| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 601,323 | G→A | 100% | V198M (GTG→ATG) | dmsC → | dimethyl sulfoxide reductase, anaerobic, subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 601,323 | 0 | G | A | 82.0% | 46.7 / 3.7 | 33 | V198M (GTG→ATG) | dmsC | dimethyl sulfoxide reductase, anaerobic, subunit C |
| Reads supporting (aligned to +/- strand): ref base G (1/5); new base A (0/27); total (1/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGGCTGGGCGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCT > minE/601260‑601387 | gATGTCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGAGTCGTGATGTCa < 1:2700124/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:1834069/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2695973/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2663611/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2767115/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2437765/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2424441/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2296463/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2248369/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2242669/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2894875/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2174528/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2110302/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2915357/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:2954829/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:1669738/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:3300349/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:1533111/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:465020/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:1285133/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:1200161/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:1171902/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:702713/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTCATGTCa < 1:441740/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCCATTTCTGTTCTGGCACTGGTAGTGAGGGGCGTGATGTCa < 1:2915567/69‑1 (MQ=255)gATGGCTGGGCGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCa > 1:1298157/1‑69 (MQ=255) tgctgcCAGCCATTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:3005822/50‑1 (MQ=255) ctgcCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCt < 1:2195345/68‑1 (MQ=255) ctgcCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCt < 1:576384/68‑1 (MQ=255) gccagccaTTTCTGTTCTGGCACTGGTAGTGAGTGGCGTGATGTCa < 1:1118055/46‑1 (MQ=255) gcCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCt < 1:2528714/66‑1 (MQ=255) gcCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCt < 1:1888017/66‑1 (MQ=255) gcCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCt < 1:297145/66‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:2195230/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1792384/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:2945402/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1749194/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1736818/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1622306/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1490974/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1441404/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1316421/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:707509/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:737421/68‑1 (MQ=255) gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:832513/68‑1 (MQ=255) tggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCt < 1:1114290/67‑1 (MQ=255) | GATGGCTGGGCGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCT > minE/601260‑601387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |