New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE = 263683970 (0.920)3 (0.040) 3/94 NT 4.5% coding (562/888 nt) ugpA glycerol‑3‑phosphate transporter subunit
?minE = 2636860 62 (0.870)coding (583/888 nt) ugpA glycerol‑3‑phosphate transporter subunit
Rejected: Frequency below/above cutoff threshold.

GCCTCAGTATGGAAGCAAATCAGCTACAACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/2636767‑2636839
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTG  <  minE/2636860‑2636793
                                                                                                                                             
GCCTCAGTATGGAAGCAAATCAGCTACAACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTC                                                                        >  1:1301786/1‑71
 CCTCAGTATGGAAGCAAATCAGCTACAACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCG                                                                       <  1:4404064/71‑1
 CCTCAGTATGGAAGCAAATCAGCTACAACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCG                                                                       <  1:2613198/71‑1
  CTCAGTGTGGAAGCAAATCAGCTACAACTTTCTGTTCTTCTATGCCGCGCTGCAATCCATCCCCCGTTCGT                                                                      >  1:3352587/1‑71
  CTCAGTATGGAAGCAAATCAGCTACAACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGT                                                                      >  1:2576671/1‑71
              GCAAATCAGCTACAACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTCGATGGCTGCGG                                                          >  1:685956/1‑71
                           AACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTCGATGGCTGCGGCTTCGATCAACGA                                             <  1:3910170/71‑1
                                CCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTCGATGGCTGCGGCTTCGATCAACGAACG                                          <  1:2926470/69‑1
                                   GTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTC                                                                        <  1:4273687/36‑1
                                    TTCTTCTATGCCGCGTTGCAATCCATTCCCCGTTCGT                                                                      <  1:947097/37‑1
                                                                      CGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAG              <  1:1603691/59‑1
                                                                      CGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAG              <  1:1229445/59‑1
                                                                      CGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAA               <  1:4236662/58‑1
                                                                      CGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAA               <  1:581054/58‑1
                                                                       GTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTG  >  1:3925552/1‑70
                                                                       GTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCA                    >  1:4280673/1‑52
                                                                       GTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCA                    >  1:4398955/1‑52
                                                                       GTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGAT                                >  1:1174382/1‑40
                                                                        TCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTG  >  1:3001616/1‑69
                                                                        TCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAA            <  1:3312571/59‑1
                                                                        TCGATGGCTGAGGCTTCGATCAAAGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTG  >  1:1626029/1‑69
                                                                                                                                             
GCCTCAGTATGGAAGCAAATCAGCTACAACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/2636767‑2636839
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTG  <  minE/2636860‑2636793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.