| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 591,494 | A→G | 23.1% | P524P (CCA→CCG) | ftsK → | DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 591,494 | 0 | A | G | 23.1% | 109.0 / 27.6 | 86 | P524P (CCA→CCG) | ftsK | DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Reads supporting (aligned to +/- strand): ref base A (27/39); new base G (10/10); total (37/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGT > minE/591437‑591538 | tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:992537/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:979117/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:93249/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3388159/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3333613/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3205927/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3109781/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:2921323/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:2821666/1‑69 (MQ=255)tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:1331677/1‑69 (MQ=255) aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1008427/1‑69 (MQ=255) aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:878847/1‑69 (MQ=255) aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:3160256/1‑69 (MQ=255) aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1891916/1‑69 (MQ=255) aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1799185/1‑69 (MQ=255) aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1274909/1‑69 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1636853/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:993666/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:840089/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:581053/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1143927/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:3251482/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:3191618/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:3150189/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:2676211/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:2437615/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1437090/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1785000/1‑68 (MQ=255) aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1484125/1‑68 (MQ=255) ttGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAg < 1:1140757/69‑1 (MQ=255) ttGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAg < 1:92276/69‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAagaaccagaac > 1:253529/1‑36 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGAAAGCAGCTTCTGtt < 1:1924750/68‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGtt < 1:3140996/68‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCg > 1:1120354/1‑38 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:1626873/1‑67 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1902401/67‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:912975/67‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:530406/67‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:1758481/1‑67 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:1763028/1‑67 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1278271/67‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:2061715/67‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:2062451/67‑1 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:2608435/1‑67 (MQ=255) aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:2440580/67‑1 (MQ=255) aaCCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTgc > 1:2599332/1‑67 (MQ=255) aaCCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTgc > 1:2952874/1‑67 (MQ=255) aaCCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTgc > 1:2994567/1‑67 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:2601153/48‑1 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:513010/48‑1 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1786686/48‑1 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1229149/48‑1 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:554182/48‑1 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:681733/48‑1 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1890828/48‑1 (MQ=255) cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1987217/48‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:527110/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:1005421/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2762533/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:3169108/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2831100/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2834891/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:1217871/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACATTCTGTTGCAGCAGt < 1:2132138/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGTACCTTCTGTTGCAGCAGt < 1:3373899/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGTACCTTCTGTTGCAGCAGt < 1:1707451/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:3102832/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:1143801/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:854648/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2273379/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2934683/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:675821/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:618675/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:3101848/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:541268/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:515680/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2823543/69‑1 (MQ=255) gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:121076/69‑1 (MQ=255) aTTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGTACCTTCTGTTGCAGCAGt < 1:2442144/68‑1 (MQ=255) aTTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2601626/68‑1 (MQ=255) tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:3223745/45‑1 (MQ=255) tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1681830/45‑1 (MQ=255) tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:644275/45‑1 (MQ=255) tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:374778/45‑1 (MQ=255) tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1186634/45‑1 (MQ=255) tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:3294034/45‑1 (MQ=255) accataaccGATCTAATCTTCGCTGAAAGCACATTCTGTTGCAGCAGt < 1:27510/48‑1 (MQ=255) | TGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGT > minE/591437‑591538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |