| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 586,705 | G→A | 65.9% | G319G (GGC→GGT) | cydD ← | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 586,705 | 0 | G | A | 65.9% | 53.4 / 44.0 | 79 | G319G (GGC→GGT) | cydD | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (19/8); new base A (28/24); total (47/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01 | |||||||||||
CCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAA > minE/586641‑586762 | ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:2949387/1‑67 (MQ=255)ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:981469/1‑67 (MQ=255)ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:2230740/1‑67 (MQ=255)ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:1357107/1‑67 (MQ=255)ccGCCTCACCACGTTG‑GGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAAc > 1:2304959/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGAt > 1:1492334/1‑64 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:1647431/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:2608468/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:2854522/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:298850/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:2050419/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:2014008/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:30083/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:3118006/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:3231661/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:1071013/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:346822/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:1123559/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:521668/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:577131/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:1127607/69‑1 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:3319821/1‑69 (MQ=255) aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:891135/1‑69 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCTAGATCGCGTAATg < 1:3191617/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:641896/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2685717/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2754871/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2940688/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:842066/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2958824/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:681730/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2990887/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:3000196/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:30899/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1081647/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:3383940/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:331982/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2553191/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1674325/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1528427/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1786315/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1416464/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2222194/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2367632/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1294858/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:121523/69‑1 (MQ=255) ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2488531/69‑1 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATgg > 1:2214079/1‑67 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg > 1:2395320/1‑66 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1342572/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1384445/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1192614/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1429158/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1514330/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:970383/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:3308650/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:3150955/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:3202086/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:256210/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2751521/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2814456/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1744073/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2483380/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2908742/1‑68 (MQ=255) tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1886626/1‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2616810/4‑67 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:3159692/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:923529/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:2487988/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:385224/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:3009865/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:3360979/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:3116177/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:1642233/4‑68 (MQ=255) caaaCTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCt > 1:1653295/4‑68 (MQ=255) cTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGaaa > 1:1600022/1‑69 (MQ=255) cTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGaaa > 1:3144158/1‑69 (MQ=255) cTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGaaa > 1:1181419/1‑69 (MQ=255) cTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACAGAGATCGCGTAATGGCTg > 1:2043667/1‑65 (MQ=255) | CCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAA > minE/586641‑586762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |