New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE = 490191116 (1.520)4 (0.050) 4/98 NT 3.4% coding (571/720 nt) pnuC predicted nicotinamide mononucleotide transporter
?minE = 490213 114 (1.530)coding (593/720 nt) pnuC predicted nicotinamide mononucleotide transporter
Rejected: Frequency below/above cutoff threshold.

ATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/490140‑490191
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aATAACGACGCTAATCACGTTAATAATCACCCACAACAGCCAGTTTTCCACATACTTACGCGTCATCAGAA  <  minE/490213‑490144
                                                                                                                          
ATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTAATAACGACGCTAATCACGT                                                     >  1:336705/1‑71
            ACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTAATAACGACGCTAATCA                                                        >  1:2513671/1‑56
              GCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTA                                                                        >  1:2100696/1‑38
                   AGTATGTGGAAAACTGGCTGTTGTGGGTGATTAATAACGACGCTAATCACGTT                                                    <  1:3567329/53‑1
                     TATGTGGAAAACTGGCTGTTGTGGGTGATTAATAACGACGCTAATCACGTTA                                                   >  1:959590/1‑52
                                                   AATAACGACGCTAATCACGTTAATAATCACCCACAACAGCCAGTTTTCCACATACTTACGCGTCATCAGAA  >  1:2718002/1‑71
                                                                                                                          
ATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/490140‑490191
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aATAACGACGCTAATCACGTTAATAATCACCCACAACAGCCAGTTTTCCACATACTTACGCGTCATCAGAA  <  minE/490213‑490144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.