| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 244,761 | C→T | 39.1% | G221R (GGG→AGG) | sbcD ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 244,761 | 0 | C | T | 39.1% | 50.9 / 71.5 | 69 | G221R (GGG→AGG) | sbcD | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (18/24); new base T (13/14); total (31/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01 | |||||||||||
ATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGT > minE/244697‑244829 | aTGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAgcgc > 1:3979400/1‑71 (MQ=255)aTGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAgcgc < 1:3684698/71‑1 (MQ=255) tGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAgcgc > 1:710494/1‑70 (MQ=255) tGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGcgcg < 1:3381322/71‑1 (MQ=255) gggAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:58207/71‑1 (MQ=255) gggAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:2196045/71‑1 (MQ=255) gAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTa < 1:2556003/71‑1 (MQ=255) gAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTa < 1:3583324/71‑1 (MQ=255) aGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAg > 1:320207/1‑71 (MQ=255) gCCGCAATAGCGAACATGTTCCATGTCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGt > 1:4237853/1‑71 (MQ=255) gCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCgg > 1:1912817/1‑71 (MQ=255) aaTAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCt > 1:4232721/1‑71 (MQ=255) aGCGAACATGTTCCATGCCGCCATTAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtggt > 1:4614247/1‑71 (MQ=255) aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtggt > 1:4608749/1‑71 (MQ=255) gCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg > 1:1621678/1‑61 (MQ=255) gCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg > 1:2118105/1‑61 (MQ=255) cATGTTCCATGCCGCCAATAATCTGTGCGCGGTTAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAg < 1:2305450/71‑1 (MQ=255) cATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAg < 1:4578208/71‑1 (MQ=255) cATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAg > 1:3057552/1‑71 (MQ=255) tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:1636096/71‑1 (MQ=255) tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGt < 1:3763664/70‑1 (MQ=255) tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg < 1:2507124/54‑1 (MQ=255) tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:955570/71‑1 (MQ=255) tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:2049821/71‑1 (MQ=255) gTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtggtg > 1:270619/1‑63 (MQ=255) gTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGttt > 1:276112/1‑71 (MQ=255) ttCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGttt < 1:4276158/70‑1 (MQ=255) ttCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:4287465/49‑1 (MQ=255) ttCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg < 1:2116602/52‑1 (MQ=255) cATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTtgt < 1:3063905/70‑1 (MQ=255) aTGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGc > 1:1377725/1‑71 (MQ=255) tGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:1139409/44‑1 (MQ=255) gccgccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:1133858/43‑1 (MQ=255) ccgccAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGcc > 1:2350170/1‑69 (MQ=255) ccgccAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCgg < 1:3996868/71‑1 (MQ=255) gccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGa > 1:1493106/1‑70 (MQ=255) cAATAATCTGTGCGCGGTGAATATGCCTGAGcgcg < 1:3229520/35‑1 (MQ=255) cAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAc < 1:3507617/71‑1 (MQ=255) aataatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTtgtg > 1:2271962/1‑62 (MQ=255) taatCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGaa < 1:3514358/51‑1 (MQ=255) taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAcgcg < 1:3651241/71‑1 (MQ=255) aatCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGtt > 1:1424923/1‑54 (MQ=255) atCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtg > 1:4497600/1‑43 (MQ=255) tgCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAg < 1:3448480/69‑1 (MQ=255) tgCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCg > 1:375101/1‑71 (MQ=255) tgCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCg < 1:3077347/71‑1 (MQ=255) gcgcGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGttt < 1:2037544/48‑1 (MQ=255) cgcgGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTg > 1:4487220/1‑71 (MQ=255) gcgGTGAATATGCCCGAGCGCGATGTGGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGc < 1:2855936/71‑1 (MQ=255) gcgGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGc > 1:2505367/1‑71 (MQ=255) cgGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGc > 1:4372218/1‑70 (MQ=255) cgGTGAATATGCCCGAGCGCGATGTAATCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGcc < 1:1695029/71‑1 (MQ=255) tGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCaa > 1:478308/1‑70 (MQ=255) tGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:2906398/41‑1 (MQ=255) aaTATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat < 1:2788139/71‑1 (MQ=255) aaTATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtttt > 1:4235935/1‑41 (MQ=255) atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat > 1:3660931/1‑70 (MQ=255) atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat > 1:85986/1‑70 (MQ=255) atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:4157659/71‑1 (MQ=255) atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:2197829/71‑1 (MQ=255) atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:537717/71‑1 (MQ=255) atatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat > 1:161868/1‑70 (MQ=255) tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGc > 1:2128687/1‑43 (MQ=255) tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCa < 1:565045/65‑1 (MQ=255) tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:941429/70‑1 (MQ=255) tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATaa < 1:166549/71‑1 (MQ=255) tGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:4423294/68‑1 (MQ=255) gCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATaa > 1:3841255/1‑68 (MQ=255) gCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATaa > 1:546742/1‑68 (MQ=255) cccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATg < 1:3875163/70‑1 (MQ=255) cccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGt > 1:329979/1‑71 (MQ=255) ccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGcc > 1:1801636/1‑39 (MQ=255) cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGa < 1:2650914/41‑1 (MQ=255) cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCa < 1:1424634/59‑1 (MQ=255) cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCa < 1:1111497/59‑1 (MQ=255) | ATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGT > minE/244697‑244829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |