 (MQ=255) cTGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:3053068/69‑1 (MQ=255) cTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACt > 1:3161930/1‑44 (MQ=255) cTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTggtgggt > 1:2373168/1‑51 (MQ=255) cTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg > 1:1372855/1‑69 (MQ=255) cTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg > 1:127710/1‑69 (MQ=255) cTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg > 1:814837/1‑69 (MQ=255) tGCATTACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTg < 1:1144558/68‑1 (MQ=255) | AATGGGCCACCAGCCACACTTAATTCCGCGTAACGTCCTTGCTCAATAATCCGGCCATCCTGCATAACCCAAATGACATCCCAGTCAGCAAGATCTTCTAACTGGTGGGTGACCATTAACGTTGTCTG > minE/585932‑586059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |