| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 593,687 | T→C | 41.8% | G1255G (GGT→GGC) | ftsK → | DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 593,687 | 0 | T | C | 41.8% | 27.6 / 63.1 | 86 | G1255G (GGT→GGC) | ftsK | DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Reads supporting (aligned to +/- strand): ref base T (36/14); new base C (18/18); total (54/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTC > minE/593621‑593750 | cTGGAAATCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc > 1:2139329/1‑69 (MQ=255)cTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGgtggtg > 1:1367604/1‑68 (MQ=255)cTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc > 1:2900940/1‑69 (MQ=255)cTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc > 1:2697625/1‑69 (MQ=255)cTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc > 1:482203/1‑69 (MQ=255)cTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc > 1:765233/1‑69 (MQ=255)cTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc > 1:2494718/1‑69 (MQ=255)cTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc > 1:1395902/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGATGGTGGTGCggg > 1:3092876/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:2490657/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:3334696/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:1753946/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:1595759/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:1457678/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:2393318/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:3009888/1‑69 (MQ=255) gAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCggg > 1:739999/1‑69 (MQ=255) aaGCGCGTGGTCGCCCACTGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:3416103/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:1118903/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:360298/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:1068362/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:467809/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:2262812/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:3145067/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:16639/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:728158/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:2604721/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:1487927/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:1473721/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:1367202/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTgggcg > 1:1406333/1‑67 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:2640226/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:2753364/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:2539448/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:2812044/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:400548/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:1966494/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:971188/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:903404/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:1263174/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:1841722/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:780889/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:1273479/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:1389078/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:140743/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:661280/67‑1 (MQ=255) aaGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:1523884/67‑1 (MQ=255) aaGCGCGTGGGCGCCCACAGTATGTTGATGGCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTggg < 1:224141/67‑1 (MQ=255) ggTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGAt < 1:1865064/69‑1 (MQ=255) gTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGAt > 1:2245536/1‑68 (MQ=255) gTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATg > 1:2696398/1‑69 (MQ=255) gTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATg > 1:2018888/1‑69 (MQ=255) aGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaag < 1:1704452/69‑1 (MQ=255) aGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaag < 1:1784796/69‑1 (MQ=255) aGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaag < 1:2203765/69‑1 (MQ=255) aGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaag < 1:2039137/69‑1 (MQ=255) aGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaag < 1:2782489/69‑1 (MQ=255) aGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaag < 1:1913924/69‑1 (MQ=255) gTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaaga < 1:633093/69‑1 (MQ=255) gTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaaga < 1:2420283/69‑1 (MQ=255) gTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaaga < 1:1184226/69‑1 (MQ=255) gTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaaga < 1:1112506/69‑1 (MQ=255) ttGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:3270948/1‑69 (MQ=255) ttGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:980917/1‑69 (MQ=255) ttGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTg > 1:1048500/1‑68 (MQ=255) ggCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTGGGCGGTTTCGATGGCGCTGAAGAACTGGATcc > 1:1418665/1‑68 (MQ=255) ggCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTGGGCGGTTTCGATGGCGCTGAAGAACTGGATCCg > 1:826381/1‑69 (MQ=255) ggCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTGGGCGGTTTCGATGGCGCTGAAGAACTGGATCCg > 1:3082089/1‑69 (MQ=255) ggCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTGGGCGGTTTCGATGGCGCTGAAGAACTGGATCCg > 1:787323/1‑69 (MQ=255) ggCATCACCTCCGACAGTGAAAGCGAAGGTGGCGTGGGCGGTTTCGATGGCGCTGAAGAACTGGATCCg > 1:291914/1‑69 (MQ=255) aCCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTc > 1:2171024/1‑69 (MQ=255) aCCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTc > 1:1999302/1‑69 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:1804752/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:110786/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg < 1:1282902/46‑1 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:137620/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:1395200/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:1509894/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:1816107/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:1928556/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:3344883/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:2304648/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:2305110/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:2376527/1‑46 (MQ=255) gaaagcgaaGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg > 1:1021099/1‑46 (MQ=255) aGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGt < 1:520262/69‑1 (MQ=255) aGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGt < 1:1268282/69‑1 (MQ=255) ggtggtGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGt < 1:967047/68‑1 (MQ=255) ggtggtGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTc < 1:2434851/69‑1 (MQ=255) gtggtgCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGt < 1:1414537/67‑1 (MQ=255) | CTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTC > minE/593621‑593750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |