| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,699,171 | C→T | 6.3% | T16T (ACC→ACT) | yrfC → | predicted fimbrial assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,699,171 | 0 | C | T | 6.3% | 195.0 / 2.6 | 63 | T16T (ACC→ACT) | yrfC | predicted fimbrial assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (29/30); new base T (2/2); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
CGCTTGGGAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCTAATA > minE/2699105‑2699240 | cgcTTGGGAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCtt > 1:2176815/1‑71 (MQ=255) gcTTGGGAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCttt > 1:3205063/1‑71 (MQ=255) gcTTGGGAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCtt < 1:294072/70‑1 (MQ=255) tGGGAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCt > 1:4365533/1‑71 (MQ=255) gggAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTg > 1:4636450/1‑71 (MQ=255) ggAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCt < 1:775736/69‑1 (MQ=255) gAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCg > 1:1761992/1‑71 (MQ=255) aGGCGGACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCttt < 1:2925496/71‑1 (MQ=255) ggCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGttt > 1:2359874/1‑71 (MQ=255) gCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCt < 1:514854/64‑1 (MQ=255) tACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCTACAGCAACGCCGGACCGCtt < 1:3798977/57‑1 (MQ=255) tACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTgg < 1:2306804/71‑1 (MQ=255) cTGATGAACCCGCGAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGtt > 1:1631137/1‑71 (MQ=255) cTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTc < 1:2453437/58‑1 (MQ=255) cTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGtt > 1:2496444/1‑71 (MQ=255) cTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGtt > 1:2451394/1‑71 (MQ=255) gatgaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCTGGACTGCTTTTCTTCGttt > 1:2816422/1‑63 (MQ=255) gatgaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACTGCTTTTCTTCGttt > 1:2139866/1‑63 (MQ=255) atgaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCg > 1:2407948/1‑49 (MQ=255) atgaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTg < 1:4592762/69‑1 (MQ=255) atgaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCt < 1:2136395/71‑1 (MQ=255) atgaACACGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCt > 1:280425/1‑71 (MQ=255) tgaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTg < 1:1602654/71‑1 (MQ=255) tgaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTg < 1:1325563/71‑1 (MQ=255) gaACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCt < 1:3550827/69‑1 (MQ=255) aaCCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCtttt > 1:1968420/1‑51 (MQ=255) aaCCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGa < 1:3300654/70‑1 (MQ=255) aCCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATg < 1:1531804/71‑1 (MQ=255) cGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTc < 1:1612067/71‑1 (MQ=255) gCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCg < 1:926482/71‑1 (MQ=255) cAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGa > 1:3149100/1‑63 (MQ=255) attaattTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGt < 1:2896231/55‑1 (MQ=255) attaattTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTgc < 1:3161564/71‑1 (MQ=255) attaattTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTgc < 1:2547736/71‑1 (MQ=255) taattTTTTGCCCTGGCAACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTgcgc > 1:4337274/1‑71 (MQ=255) aattTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTg > 1:3132480/1‑67 (MQ=255) ttttttGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTg < 1:4389614/48‑1 (MQ=255) tGCCCTGGCGACAGCAACGCCGGACTGCTTTTCTTCGTTTCTGGTTGCTGATGTTCGTTGCGCCTctgct < 1:3663990/70‑1 (MQ=255) ggCGACAGCAACGCCGGACTGCTTTTCTTCGTTTCt < 1:2154746/36‑1 (MQ=38) gCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCg > 1:4155724/1‑71 (MQ=255) cGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGt > 1:239/1‑71 (MQ=255) aGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCgg > 1:312980/1‑70 (MQ=255) aGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCgg > 1:1219729/1‑70 (MQ=255) cAACGCCGGACCGCTTTTCTGGGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGAt < 1:478031/71‑1 (MQ=255) cAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGa > 1:3810207/1‑70 (MQ=255) cAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGa > 1:3815601/1‑70 (MQ=255) cAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGAt < 1:2763628/71‑1 (MQ=255) cAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGAt < 1:745501/71‑1 (MQ=255) aaCGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGAt > 1:3181253/1‑70 (MQ=255) aaCGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGAt > 1:2837487/1‑70 (MQ=255) aCGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGt < 1:931360/63‑1 (MQ=255) cGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTctgctgc < 1:3896442/55‑1 (MQ=255) cGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAAc < 1:395812/71‑1 (MQ=255) cGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAAc > 1:2098191/1‑71 (MQ=255) gCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACg > 1:2600560/1‑71 (MQ=255) cGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATaa > 1:2282436/1‑67 (MQ=255) cGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCt < 1:4034423/71‑1 (MQ=255) cGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCt < 1:4245627/71‑1 (MQ=255) cGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCt < 1:767364/71‑1 (MQ=255) ggACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGATGGCCGTCGGGATAACGCt > 1:4294051/1‑70 (MQ=255) gACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTctgctgc > 1:3962830/1‑50 (MQ=255) gACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTctgctgc > 1:2313065/1‑50 (MQ=255) gACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCTaa < 1:1887521/71‑1 (MQ=255) ccGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGAt > 1:2523840/1‑61 (MQ=255) ccGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCt < 1:1228106/67‑1 (MQ=255) ccGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCTAATa > 1:2502228/1‑71 (MQ=255) | CGCTTGGGAAGGCGTACTGATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGTTGCGCCTCTGCTGCTGGCCGTCGGGATAACGCTAATA > minE/2699105‑2699240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |