BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,910,046	A→C	5.2%	V346V (GTA→GTC)	mgtA →	magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,910,046	0	A	C	5.2%	248.5 / 8.1	97	V346V (GTA→GTC)	mgtA	magnesium transporter
Reads supporting (aligned to +/- strand): ref base A (39/53); new base C (2/3); total (41/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 101 total aligned reads displayed.

GGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATAT  >  minE/2909977‑2910114

ggTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTaa                                                                     <  1:3684156/71‑1 (MQ=255)

ggTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTa                                                                      <  1:4661585/70‑1 (MQ=255)

ggTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTa                                                                      <  1:3500744/70‑1 (MQ=255)

  tCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAg                                                                   <  1:235364/71‑1 (MQ=255)

  tCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAg                                                                   <  1:240826/71‑1 (MQ=255)

         aGGCCTAATGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCg                                                             >  1:3599319/1‑70 (MQ=255)

         aGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGAGCGCGGCGCAGTAAAGCTGTCg                                                             >  1:4120909/1‑70 (MQ=255)

          ggCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGa                                                            <  1:2850571/70‑1 (MQ=255)

          ggCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGa                                                            <  1:3307883/70‑1 (MQ=255)

          ggCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGa                                                            >  1:4252569/1‑70 (MQ=255)

          ggCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGATGTCGa                                                            <  1:4219598/70‑1 (MQ=255)

           gCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAg                                                                   <  1:644920/62‑1 (MQ=255)

           gCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAg                                                                   <  1:2057299/62‑1 (MQ=255)

           gCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAg                                                                   <  1:4358515/62‑1 (MQ=255)

           gCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGaaa                                                          <  1:4123414/71‑1 (MQ=255)

            ccTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGaaa                                                          >  1:4643111/1‑70 (MQ=255)

             ttAACGCCAGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGTGGCGCAGTCAAGTTGTCGaaa                                                          >  1:2386504/2‑69 (MQ=255)

             ttAACGCCAGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGTGGCGCAGTCAAGTTGTCGaaa                                                          >  1:2381115/2‑69 (MQ=255)

             cTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACa                                                        <  1:615493/71‑1 (MQ=255)

              tAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGaa                                                           <  1:1104865/67‑1 (MQ=255)

                aCGCCGGAAATGTTGCCGATGATTGTTACCTCTACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      <  1:3157112/70‑1 (MQ=255)

                aCGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGaaa                                                          >  1:2026126/1‑66 (MQ=255)

                aCGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      >  1:4326121/1‑70 (MQ=255)

                aCGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      <  1:1039953/70‑1 (MQ=255)

                aCGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      >  1:1544116/1‑70 (MQ=255)

                aCGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      <  1:1009311/70‑1 (MQ=255)

                   ccGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGt                                                  <  1:3578275/71‑1 (MQ=255)

                   ccGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGt                                                  >  1:2545152/1‑71 (MQ=255)

                    cGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTg                                                 <  1:2107132/71‑1 (MQ=255)

                    cGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTg                                                 <  1:3738283/71‑1 (MQ=255)

                    cGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTg                                                 <  1:3903336/71‑1 (MQ=255)

                    cGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTg                                                 <  1:4428809/71‑1 (MQ=255)

                    cGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTg                                                 <  1:1216295/71‑1 (MQ=255)

                     ggAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGa                                                >  1:3373878/1‑71 (MQ=255)

                      gAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGAt                                               >  1:2362990/1‑71 (MQ=255)

                          tGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGt                                            >  1:2929383/1‑70 (MQ=255)

                           gTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTc                                           >  1:239013/1‑70 (MQ=255)

                            ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGTCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCa                                          <  1:671322/70‑1 (MQ=255)

                            ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTa                                                                      <  1:2889043/42‑1 (MQ=255)

                            ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCaa                                         <  1:3651492/71‑1 (MQ=255)

                            ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCaa                                         <  1:2858664/71‑1 (MQ=255)

                            ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCaa                                         <  1:2685087/71‑1 (MQ=255)

                            ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCa                                          <  1:562136/70‑1 (MQ=255)

                            ttGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCa                                          <  1:463076/70‑1 (MQ=255)

                              gCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCaaa                                        >  1:3451520/1‑70 (MQ=255)

                               ccGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACa                                      <  1:1880165/71‑1 (MQ=255)

                               ccGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACa                                      >  1:2720015/1‑71 (MQ=255)

                                cGATGATTGTTACCTCGACTCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      <  1:2721881/54‑1 (MQ=255)

                                cGATGATTGTTACCTCGACGCTGGCGCGTGGCGCAGTCAAGTTGTCGAAAc                                                         <  1:1901069/51‑1 (MQ=255)

                                cGATGATTGTTACCTCGACGCTGGCGCGTGGCGCAGTCAAGTTGTCGAAAc                                                         <  1:3306375/51‑1 (MQ=255)

                                 gatgatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCaaa                                        <  1:2127847/67‑1 (MQ=255)

                                  atgatTGTTACCTCGACGCTGGCGCGTGGCGCAGTCAAGTTGTCGAAAc                                                         <  1:4590905/49‑1 (MQ=255)

                                  atgatTGTTACCTCGACGCTGGCGCGCGGCGCATTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCt                                   >  1:861799/1‑71 (MQ=255)

                                  atgatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      <  1:705817/52‑1 (MQ=255)

                                  atgatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCt                                   >  1:3359245/1‑71 (MQ=255)

                                  atgatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCt                                   >  1:143791/1‑71 (MQ=255)

                                   tgatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTg                                  <  1:531816/71‑1 (MQ=255)

                                   agatTGTTACCTCGACGCTGGCGCGCGGCCCAGTAAAGc                                                                  >  1:2642516/2‑39 (MQ=39)

                                    gatTGTTACCTCGACGCTGTCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTg                                  >  1:3108747/1‑70 (MQ=255)

                                    gatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGa                                                            <  1:3913454/44‑1 (MQ=255)

                                    gatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTg                                  >  1:1388070/1‑70 (MQ=255)

                                    gatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTg                                  >  1:160976/1‑70 (MQ=255)

                                    gatTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTg                                  >  1:4143644/1‑70 (MQ=255)

                                     atTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGa                                                      <  1:1044164/49‑1 (MQ=255)

                                     atTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTgg                                 <  1:1354273/70‑1 (MQ=255)

                                     atTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGa                                <  1:192457/71‑1 (MQ=255)

                                      ttGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGaaa                                                    >  1:4532606/1‑50 (MQ=255)

                                      ttGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGaaa                                                    >  1:4111310/1‑50 (MQ=255)

                                      ttGTTACCTCGACGCTGGCGCGCGGCCCAGTAAAGCTGTCGAAACAGAAAg                                                   <  1:2578572/51‑1 (MQ=255)

                                       tGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACAt                                     >  1:4597679/1‑64 (MQ=255)

                                        gTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGaa                                                     >  1:1997277/1‑47 (MQ=255)

                                        gTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGaa                                                     >  1:4561789/1‑47 (MQ=255)

                                        gTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGaa                                                     >  1:2636248/1‑47 (MQ=255)

                                        gTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATg                              <  1:3727291/70‑1 (MQ=255)

                                          tACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGaaa                                                          >  1:3360145/1‑40 (MQ=255)

                                           aCCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTa                           <  1:4013854/70‑1 (MQ=255)

                                           aCCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTAt                          >  1:3142645/1‑71 (MQ=255)

                                            ccTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTAtt                         <  1:1411317/71‑1 (MQ=255)

                                                gACGCTGGCGCGGGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAg                      >  1:2793213/1‑70 (MQ=255)

                                                gACGCTGGCGCGCGGGGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAg                      >  1:1337076/1‑70 (MQ=255)

                                                gACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAg                      >  1:3375247/1‑70 (MQ=255)

                                                gACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAg                      >  1:3731149/1‑70 (MQ=255)

                                                 aCGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGa                     <  1:4223449/70‑1 (MQ=255)

                                                    cTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACtt                 <  1:2011477/71‑1 (MQ=255)

                                                      ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTc                                           >  1:3305153/1‑43 (MQ=255)

                                                       tcgcgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTg               <  1:1232690/69‑1 (MQ=255)

                                                       gcgcgcGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACttt                <  1:1311047/69‑1 (MQ=255)

                                                       gcgcgcGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTg               <  1:1623193/70‑1 (MQ=255)

                                                       gcgcgcGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTg               <  1:3789960/70‑1 (MQ=255)

                                                          cgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc           <  1:4241744/71‑1 (MQ=255)

                                                          cgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc           <  1:4517149/71‑1 (MQ=255)

                                                          cgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGgcgc           <  1:1763110/71‑1 (MQ=255)

                                                           gcggcgCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTgg                                 >  1:1951002/1‑48 (MQ=255)

                                                               cgcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATgg      <  1:519611/71‑1 (MQ=255)

                                                                 cAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGa     >  1:455486/1‑70 (MQ=255)

                                                                 cAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGa     >  1:3114228/1‑70 (MQ=255)

                                                                 cAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGa     >  1:3057025/1‑70 (MQ=255)

                                                                  aGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTg                                  <  1:3932181/40‑1 (MQ=255)

                                                                  aGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGata   <  1:312752/71‑1 (MQ=255)

                                                                   gTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGatat  <  1:1675205/71‑1 (MQ=255)

GGTCGCGGTAGGCCTAACGCCGGAAATGTTGCCGATGATTGTTACCTCGACGCTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATAT  >  minE/2909977‑2910114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`