New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE = 264743799 (1.300)4 (0.060) 3/90 NT 4.3% intergenic (+117/‑156) asd/glgB aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme
?minE = 2647463 88 (1.280)intergenic (+143/‑130) asd/glgB aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme
Rejected: Frequency below/above cutoff threshold.

TGCGTAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/2647363‑2647437
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑taaggCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAAT  <  minE/2647463‑2647397
                                                                                                                                              
TGCGTAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTT                                                                         <  1:469560/71‑1
TGCGTAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTT                                                                         <  1:4032762/71‑1
  CGTAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTT                                                                         >  1:3345471/1‑69
    TAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGG                                                                     >  1:3064976/1‑71
    TAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAA                                                                       <  1:1970380/69‑1
    TAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAA                                                                       <  1:331341/69‑1
       TACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTT                                                                         <  1:4631750/64‑1
       TACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTT                                                                         <  1:377205/64‑1
           GGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTT                                                                         >  1:2873254/1‑60
                             TCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGG                                                                     >  1:3836048/1‑46
                              CATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTA                                                                        <  1:2341742/42‑1
                                TGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAG                                                                      <  1:1795010/42‑1
                                       CCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGCTGTGAATACTCATG                                                      <  1:1511415/51‑1
                                        CGGGAGTTAAATAGAGCATTGGCTATTCTGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAA                                 <  1:3102177/71‑1
                                        CGGGAGTTAAATAGAGCATTGGCTATTCTGTAAGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAA                                 <  1:3096595/71‑1
                                              TTAAATAGAGCATTGGCTATTCTTTAAGGCTGTGAATACTC                                                         <  1:790900/41‑1
                                                                       AAGGCTGTGAATACTCATGTATTCAGCAACCCTTAAAGAATAGCAAATGCTCTATTTAAATACCGGTAA    <  1:1141051/69‑1
                                                                        AGGCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAAT  <  1:330088/70‑1
                                                                          GCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCC                            <  1:1904509/42‑1
                                                                                                                                              
TGCGTAATACAGGAGTAAGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  minE/2647363‑2647437
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑taaggCTGTGAATACTCATGTATTCAGCCACCCTTAAAGAATAGCCAATGCTCTATTTAACTCCCGGTAAAT  <  minE/2647463‑2647397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.