| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 69,740 | T→C | 5.6% | H211H (CAT→CAC) | leuO → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 69,740 | 0 | T | C | 5.6% | 344.1 / 9.1 | 125 | H211H (CAT→CAC) | leuO | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (81/37); new base C (3/4); total (84/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGC > minE/69672‑69808 | aGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATgcgg > 1:2488507/1‑71 (MQ=255) gCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATg > 1:1793926/1‑63 (MQ=255) cAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGttt > 1:3344951/1‑71 (MQ=255) cAAAAATCATCCAACAATTAAGGGCCCGTTACAGAAACATGATGTTTATAACGAACAACATGCGGCGGttt > 1:3784291/1‑71 (MQ=255) aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTc > 1:4488115/1‑71 (MQ=255) aaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGc > 1:1775884/1‑71 (MQ=255) aaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCt > 1:4295338/1‑71 (MQ=255) atcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTc > 1:1062576/1‑71 (MQ=255) tcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTc > 1:795400/1‑70 (MQ=255) ccAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGAt > 1:4254742/1‑69 (MQ=255) ccAACAATTAAAGGCCCGTTACTGAAGCATGATGTTTATAACGAACAACACGCTGCGGTTTCGCTCGATCg > 1:2381194/1‑71 (MQ=255) caacaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAA‑AACATGCGGCGGTTTCGCTCGATCGt < 1:1902533/70‑1 (MQ=255) aacaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGt < 1:3566638/70‑1 (MQ=255) acaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGttt > 1:2399486/1‑58 (MQ=255) caaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTc < 1:4469750/71‑1 (MQ=255) aTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGcggcgg > 1:1414466/1‑52 (MQ=255) tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGttt < 1:1456648/53‑1 (MQ=255) tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGt > 1:3331273/1‑71 (MQ=255) aaGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGAt > 1:746375/1‑60 (MQ=255) ggCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCAtt < 1:2539078/71‑1 (MQ=255) gCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCAtt > 1:1640004/1‑70 (MQ=255) cccGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCAtt > 1:4463165/1‑69 (MQ=255) ccGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAg < 1:4406109/71‑1 (MQ=255) cGTTACTGAAGCATGATGTTTATAACGAACAACACGCTGCGGTTTCGCTCGa < 1:2049148/52‑1 (MQ=255) cGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGt > 1:4478833/1‑71 (MQ=255) cGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGt > 1:4473834/1‑71 (MQ=255) gTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc < 1:3437887/71‑1 (MQ=255) ttACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCg < 1:2105767/49‑1 (MQ=255) ttACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGAt < 1:3550919/51‑1 (MQ=255) ttACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCa > 1:872009/1‑63 (MQ=255) ttACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc > 1:2090041/1‑70 (MQ=255) tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCg < 1:638831/52‑1 (MQ=255) aCTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGAt > 1:2470359/1‑49 (MQ=255) aCTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGttt > 1:3928807/1‑54 (MQ=255) aCTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc > 1:2662963/1‑71 (MQ=255) aCTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc > 1:654094/1‑71 (MQ=255) cTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc < 1:1679532/71‑1 (MQ=255) aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCaa > 1:2664809/1‑66 (MQ=255) aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCa < 1:345377/65‑1 (MQ=255) aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTg > 1:104966/1‑71 (MQ=255) aaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg > 1:628105/1‑71 (MQ=255) aaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg > 1:1878817/1‑71 (MQ=255) aaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg < 1:4337896/71‑1 (MQ=255) aaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg > 1:3104292/1‑71 (MQ=255) aaCATGATGTTTATAACGAACAACATGCGGAGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg > 1:2831424/1‑71 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg > 1:1084358/1‑70 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg > 1:4607000/1‑70 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGt < 1:4512402/71‑1 (MQ=255) cATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTa < 1:3987668/71‑1 (MQ=255) atgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTAt < 1:3976746/71‑1 (MQ=255) atgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTAt > 1:2768913/1‑71 (MQ=255) atgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTAt < 1:4594559/71‑1 (MQ=255) tgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTAt > 1:2059727/1‑70 (MQ=255) tgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTAt < 1:3389766/70‑1 (MQ=255) atgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTg < 1:2775656/64‑1 (MQ=255) tgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGaca > 1:3679146/1‑71 (MQ=255) tttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCaa < 1:4101495/56‑1 (MQ=255) tttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGacac < 1:1370445/70‑1 (MQ=255) ttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCa < 1:2527285/46‑1 (MQ=255) ttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTg > 1:284745/1‑60 (MQ=255) ttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATg > 1:3101348/1‑65 (MQ=255) tataACGAACAACATGCGGCGGTTTCGCTCGATCg > 1:2127032/1‑35 (MQ=255) tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg < 1:4367208/70‑1 (MQ=255) tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg < 1:2694618/70‑1 (MQ=255) tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg > 1:2237957/1‑70 (MQ=255) tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg > 1:2781671/1‑70 (MQ=255) tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg < 1:1347899/70‑1 (MQ=255) tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg > 1:3251334/1‑70 (MQ=255) taACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg < 1:1301218/58‑1 (MQ=255) taACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg > 1:2748101/1‑68 (MQ=255) taACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg > 1:3489404/1‑68 (MQ=255) aaCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCAttt < 1:1155575/45‑1 (MQ=255) aaCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc < 1:1086152/52‑1 (MQ=255) aCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAg > 1:684857/1‑69 (MQ=255) aCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGAt < 1:2417037/71‑1 (MQ=255) aCGAACAACACGCTGCGGTTTCGCTCGATCGTTTCGCATCATTTAGTCAAACCTGGTATGACACGGTAGa < 1:2978657/70‑1 (MQ=255) aCGAACAACACGCTGCGGTTTCGCTCGATCGTTTCGCATCATTTAGTCAAACCTGGTATGACACGGTAGa < 1:4233732/70‑1 (MQ=255) cGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTa > 1:1543620/1‑57 (MQ=255) cGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTa > 1:2295196/1‑57 (MQ=255) cGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGAt > 1:3917915/1‑70 (MQ=255) gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCAtt < 1:3183343/41‑1 (MQ=255) gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCt < 1:1223115/51‑1 (MQ=255) gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCt < 1:3811193/51‑1 (MQ=255) aacaacATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaag > 1:1210611/1‑71 (MQ=255) aacaacACGCTGCGGTTTCGCTCGATCGTTTCGCATCATTTAGTCAAACCTGGTATGACACGGTAGATaag > 1:2076742/1‑71 (MQ=255) caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGt > 1:2362301/1‑62 (MQ=255) caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGt > 1:869886/1‑62 (MQ=255) caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagc > 1:2859246/1‑70 (MQ=255) caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagc > 1:379271/1‑70 (MQ=255) caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagc > 1:373791/1‑70 (MQ=255) aacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACg > 1:3929816/1‑59 (MQ=255) aacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAg < 1:939811/63‑1 (MQ=255) aacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagc > 1:2340909/1‑69 (MQ=255) acaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTa > 1:765120/1‑38 (MQ=255) acaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagcaag > 1:1409278/1‑71 (MQ=255) caTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTg > 1:4074747/1‑47 (MQ=255) caTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTg > 1:2269543/1‑47 (MQ=255) caTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGac > 1:781457/1‑54 (MQ=255) caTGCGGCGGTTTCGCGCGATCGTTTCGCGTCATTTAGTCAACCTTGGTAAGACACGGTAGATaagcaagc < 1:499808/71‑1 (MQ=255) caTGCGGCGGTTTCGATCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagcaagc > 1:2149760/1‑71 (MQ=255) | GTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGC > minE/69672‑69808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |