| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,963,081 | G→A | 20.4% | A92V (GCG→GTG) | yjjI ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,963,081 | 0 | G | A | 20.4% | 197.5 / 42.5 | 93 | A92V (GCG→GTG) | yjjI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (44/30); new base A (6/13); total (50/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
TCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGC > minE/2963013‑2963149 | tCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTcgcg > 1:1335659/1‑71 (MQ=255) cGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCgcgc > 1:2006339/1‑71 (MQ=255) cGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCgcgc > 1:745560/1‑71 (MQ=255) gATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCgcgc > 1:2575719/1‑70 (MQ=255) gATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCgcgc < 1:1477250/70‑1 (MQ=255) gATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGcc > 1:3853388/1‑71 (MQ=255) gATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGcc > 1:1462926/1‑71 (MQ=255) aTGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTcg < 1:3661624/66‑1 (MQ=255) aTGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCt < 1:236193/71‑1 (MQ=255) tgtgACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCtt > 1:1510348/1‑71 (MQ=255) gtgACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtc > 1:4165098/1‑71 (MQ=255) gtgACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtc > 1:3925189/1‑71 (MQ=255) tgACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCACGCCTtc < 1:3636570/70‑1 (MQ=255) ccGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCACGCCTtccagc > 1:1627725/1‑71 (MQ=255) ccGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCACGCCTtccagc > 1:2546770/1‑71 (MQ=255) accaCGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCACGCCTtccagc < 1:1813774/68‑1 (MQ=255) cGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccagct > 1:4612923/1‑71 (MQ=255) aCGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccagctc > 1:3836030/1‑70 (MQ=255) aCGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCa > 1:231562/1‑71 (MQ=255) cGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccagctc > 1:1937219/1‑64 (MQ=255) cGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCAt < 1:4243305/71‑1 (MQ=255) gtggtgGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCAtt < 1:1356695/71‑1 (MQ=255) tggtggTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAATATCTTTCGCGCCTtccagc > 1:3342410/1‑60 (MQ=255) tggtggTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccagctcc < 1:3132618/63‑1 (MQ=255) tggtggTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCAtt > 1:3959597/1‑70 (MQ=255) tggtggTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTc > 1:4613432/1‑71 (MQ=255) ggtggtAAAGAATGGTCAGCAGAGAGAGTGCGTCATCATGATCTTTCACGCCTTCCAGCTCCAGCCATTCg > 1:2971380/1‑71 (MQ=255) ggtAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGaa > 1:2543239/1‑71 (MQ=255) gtAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAAc > 1:1417160/1‑71 (MQ=255) gtAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAAc < 1:51635/71‑1 (MQ=255) tAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAAcc < 1:1995941/71‑1 (MQ=255) aaaGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCgcgc > 1:257395/1‑45 (MQ=255) aaaGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccagctcca > 1:2261439/1‑57 (MQ=255) aaGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTcgc > 1:3044591/1‑42 (MQ=255) aaGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccagctcca < 1:500461/56‑1 (MQ=255) aaGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGt < 1:4387479/71‑1 (MQ=255) aaGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGt > 1:851537/1‑71 (MQ=255) aGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGtt < 1:212843/71‑1 (MQ=255) gAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccagctccagc > 1:1216561/1‑56 (MQ=255) gAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGtt > 1:4086021/1‑70 (MQ=255) aaTGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtc < 1:712952/45‑1 (MQ=255) aTGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCGTGCCAtt < 1:4646502/58‑1 (MQ=255) tGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTcgcg > 1:3971759/1‑38 (MQ=255) tGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtccag < 1:3111019/46‑1 (MQ=255) agcagAGAGAGTGCGTCATCAAGATCTTTCGCGCCTtcc > 1:1976878/1‑39 (MQ=255) agcagAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaaa < 1:3028589/71‑1 (MQ=255) agcagAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaaa < 1:2120713/71‑1 (MQ=255) agcagAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa > 1:1741680/1‑70 (MQ=255) agcagAGAGAGTGCGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa < 1:3914742/70‑1 (MQ=255) agcagAGAGAGTGCGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa < 1:1021572/70‑1 (MQ=255) gcagAGAGAGTGCGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa < 1:3236657/69‑1 (MQ=255) cagAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc < 1:479153/71‑1 (MQ=255) cagAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc < 1:4189372/71‑1 (MQ=255) cagAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc < 1:2436419/71‑1 (MQ=255) agagagagTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc < 1:2490326/70‑1 (MQ=255) agagagagTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCAATTCGGAACCGTTCGCCAGAAAACg > 1:3699337/1‑71 (MQ=255) gagagagTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGtt > 1:3107487/1‑58 (MQ=255) agagTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCg > 1:3097057/1‑71 (MQ=255) agagTGCGACATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGaa > 1:2420877/1‑50 (MQ=255) gagTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGt < 1:4640047/71‑1 (MQ=255) agTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTa < 1:12408/71‑1 (MQ=255) agTGCGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTa < 1:2521398/71‑1 (MQ=255) agTGCGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTa < 1:793094/71‑1 (MQ=255) tGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGa < 1:1912678/45‑1 (MQ=255) cGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGt > 1:3066841/1‑66 (MQ=255) cGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCg > 1:4648978/1‑71 (MQ=255) cGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCg > 1:877572/1‑71 (MQ=255) cGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCg > 1:904190/1‑71 (MQ=255) cGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCg > 1:1546386/1‑71 (MQ=255) cGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATc < 1:1770099/70‑1 (MQ=255) cGTCATCAAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATc < 1:2955811/70‑1 (MQ=255) tcatcaAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCggg > 1:3970240/1‑71 (MQ=255) atcaAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCgg > 1:4259086/1‑38 (MQ=255) atcaAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt < 1:1048056/70‑1 (MQ=255) atcaAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt > 1:489101/1‑70 (MQ=255) atcaAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt < 1:109120/70‑1 (MQ=255) atcaAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt < 1:4145892/70‑1 (MQ=255) atcaAGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt > 1:524921/1‑70 (MQ=255) tcaAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa < 1:3864665/52‑1 (MQ=255) tcaAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt > 1:3837039/1‑69 (MQ=255) caAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt > 1:2403744/1‑68 (MQ=255) caAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt > 1:1155938/1‑68 (MQ=255) caAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGt > 1:3272021/1‑68 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAAc > 1:1083832/1‑38 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa > 1:3998015/1‑50 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa < 1:76228/50‑1 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa < 1:70668/50‑1 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGt < 1:2833965/59‑1 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGa > 1:538139/1‑71 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGa < 1:826754/71‑1 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGa < 1:3480245/71‑1 (MQ=255) aaGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGa < 1:2433099/71‑1 (MQ=255) aaGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGaa > 1:169190/1‑37 (MQ=255) aaGATCTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATAGGGTAAg < 1:2042016/70‑1 (MQ=255) aGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa < 1:3016332/49‑1 (MQ=255) tCTTTCGCGCCTTCCAGCTCCAGCCATTCGGATCCGTTCGCCAg > 1:4029259/1‑44 (MQ=255) cTTTCACGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAg > 1:1896283/1‑71 (MQ=255) tttCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAg > 1:1618211/1‑70 (MQ=255) ttCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACa > 1:2102725/1‑66 (MQ=255) tCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAgcgc > 1:541971/1‑71 (MQ=255) | TCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGC > minE/2963013‑2963149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |