| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,554,520 | G→T | 22.3% | L40I (CTC→ATC) | envC ← | protease with a role in cell division |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,554,520 | 0 | G | T | 22.3% | 277.4 / 41.4 | 107 | L40I (CTC→ATC) | envC | protease with a role in cell division |
| Reads supporting (aligned to +/- strand): ref base G (46/37); new base T (10/14); total (56/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
CTTGCGCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA > minE/2554450‑2554589 | cTTGCGCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAg < 1:4211305/71‑1 (MQ=255) ttGCGCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGt > 1:4201397/1‑71 (MQ=255) gcgcGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTg > 1:4130984/1‑71 (MQ=255) cgcgTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTgg > 1:896916/1‑71 (MQ=255) gttgttgttGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCa > 1:3794050/1‑71 (MQ=255) ttgttgttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAg < 1:4678045/63‑1 (MQ=255) ttgttgttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTg < 1:2066824/66‑1 (MQ=255) ttgttgttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCa < 1:4668672/70‑1 (MQ=255) ttgttgCTTTTGGCGTACCGCGCGCTCTTTTGCTGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGc < 1:1794837/70‑1 (MQ=255) ttgttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGc < 1:4595933/70‑1 (MQ=255) gttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAgatttgattt > 1:1855138/1‑60 (MQ=255) gttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTc > 1:4425260/1‑70 (MQ=255) gttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTc < 1:1103938/70‑1 (MQ=255) ttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTTAATAGATTTGAGTTGGTCACGCTCAt < 1:2419195/71‑1 (MQ=255) ttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCa < 1:3767724/70‑1 (MQ=255) ttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCa < 1:326063/70‑1 (MQ=255) ttgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCa < 1:4065679/70‑1 (MQ=255) tgCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATc > 1:3451297/1‑71 (MQ=255) tttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCATCcgcg > 1:4367635/1‑71 (MQ=255) ttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTTAATAGATTTGAGTTGGTCACGCTCATCCGCGt > 1:1887018/1‑71 (MQ=255) ttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGt > 1:2265990/1‑56 (MQ=255) ttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCATCcgc < 1:4165973/69‑1 (MQ=255) ttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCcgcg < 1:3995570/70‑1 (MQ=255) ttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCcgc < 1:4254237/69‑1 (MQ=255) ttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGt > 1:4331507/1‑71 (MQ=255) ttGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGt > 1:1213677/1‑71 (MQ=255) ggCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTgg > 1:2230405/1‑71 (MQ=255) gCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTggg > 1:1301500/1‑71 (MQ=255) tACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGtt < 1:2490098/47‑1 (MQ=255) tACCGCGCGCTCTTTCGCGGCGATGTCAGCCTGAATGGATTTGAGTTGGTCACGCTCATCCGCGTGGGCg > 1:743041/1‑70 (MQ=255) ccGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaa < 1:1388438/71‑1 (MQ=255) ccGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaa < 1:3392000/71‑1 (MQ=255) ccGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaa < 1:2866088/71‑1 (MQ=255) ccGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGa > 1:1603027/1‑70 (MQ=255) ccGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGa < 1:1079718/70‑1 (MQ=255) ccGCGCGCTCTTTTGAGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGa > 1:1461269/1‑70 (MQ=255) gcgcgcTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCAc > 1:3809318/1‑49 (MQ=255) gcgcgcTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaaaa < 1:3892718/71‑1 (MQ=255) gcgcgcTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaaaa < 1:4418700/71‑1 (MQ=255) cgcgcTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAg < 1:40655/71‑1 (MQ=255) cgcgcTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTggg > 1:221495/1‑63 (MQ=255) cgcgcTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAg > 1:962510/1‑71 (MQ=255) gcTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCa < 1:3564815/50‑1 (MQ=255) ctctTTTGCGGCGATGTCAGCCTGAATAGATTTGAg > 1:147114/1‑36 (MQ=255) ctTTTGCGGCGATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGca > 1:954815/1‑70 (MQ=255) ttttGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGcac > 1:1012288/1‑70 (MQ=255) ttGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGgcg > 1:2418939/1‑65 (MQ=255) ttGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGgc > 1:1999336/1‑64 (MQ=255) ttGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGgc > 1:248888/1‑64 (MQ=255) ttGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCacaa > 1:2038301/1‑70 (MQ=255) tGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACg > 1:246218/1‑39 (MQ=255) tGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACg > 1:2287367/1‑39 (MQ=255) tGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCacaac < 1:4024044/70‑1 (MQ=255) cggcgATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCAcaacaa < 1:1851367/70‑1 (MQ=255) ggcgATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGaaaa < 1:2678541/57‑1 (MQ=255) gcgATGTCAGCCTGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATAc > 1:1027357/1‑71 (MQ=255) gATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGGGCACAACAATACGc > 1:2438586/1‑71 (MQ=255) gTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTg > 1:2474515/1‑42 (MQ=255) tCAGCCTGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCacaac > 1:1097254/1‑60 (MQ=255) tCAGCCTGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCAcaacaa < 1:2341326/62‑1 (MQ=255) tCAGCCTGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAg < 1:1604373/70‑1 (MQ=255) tCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCcgc < 1:1457150/38‑1 (MQ=255) tCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCcgc < 1:4187756/38‑1 (MQ=255) tCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCcg < 1:555603/37‑1 (MQ=255) tCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAgc > 1:4499832/1‑71 (MQ=255) cAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTTGGCGGAAAAGGCGCACAACAATa > 1:2547965/1‑63 (MQ=255) aGCCTGAATAGATTTGAGTTGGTCACGCTCATCcgcg > 1:392401/1‑37 (MQ=255) aGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCg > 1:2052607/1‑43 (MQ=255) aGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCAcaacaa < 1:1639565/60‑1 (MQ=255) ccTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCt < 1:3971908/70‑1 (MQ=255) cTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaaaa > 1:3378014/1‑45 (MQ=255) cTGAATAGATTTGAGTAGGTCACGCTCATCCGCGTGGGCGGaaaa > 1:4691571/1‑45 (MQ=255) tGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCacaac < 1:2744773/54‑1 (MQ=255) tGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCacaac < 1:3270942/54‑1 (MQ=255) tGAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCacaac < 1:3386612/54‑1 (MQ=255) gAATAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAg > 1:4407099/1‑44 (MQ=255) gAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaa < 1:3591107/41‑1 (MQ=255) aaTAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCacaac > 1:4617936/1‑52 (MQ=255) aaTAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAg < 1:500101/69‑1 (MQ=255) aaTAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGaa > 1:755876/1‑71 (MQ=255) aTAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAAc > 1:3934990/1‑71 (MQ=255) tAGATTTGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACg > 1:3569739/1‑71 (MQ=255) tAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaaa < 1:1998371/39‑1 (MQ=255) tAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAg < 1:1770063/67‑1 (MQ=255) aGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATa > 1:720627/1‑53 (MQ=255) gATTTGAGTTGGTGACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCt > 1:3660083/1‑71 (MQ=255) gATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGc < 1:1663812/70‑1 (MQ=255) gATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGc < 1:1192440/70‑1 (MQ=255) gATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGc < 1:940663/70‑1 (MQ=255) gATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCt > 1:2838258/1‑71 (MQ=255) gATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCt > 1:2449991/1‑71 (MQ=255) gATTTGAGTTGGTCACGCTAATCCGCGTGGGCGGAAAAGGCGCACAACAATa > 1:3616884/1‑52 (MQ=255) aTTTGAGTTGGTCACGCTCATCCGCGTGGGCGGaa < 1:2518606/35‑1 (MQ=255) tttGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTgg < 1:2572927/71‑1 (MQ=255) ttGATTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGc > 1:2101132/1‑71 (MQ=255) ttGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGc > 1:1545950/1‑71 (MQ=255) ttGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGc > 1:4170224/1‑71 (MQ=255) ttGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGc > 1:3990223/1‑71 (MQ=255) tGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCg > 1:1590347/1‑71 (MQ=255) aGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTa < 1:1150820/71‑1 (MQ=255) | CTTGCGCGTTGTTGTTGCTTTTGGCGTACCGCGCGCTCTTTTGCGGCGATGTCAGCCTGAATAGATTTGAGTTGGTCACGCTCATCCGCGTGGGCGGAAAAGGCGCACAACAATACGCCAGCGCTAAGAACGCTGGCGTA > minE/2554450‑2554589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |