| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 594,043 | C→T | 54.1% | intergenic (+131/‑25) | ftsK → / → lolA | DNA‑binding membrane protein required for chromosome resolution and partitioning/chaperone for lipoproteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 594,043 | 0 | C | T | 54.1% | 0.0 / 28.4 | 37 | intergenic (+131/‑25) | ftsK/lolA | DNA‑binding membrane protein required for chromosome resolution and partitioning/chaperone for lipoproteins |
| Reads supporting (aligned to +/- strand): ref base C (15/2); new base T (7/13); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTAC > minE/593983‑594101 | ttttCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGa < 1:178643/69‑1 (MQ=255) ttcttcTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGAc > 1:2636493/1‑68 (MQ=255) ttcttcTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGAc > 1:1754437/1‑68 (MQ=255) tGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATTGGGAGTGACGTAATTTGAGGaataat > 1:508285/1‑68 (MQ=255) tGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATTGGGAGTGACGTAATTTGAGGAATAatg > 1:3077595/1‑69 (MQ=255) tGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATTGGGAGTGACGTAATTTGAGGAATAatg > 1:465866/1‑69 (MQ=255) tGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATTGGGAGTGACGTAATTTGAGGAATAatg > 1:1963053/1‑69 (MQ=255) tGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATTGGGAGTGACGTAATTTGAGGAATAatg > 1:1955656/1‑69 (MQ=255) tGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATTGGGAGTGACGTAATTTGAGGAATAatg > 1:195000/1‑69 (MQ=255) tGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATTGGGAGTGACGTAATTTGAGGAATAatg > 1:159524/1‑69 (MQ=255) aTAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:2533854/68‑1 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:3181455/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:269940/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:3107655/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:3062062/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:2581561/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:2528029/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:1697624/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:1661439/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:1135693/1‑69 (MQ=255) gagGGAACTCCCGATCGGGAGTGACGTAAGTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGc > 1:2410834/1‑69 (MQ=255) aaCTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTAc > 1:2341231/1‑69 (MQ=255) aaCTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTAc > 1:146547/1‑69 (MQ=255) aaCTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTAc > 1:825381/1‑69 (MQ=255) gATTGGGAGTGACGTAATTTGAGTAATAATGATGAAAAAAATTGcc < 1:2290217/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:384296/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:938569/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:3054378/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:2946273/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:2857865/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:2731407/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:2581893/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:2260633/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:2092957/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:1967170/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:1325758/46‑1 (MQ=255) gATTGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGcc < 1:1292978/46‑1 (MQ=255) | TTTTCTTCTTTCCTCAAGCTGATTATTAGCCTGGAATAGAGAGTAGAGGGAACTCCCGATCGGGAGTGACGTAATTTGAGGAATAATGATGAAAAAAATTGCCATCACCTGTGCATTAC > minE/593983‑594101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |