| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 67,361 | G→A | 27.4% | G303G (GGC→GGT) | leuA ← | 2‑isopropylmalate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 67,361 | 0 | G | A | 27.4% | 76.6 / 25.7 | 69 | G303G (GGC→GGT) | leuA | 2‑isopropylmalate synthase |
| Reads supporting (aligned to +/- strand): ref base G (19/31); new base A (8/11); total (27/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGT > minE/67300‑67419 | tttGGTTCAGCCCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATcc > 1:1316520/1‑68 (MQ=255) ttCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg < 1:2300113/68‑1 (MQ=255) ttCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg < 1:385648/68‑1 (MQ=255) ttCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg < 1:1903406/68‑1 (MQ=255) aCCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATAc < 1:2104952/68‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATATCggagg < 1:441469/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:1769173/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2360249/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2475742/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2255160/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2077608/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2572708/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2845526/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:1938951/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2957173/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:3244746/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:1494236/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:390579/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:444822/69‑1 (MQ=255) aataTTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:981394/66‑1 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:2671777/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:3031831/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:281081/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:267223/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:261652/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:3292122/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:907419/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:1126634/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:1135237/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:2057547/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:1256654/1‑56 (MQ=255) gATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg > 1:2030217/1‑56 (MQ=255) aTTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCt < 1:3394464/37‑1 (MQ=255) aTTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCt < 1:2989737/37‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:2407626/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:1333375/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:3208649/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:3177830/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:1727194/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:1988453/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:2595626/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:2002410/68‑1 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:2282342/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:1151966/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:2920317/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:1714183/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:113701/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:1146017/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:40713/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:3189514/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac < 1:1084642/69‑1 (MQ=255) gTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac < 1:3207759/69‑1 (MQ=255) ttCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaacaa > 1:1658292/1‑68 (MQ=255) ttCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAAt > 1:2942100/1‑69 (MQ=255) ttCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAAt > 1:44899/1‑69 (MQ=255) ttCGCGGTTTTTCAGCACGCAATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaacaa > 1:3133332/1‑68 (MQ=255) ttttCAGTACACTATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2807979/44‑1 (MQ=25) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2340772/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:791427/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:1993936/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:3428923/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2666574/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:3147708/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:3120243/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2795113/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2852562/44‑1 (MQ=37) ttttCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCtttgt > 1:2769140/1‑69 (MQ=255) ttttCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCtttgt > 1:2848113/1‑69 (MQ=255) tCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2901649/41‑1 (MQ=37) | TTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGT > minE/67300‑67419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |