| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 600,670 | T→C | 53.5% | N186N (AAT→AAC) | dmsB → | dimethyl sulfoxide reductase, anaerobic, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 600,670 | 0 | T | C | 53.5% | ‑3.5 / 33.0 | 43 | N186N (AAT→AAC) | dmsB | dimethyl sulfoxide reductase, anaerobic, subunit B |
| Reads supporting (aligned to +/- strand): ref base T (2/18); new base C (12/11); total (14/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-02 | |||||||||||
GGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAACCCGAAGGAGGTGTGAG > minE/600610‑600731 | ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:946247/64‑1 (MQ=255)ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:3090806/64‑1 (MQ=255)ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:2946783/64‑1 (MQ=255)ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:2565478/64‑1 (MQ=255)ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:579287/64‑1 (MQ=255)ggcCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGcc < 1:2068549/64‑1 (MQ=255) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1132918/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1612339/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1694529/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1986411/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:2754564/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1998667/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:611161/69‑1 (MQ=14) cgttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:2294519/69‑1 (MQ=14) gttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:1869732/68‑1 (MQ=14) gttgcCGCGTGCGCACTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:2023196/68‑1 (MQ=14) ccTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGc > 1:1415494/2‑68 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGc > 1:926649/1‑68 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGc > 1:2614088/1‑68 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:405885/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:628226/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:1235497/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:125046/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:2634767/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:2741310/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:1998413/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCGGGGATACCACTGGCt > 1:1441293/1‑69 (MQ=17) aCTTCACCAAACCGAATATTGTGATCAAACCTAACGCCAATAGCAGCCCAACCGGGGATACCACTGGCt > 1:2951432/1‑69 (MQ=17) ccGAATATTGTGATCAAACCTAACGCCAATAGCCGCCCAACCg < 1:3091324/43‑1 (MQ=17) ccGAATATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAAc > 1:2320700/1‑69 (MQ=255) ccGAATATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAAc > 1:1363304/1‑69 (MQ=255) atTGTTATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:361086/39‑1 (MQ=255) atTGTGATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:1015799/39‑1 (MQ=255) atTGTGATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:2140658/39‑1 (MQ=255) atTGTGATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:70222/39‑1 (MQ=255) ttGTGATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:541133/38‑1 (MQ=255) ttGTGATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:854502/38‑1 (MQ=255) ttGTGATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:1249028/38‑1 (MQ=255) ttGTGATCAAACCCAATGCCAATAGCCGCCCGACCggg < 1:933354/38‑1 (MQ=255) aTCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAACCCGAAGGAGgt < 1:2159385/68‑1 (MQ=255) tCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAACCCGAAGGAGgt < 1:3029519/67‑1 (MQ=255) aaaCCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAACCCGAAGGAGGTGTga < 1:1057555/69‑1 (MQ=255) aCCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAACCCGAAGGAGGTGTgag < 1:2222015/68‑1 (MQ=255) | GGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAACCCGAAGGAGGTGTGAG > minE/600610‑600731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |