| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 600,640 | T→C | 40.7% | F176F (TTT→TTC) | dmsB → | dimethyl sulfoxide reductase, anaerobic, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 600,640 | 0 | T | C | 40.7% | 28.7 / 37.0 | 54 | F176F (TTT→TTC) | dmsB | dimethyl sulfoxide reductase, anaerobic, subunit B |
| Reads supporting (aligned to +/- strand): ref base T (11/21); new base C (12/10); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-02 | |||||||||||
CTATCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCT > minE/600573‑600704 | cTATCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGTGCTCACTTTa < 1:2569101/69‑1 (MQ=255)cTATCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTa < 1:751555/69‑1 (MQ=255)cTATCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTa < 1:2316222/69‑1 (MQ=255)cTATCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTa < 1:2313159/69‑1 (MQ=255)cTATCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTa < 1:1805159/69‑1 (MQ=255)cTATCGACGAGCTGCGCAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTa < 1:3235764/69‑1 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:3254663/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:2789216/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:2545236/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:2388409/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:2174998/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:3165755/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:329078/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:1789396/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:3338724/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:838285/1‑69 (MQ=255) tCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCa > 1:539101/1‑69 (MQ=255) gCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGaa < 1:1642728/69‑1 (MQ=255) gCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGaa < 1:2944504/69‑1 (MQ=255) ggCGATCTGGTGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAAccc < 1:1022455/69‑1 (MQ=255) ggCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAAccc < 1:2261339/69‑1 (MQ=255) ggCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAAccc < 1:518891/69‑1 (MQ=255) ggCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAAccc < 1:1615599/69‑1 (MQ=255) ggCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAAccc < 1:1361765/69‑1 (MQ=255) ggCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAAccc < 1:2581829/69‑1 (MQ=255) ggCGATATGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAAccc < 1:483429/69‑1 (MQ=255) ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:2946783/64‑1 (MQ=255) ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:579287/64‑1 (MQ=255) ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:3090806/64‑1 (MQ=255) ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:946247/64‑1 (MQ=255) ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:2565478/64‑1 (MQ=255) ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:2068549/64‑1 (MQ=255) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1132918/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:2754564/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:611161/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:2294519/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1612339/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1694529/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1998667/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1986411/69‑1 (MQ=14) gttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1869732/68‑1 (MQ=14) gttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:2023196/68‑1 (MQ=14) ccTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGc > 1:1415494/2‑68 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGc > 1:2614088/1‑68 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGc > 1:926649/1‑68 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:1998413/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:2634767/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:405885/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:1441293/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:628226/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:125046/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:1235497/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:2741310/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCAGCCCAACCGGGGATACCACTGGCt > 1:2951432/1‑69 (MQ=17) | CTATCGACGAGCTGCGTAAAAAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCT > minE/600573‑600704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |