| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 67,373 | T→G | 36.6% | I299I (ATA→ATC) | leuA ← | 2‑isopropylmalate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 67,373 | 0 | T | G | 36.6% | 26.0 / 43.8 | 52 | I299I (ATA→ATC) | leuA | 2‑isopropylmalate synthase |
| Reads supporting (aligned to +/- strand): ref base T (6/27); new base G (8/11); total (14/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
ACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCA > minE/67310‑67434 | aCCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATAc < 1:2104952/68‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATATCggagg < 1:441469/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2845526/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:444822/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2957173/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:1494236/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2572708/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:390579/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:3244746/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2475742/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:1769173/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:1938951/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2360249/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2255160/69‑1 (MQ=255) tAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:2077608/69‑1 (MQ=255) aataTTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg < 1:981394/66‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:2002410/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:3208649/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:3177830/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:1988453/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:2407626/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:1727194/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:2595626/68‑1 (MQ=255) tAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGcc < 1:1333375/68‑1 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:3189514/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:40713/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:2920317/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:113701/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:1146017/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:1151966/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:1714183/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac > 1:2282342/1‑69 (MQ=255) gTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac < 1:1084642/69‑1 (MQ=255) gTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaac < 1:3207759/69‑1 (MQ=255) ttCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaacaa > 1:1658292/1‑68 (MQ=255) ttCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAAt > 1:44899/1‑69 (MQ=255) ttCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAAt > 1:2942100/1‑69 (MQ=255) ttCGCGGTTTTTCAGCACGCAATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCcaacaa > 1:3133332/1‑68 (MQ=255) ttttCAGTACACTATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2807979/44‑1 (MQ=25) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2852562/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:791427/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2795113/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:3428923/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:1993936/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2340772/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:3147708/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:3120243/44‑1 (MQ=37) ttttCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2666574/44‑1 (MQ=37) ttttCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCtttgt > 1:2769140/1‑69 (MQ=255) ttttCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCtttgt > 1:2848113/1‑69 (MQ=255) tCAGTACACCATCCTGGTGGATACCGGAGGAGTGTGCGAAt < 1:2901649/41‑1 (MQ=37) ccTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCa < 1:1707229/69‑1 (MQ=255) | ACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCA > minE/67310‑67434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |