| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 597,804 | C→T | 52.7% | A49A (GCC→GCT) | dmsA → | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 597,804 | 0 | C | T | 52.7% | 1.3 / 60.7 | 71 | A49A (GCC→GCT) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (9/24); new base T (21/16); total (30/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACATTACCTTTTAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACTGTGGTAGT > minE/597762‑597864 | aaCATTACCTTTTAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGt > 1:3113800/1‑69 (MQ=255)aaCATTACCTTTTAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGt < 1:2533997/69‑1 (MQ=255)aaCATTACCTTTTAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGt < 1:2423035/69‑1 (MQ=255)aaCATTACCTTTTAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGt > 1:2372025/1‑69 (MQ=255) aCCTTTTAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTg < 1:1676050/69‑1 (MQ=255) ttttAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTgg < 1:1756379/67‑1 (MQ=255) ttAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAgcg > 1:629780/1‑69 (MQ=255) ttAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAgcg > 1:157226/1‑69 (MQ=255) gTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:958353/68‑1 (MQ=255) gTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:48902/68‑1 (MQ=255) gTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:2146763/68‑1 (MQ=255) gTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:2034830/68‑1 (MQ=255) gTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:118616/68‑1 (MQ=255) gTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:122513/68‑1 (MQ=255) tCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:255233/67‑1 (MQ=255) cGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:1026078/66‑1 (MQ=255) cGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:1251893/66‑1 (MQ=255) ggATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGTTTATCTGGAGCGCCTGTa < 1:2830454/69‑1 (MQ=255) ggATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTg > 1:274796/1‑67 (MQ=255) ggATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTg > 1:2517543/1‑67 (MQ=255) ggATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTg > 1:500003/1‑67 (MQ=255) ggATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTa < 1:856925/69‑1 (MQ=255) aTTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGcc < 1:204630/63‑1 (MQ=255) aTTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTAc < 1:567/68‑1 (MQ=255) tGCGCACGCTGTCGATAGCGCAATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTAc < 1:1918899/66‑1 (MQ=255) cACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAAc < 1:1052975/69‑1 (MQ=255) cACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAAc < 1:2825391/69‑1 (MQ=255) cACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAAc < 1:874172/69‑1 (MQ=255) cACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAAc < 1:286662/69‑1 (MQ=255) cACGCTGTCGATAGCGCCAATCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAAc < 1:319721/69‑1 (MQ=255) gTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTAc < 1:1169745/56‑1 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa > 1:2584807/1‑55 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:1023158/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:517976/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:835115/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:2848216/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:2862849/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:3037386/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:3048703/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:356400/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:476366/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:263555/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:268347/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:1582571/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:234549/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:1585875/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:2339738/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:2208690/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:148881/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:1707602/1‑64 (MQ=255) cGATAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATATGGAGCGCCTGTACAGTTAACtgt > 1:3104685/1‑64 (MQ=255) cGATAGCGCTAGTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACtgt > 1:1501181/1‑64 (MQ=255) gATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACTGTGGTAGt > 1:3406264/1‑69 (MQ=255) gATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACTGTGGTAGt > 1:1191187/1‑69 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTTCa < 1:1994596/52‑1 (MQ=39) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:2392480/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:3283008/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:943805/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:1160874/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:2167991/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:1758901/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:558164/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:532730/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:503039/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:2000794/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:1237932/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:2048840/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:445416/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACa < 1:2114090/52‑1 (MQ=255) tAGCGCTATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCCCCTGTACa < 1:301011/52‑1 (MQ=255) gcCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTAc < 1:855469/47‑1 (MQ=255) | AACATTACCTTTTAGTCGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGGAGCGCCTGTACAGTTAACTGTGGTAGT > minE/597762‑597864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |