| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,858,365 | G→A | 7.1% | T213T (ACG→ACA) | amiB → | N‑acetylmuramoyl‑l‑alanine amidase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,858,365 | 0 | G | A | 7.1% | 214.8 / 4.9 | 70 | T213T (ACG→ACA) | amiB | N‑acetylmuramoyl‑l‑alanine amidase II |
| Reads supporting (aligned to +/- strand): ref base G (30/35); new base A (3/2); total (33/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
ATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTA > minE/2858295‑2858435 | aTAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcg < 1:4127050/71‑1 (MQ=255) tAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcgc > 1:243696/1‑71 (MQ=255) aaaaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcgcg > 1:1019880/1‑71 (MQ=255) aaaaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcgcg > 1:3055129/1‑71 (MQ=255) aaaaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcgcg > 1:1732525/1‑71 (MQ=255) aaaaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcgcg > 1:4312116/1‑71 (MQ=255) aaaTTATCATCGCTATTGATG‑CGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCgg < 1:3623281/70‑1 (MQ=255) aaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCggg < 1:36626/71‑1 (MQ=255) aaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCgg < 1:484878/70‑1 (MQ=255) aTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCgg > 1:1612290/1‑69 (MQ=255) tcatcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGaaa < 1:3512436/71‑1 (MQ=255) tcatcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGaaa < 1:3900724/71‑1 (MQ=255) tcatcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGaa < 1:3709452/70‑1 (MQ=255) tcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAAt < 1:1738574/71‑1 (MQ=255) ttGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGaaa < 1:922074/62‑1 (MQ=255) ttGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAc > 1:1919142/1‑70 (MQ=255) ttGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAc > 1:3269390/1‑70 (MQ=255) ttGATGCCGGACACGGCGGTCAGGACCCTGGCGATATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAc < 1:1047205/70‑1 (MQ=255) tGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAcca > 1:109300/1‑71 (MQ=255) tGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAc > 1:2556947/1‑69 (MQ=255) gATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcgc > 1:3471248/1‑52 (MQ=255) gATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTc > 1:1329670/1‑66 (MQ=255) gATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccat > 1:4345185/1‑71 (MQ=255) aTGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatc > 1:2889014/1‑71 (MQ=255) ccGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatcgcc < 1:3144231/71‑1 (MQ=255) ggACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatcgccat > 1:3103203/1‑71 (MQ=255) cggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTa < 1:1395357/71‑1 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatcgcc < 1:3619113/59‑1 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTaaa < 1:3825678/71‑1 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTa < 1:4096391/69‑1 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTa < 1:2634266/69‑1 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTa < 1:1706670/69‑1 (MQ=255) tCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTaaa < 1:4157100/70‑1 (MQ=255) cAGGACCCTGGCGCTATCGGCCCTGGTGGTACACGGGAGAAAAATGTCAccatcgccatt > 1:853284/1‑59 (MQ=255) cAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAtt > 1:2536364/1‑71 (MQ=255) aGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAtt < 1:1172053/70‑1 (MQ=255) aGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAtt < 1:1950281/70‑1 (MQ=255) aGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTg > 1:1560801/1‑71 (MQ=255) ggACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGc < 1:3356518/71‑1 (MQ=255) gACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCg > 1:515132/1‑71 (MQ=255) aCCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCg > 1:2300869/1‑70 (MQ=255) ccTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTAc < 1:2040667/71‑1 (MQ=255) ggCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACttt > 1:2894024/1‑71 (MQ=255) ggCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACttt > 1:1435308/1‑71 (MQ=255) gcgcTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACttt < 1:3903485/70‑1 (MQ=255) cgcTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGc > 1:934213/1‑71 (MQ=255) cTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTc < 1:4288368/71‑1 (MQ=255) cTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTc < 1:2292727/71‑1 (MQ=255) tATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTc > 1:4072431/1‑70 (MQ=255) aTCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTa > 1:830566/1‑61 (MQ=255) aTCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTa > 1:3930457/1‑61 (MQ=255) aTCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCaa > 1:3197568/1‑71 (MQ=255) aTCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCaa > 1:252820/1‑71 (MQ=255) tCGGCCCTGGTGGTACACGGGAGAAAAATGTCACCATCGCCATTGCGCGTAAAttac < 1:1286003/57‑3 (MQ=255) tCGGCCCTGGTGGTACACGGGAGAAAAATGTCACCATCGCCATTGCGCGTAAAttac < 1:2154138/57‑3 (MQ=255) tCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCaa < 1:1314699/70‑1 (MQ=255) tCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCa < 1:2891506/69‑1 (MQ=255) cGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAAt > 1:400803/1‑70 (MQ=255) cGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAAt > 1:2408133/1‑70 (MQ=255) ggCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACtt > 1:4341446/1‑61 (MQ=255) ggCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACtt > 1:2423720/1‑61 (MQ=255) ggCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATg < 1:414418/70‑1 (MQ=255) ctggtggtACACGGGAGAAAAATGTCACCATCGCCATTGCGCGTAAATTACGTACCTTGCTCAATAACGa > 1:1779576/3‑70 (MQ=255) ctggtggtACACGGGAGAAAAATGTCACCATCGCCATTGCGCGTAAATTACGTACCTTGCTCAATAACGAt > 1:4387568/3‑71 (MQ=255) ccGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGAt < 1:2343633/71‑1 (MQ=255) cGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGAt < 1:1258117/70‑1 (MQ=255) cGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGAt < 1:3320091/70‑1 (MQ=255) ggtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTaaa > 1:2900503/1‑45 (MQ=255) ggtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTATATTGAGTACTTTGCTCAATGACGATcc > 1:1251470/1‑71 (MQ=255) ggtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATcc < 1:603375/71‑1 (MQ=255) ggtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATcc < 1:2190640/71‑1 (MQ=255) gtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATcc > 1:2583043/1‑70 (MQ=255) gtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATcc > 1:2290739/1‑70 (MQ=255) tggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGa < 1:2689720/71‑1 (MQ=255) tggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGa < 1:3836976/71‑1 (MQ=255) ggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAt > 1:3787403/1‑71 (MQ=255) tACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGa < 1:490466/68‑1 (MQ=255) cgcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAt > 1:3351669/1‑39 (MQ=255) cgcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTc < 1:2071487/53‑1 (MQ=255) cgcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAt > 1:3385930/1‑67 (MQ=255) cgcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGtt > 1:2096148/1‑70 (MQ=255) cgcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGtt > 1:2528724/1‑70 (MQ=255) gcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTa < 1:1518090/71‑1 (MQ=255) gcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTa < 1:2125246/71‑1 (MQ=255) | ATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTA > minE/2858295‑2858435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |