| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,600,816 | 2 bp→AT | 100% | coding (1086‑1087/1425 nt) | murP → | fused predicted enzyme IIBC components of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,600,816 | 0 | G | A | 85.9% | 18.8 / ‑2.7 | 14 | A362A (GCG→GCA) | murP | fused predicted enzyme IIBC components of PTS |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (5/7); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 1,600,817 | 0 | C | T | 85.9% | 18.8 / ‑2.8 | 14 | L363L (CTA→TTA) | murP | fused predicted enzyme IIBC components of PTS |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (5/7); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTAC > minE/1600756‑1600882 || aGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCa < 1:2012981/68‑1 (MQ=255) aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc < 1:2132299/46‑1 (MQ=255) aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc < 1:2384637/46‑1 (MQ=255) aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc < 1:2966939/46‑1 (MQ=255) aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc < 1:298809/46‑1 (MQ=255) aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc < 1:628919/46‑1 (MQ=255) aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc < 1:906550/46‑1 (MQ=255) aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc < 1:919597/46‑1 (MQ=255) ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAAcc > 1:2996069/1‑68 (MQ=255) ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg > 1:2625751/1‑69 (MQ=255) ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg > 1:3322573/1‑69 (MQ=255) ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg > 1:382316/1‑69 (MQ=255) ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg > 1:489666/1‑69 (MQ=255) acaGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGa < 1:2061341/69‑1 (MQ=255) gcgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTAc > 1:947300/1‑69 (MQ=255) || AGGCGCGGGCCAGGTGGGCGCGGCGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTAC > minE/1600756‑1600882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |