BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,603,348	C→T	6.9%	P441P (CCG→CCA)	yhiP ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,603,348	0	C	T	6.9%	311.2 / 8.8	101	P441P (CCG→CCA)	yhiP	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (36/58); new base T (5/2); total (41/60)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 101 total aligned reads displayed.

GACCAGACCAATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGAACCACAT  >  minE/2603279‑2603416

gaccagaccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCg                                                                     >  1:3965520/1‑71 (MQ=255)

gaccagaccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCg                                                                     >  1:2330875/1‑71 (MQ=255)

 accagaccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCgg                                                                    <  1:349810/71‑1 (MQ=255)

  ccagaccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGc                                                                   <  1:3702244/71‑1 (MQ=255)

  caagaccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGc                                                                   <  1:1186986/69‑1 (MQ=255)

   cagaccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCa                                                                  >  1:4038084/1‑71 (MQ=255)

      accaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCgg                                                                    <  1:968381/66‑1 (MQ=255)

      accaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACcg                                                               <  1:1263780/71‑1 (MQ=255)

       ccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCgg                                                                    >  1:3988251/1‑65 (MQ=255)

       ccaATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACcgc                                                              >  1:4469188/1‑71 (MQ=255)

        caATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACcgcg                                                             >  1:4551243/1‑71 (MQ=255)

            cTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          >  1:818531/1‑70 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTaaa                                                        <  1:1043519/69‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTaaa                                                        <  1:2083810/69‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTaaa                                                        <  1:1490573/69‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTaa                                                         <  1:1648742/68‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTaa                                                         <  1:1843319/68‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          <  1:1861925/67‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          <  1:4652112/67‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          <  1:1999554/67‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAAt                                                       <  1:184411/70‑1 (MQ=255)

               aCCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAAt                                                       <  1:1982399/70‑1 (MQ=255)

                ccAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGt                                                     >  1:4010549/1‑71 (MQ=255)

                 cAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGc                                                                   <  1:2068025/56‑1 (MQ=255)

                 cAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGc                                                                   <  1:15578/56‑1 (MQ=255)

                 cAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          <  1:3939507/65‑1 (MQ=255)

                   aaCACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGc                                                  >  1:2288308/1‑71 (MQ=255)

                   aaCACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGc                                                  <  1:3940948/71‑1 (MQ=255)

                   aaCACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGc                                                  >  1:3721277/1‑71 (MQ=255)

                     cacGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCca                                                <  1:2230495/71‑1 (MQ=255)

                      acGTTGGTATAGACGGGCAACGTCTCAAGTGGATCGGTAATGTTGTCTGGTACTGCGGTAAATGTTGCcac                                               <  1:93216/71‑1 (MQ=255)

                      acGTTGGTATAGACGGGCAACGTCTCAAGTGGAGCGGTAATGTTGTCTGGTACTGCGGTAAATGTTGCcac                                               <  1:3073503/71‑1 (MQ=255)

                        gTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACAt                                             <  1:822036/71‑1 (MQ=255)

                        gTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACAt                                             <  1:4420413/71‑1 (MQ=255)

                        gTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACAt                                             <  1:2887222/71‑1 (MQ=255)

                            gTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          <  1:2229406/54‑1 (MQ=255)

                            gTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCca                                                <  1:3162340/64‑1 (MQ=255)

                            gTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAgc                                          <  1:2413015/70‑1 (MQ=255)

                             tataGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACTGCGGTAAATGTTGCcaca                                              >  1:4573113/1‑65 (MQ=255)

                             tataGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCcaca                                              >  1:303159/1‑65 (MQ=255)

                               taGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTaa                                                         <  1:1111688/52‑1 (MQ=255)

                                 gACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAgccgcc                                      >  1:3978275/1‑69 (MQ=255)

                                  aCGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAAt                                                       <  1:3141178/51‑1 (MQ=255)

                                  aCGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAg                                   >  1:2044725/1‑71 (MQ=255)

                                     ggCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGGCACATAGCCGCCCAGCaa                                <  1:855733/71‑1 (MQ=255)

                                      gCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAgccgcc                                      <  1:3007805/64‑1 (MQ=255)

                                      gCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAg                               <  1:1694433/71‑1 (MQ=255)

                                      gCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAg                               <  1:3267562/71‑1 (MQ=255)

                                       cAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAg                               >  1:4345756/1‑70 (MQ=255)

                                       cAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGa                              <  1:504914/71‑1 (MQ=255)

                                        aaCGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATa                                            >  1:607096/1‑56 (MQ=255)

                                         aCGTCTCAAGTGGATCGGTAATGTTGTCTGGTACTGCGGTAAATGTTGCCACATAGCCGCCCAGCaa                                >  1:1377851/1‑67 (MQ=255)

                                         aCGTCTCAAGTGGATCGGTAATGTTGTCTGGTACTGCGGTAAATGTTGCCACATAGCCGCCCAGCaa                                >  1:1601761/1‑67 (MQ=255)

                                         aCGTCTCAAGTGGATCGGTAATGTTGTCTGGTACTGCGGTAAATGTTGCCACATAGCCGCCCAGCaa                                >  1:3062828/1‑67 (MQ=255)

                                         aCGTCTCAAGTGGATCGGTAATGTTGTCTGGTACTGCGGTAAATGTTGCCACATAGCCGCCCAGCaa                                >  1:2974053/1‑67 (MQ=255)

                                         aCGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          <  1:4439310/41‑1 (MQ=255)

                                         aCGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAAc                            >  1:3017917/1‑71 (MQ=255)

                                          cGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAAcg                           <  1:759902/71‑1 (MQ=255)

                                          cGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAAcg                           <  1:3884345/71‑1 (MQ=255)

                                           gTCTCAAGCGGATCGGTAATGTTGTCCGGCACCTCGGTAAATGTTGCCACATAGCCGCCCa                                    >  1:365496/1‑61 (MQ=255)

                                           gTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAAcgc                          >  1:2094868/1‑71 (MQ=255)

                                            tctcAAGTGGATCGGTAATGTTGTCTGGTACTGCGGTAAATGTTGCCACAt                                             >  1:857072/1‑51 (MQ=255)

                                            tctcAAGCGGATCGGTAATGTTGTCCGGCACCGCTGTa                                                          <  1:3964316/38‑1 (MQ=255)

                                            tctcAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTa                                                          <  1:2470818/38‑1 (MQ=255)

                                            tctcAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAAcgcg                         <  1:2851158/71‑1 (MQ=255)

                                            tctcAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAAcgcg                         >  1:1500796/1‑71 (MQ=255)

                                              tcAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAAt                                                       >  1:4173192/1‑39 (MQ=255)

                                              tcAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCgg                        >  1:1414880/1‑70 (MQ=255)

                                              tcAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCgg                        >  1:1823065/1‑70 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCca                                                >  1:272680/1‑44 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAg                               <  1:2573887/61‑1 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAAcgcg                         >  1:2387303/1‑67 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCa                      <  1:853357/70‑1 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCa                      <  1:305800/70‑1 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAg                     <  1:524230/71‑1 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAg                     >  1:3295283/1‑71 (MQ=255)

                                                aaGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAg                     >  1:414878/1‑71 (MQ=255)

                                                 aGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGc                    <  1:582622/71‑1 (MQ=255)

                                                   cGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCa                      >  1:1918217/1‑67 (MQ=255)

                                                   cGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCt                  <  1:3155459/71‑1 (MQ=255)

                                                   cGGATCGGTAATGTTGTCCGGCACCGCCGTAAATGTTg                                                   >  1:114533/1‑38 (MQ=255)

                                                      aTCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCg               <  1:4319031/71‑1 (MQ=255)

                                                       tCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGt              <  1:3396162/71‑1 (MQ=255)

                                                       tCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGt              <  1:186035/71‑1 (MQ=255)

                                                        cGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGt              >  1:3364389/1‑70 (MQ=255)

                                                        cGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGt              <  1:1039848/70‑1 (MQ=255)

                                                          gaaaTGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAg           <  1:3542976/69‑1 (MQ=255)

                                                           tAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAg                                   <  1:1682238/46‑1 (MQ=255)

                                                             aTGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGaa                             <  1:2300941/50‑1 (MQ=255)

                                                             aTGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGaa        <  1:1227463/71‑1 (MQ=255)

                                                             aTGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGaa        <  1:1241793/71‑1 (MQ=255)

                                                             aTGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGa         <  1:2515223/70‑1 (MQ=255)

                                                             aTGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGa         <  1:1720201/70‑1 (MQ=255)

                                                               gttgtCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCa            >  1:3852678/1‑65 (MQ=255)

                                                                ttgtCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCa                                    >  1:437763/1‑40 (MQ=255)

                                                                ttgtCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGAAcc      <  1:4554849/70‑1 (MQ=255)

                                                                ttgtCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGAACca     >  1:133142/1‑71 (MQ=255)

                                                                ttgtCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGAACca     >  1:1957853/1‑71 (MQ=255)

                                                                  gtCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGAACcac    >  1:1441264/1‑70 (MQ=255)

                                                                   tCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGAACCACAt  <  1:4562015/71‑1 (MQ=255)

GACCAGACCAATCTTACCAAACACGTTGGTATAGACGGGCAACGTCTCAAGCGGATCGGTAATGTTGTCCGGCACCGCGGTAAATGTTGCCACATAGCCGCCCAGCAAGAACGCGGCAGCCTGCGTCAGGAACCACAT  >  minE/2603279‑2603416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`