New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE 2217293 =93 (1.220)3 (0.040) 3/90 NT 3.8% coding (1322/1941 nt) yhdA conserved inner membrane protein
?minE 2217312 = 69 (1.010)coding (1303/1941 nt) yhdA conserved inner membrane protein
Rejected: Frequency below/above cutoff threshold.

AAAACCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/2217367‑2217293
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaagaTGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG  >  minE/2217312‑2217381
                                                                                                                                                 
AAAACCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATG                                                                            <  1:2759121/71‑1
  AACCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAA                                                                          <  1:4022256/71‑1
  AACCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAA                                                                          <  1:3452773/71‑1
  AACCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATG                                                                            >  1:1466059/1‑69
   ACCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAAG                                                                         <  1:3968099/71‑1
    CCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAAG                                                                         <  1:4104800/70‑1
     CGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAAGA                                                                        <  1:1170758/70‑1
                      GGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAAGA                                                                        >  1:882691/1‑53
                              TCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAA                                                                          >  1:919744/1‑43
                                                    CGCATCTTCGATGGTAATGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAC                                       <  1:2170832/56‑1
                                                    CGCATCTTCGATGGTAATGAAGATGCGGCACATGAGTTCGCGATGA                                                 <  1:3240699/46‑1
                                                              ATGGTAATGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTT               <  1:2467834/70‑1
                                                                      GAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGC      >  1:3745123/1‑71
                                                                      GAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGC      <  1:665614/71‑1
                                                                       AAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCG     >  1:4220807/1‑71
                                                                        AGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGC    <  1:1451044/71‑1
                                                                          ATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG  <  1:2643715/71‑1
                                                                          ATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG  <  1:1357707/71‑1
                                                                          ATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGG                    <  1:1948291/53‑1
                                                                                                                                                 
AAAACCGGCGGTTACTCGCGAAGGTCAGGTTCATCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  minE/2217367‑2217293
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaagaTGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG  >  minE/2217312‑2217381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.