| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 253,106 | C→G | 6.7% | P94P (CCG→CCC) | yajB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 253,106 | 0 | C | G | 6.7% | 212.9 / 2.9 | 74 | P94P (CCG→CCC) | yajB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (31/38); new base G (2/3); total (33/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAAC > minE/253037‑253174 | cGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCg < 1:3205937/71‑1 (MQ=255) tGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGc > 1:785259/1‑70 (MQ=255) gAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGa > 1:963467/1‑71 (MQ=255) ccGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGc > 1:1116653/1‑71 (MQ=255) cGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAg > 1:595818/1‑69 (MQ=255) cGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCt > 1:4465088/1‑71 (MQ=255) ggCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCt < 1:3148291/70‑1 (MQ=255) gCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGc > 1:2770646/1‑71 (MQ=255) gCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGc < 1:2015330/71‑1 (MQ=255) cAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGa > 1:1567038/1‑63 (MQ=255) aaaaTCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGa > 1:4203690/1‑71 (MQ=255) aaaaTCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGa > 1:351072/1‑71 (MQ=255) aaTCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGTAAAGTCCGGCGACAGCTGCGAcc < 1:1412009/71‑1 (MQ=255) aaTCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTcc < 1:875340/55‑1 (MQ=255) aTCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGAc < 1:3090839/60‑1 (MQ=255) gTCATCACTTGCTCGCGGGCATAACAGACGAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGa > 1:1961871/1‑65 (MQ=255) atcaCTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCg > 1:2715597/1‑71 (MQ=255) atcaCTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCg > 1:924703/1‑71 (MQ=255) atcaCTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCg > 1:1220390/1‑71 (MQ=255) aCTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACa > 1:4064456/1‑52 (MQ=255) aCTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCggg > 1:1631821/1‑70 (MQ=255) cTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGc < 1:771913/47‑1 (MQ=255) tGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGa > 1:519618/1‑47 (MQ=255) gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGtaaag < 1:719261/70‑1 (MQ=255) gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaag > 1:3901398/1‑70 (MQ=255) gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaag > 1:2865832/1‑70 (MQ=255) gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaag > 1:4614777/1‑70 (MQ=255) cTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaaga < 1:2626579/70‑1 (MQ=255) cTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaaga < 1:4108210/70‑1 (MQ=255) cTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaaga < 1:3089030/70‑1 (MQ=255) gcgGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGaaagaaag < 1:866931/70‑1 (MQ=255) cgGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTg < 1:4687668/71‑1 (MQ=255) gCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCggg < 1:704097/58‑1 (MQ=255) tAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaagaa < 1:2747707/61‑1 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACa > 1:434620/1‑35 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCa > 1:350263/1‑45 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcaca > 1:2702795/1‑71 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcaca > 1:430510/1‑71 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcaca > 1:212789/1‑71 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcac < 1:315668/70‑1 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcac < 1:1484874/70‑1 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcac < 1:1033690/70‑1 (MQ=255) cAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACAt < 1:99663/71‑1 (MQ=255) aGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATc > 1:1689288/1‑64 (MQ=255) gACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGAc < 1:1697726/40‑1 (MQ=255) gACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCca > 1:4552861/1‑66 (MQ=255) gACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGa > 1:4604117/1‑71 (MQ=255) cAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACa > 1:4022855/1‑71 (MQ=255) aaaTTCCTGTAGCGGAAAGTCCGGCGACAGCTGAGAcc < 1:2095636/38‑1 (MQ=255) aaTTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaagaaa < 1:1869637/53‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGaaagaaag < 1:3250545/46‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1151928/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1391312/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1820171/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1903508/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:4225871/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:2980141/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:3739492/71‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:1838916/1‑71 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:25562/1‑69 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATa < 1:1471233/71‑1 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATa < 1:3161041/71‑1 (MQ=255) cGGAAAGTCCGGCGACAGCTGCGACCAGTGGCggg < 1:2486253/35‑1 (MQ=255) ggAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAgg > 1:3452352/1‑71 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:53627/63‑1 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:3590256/63‑1 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:840047/63‑1 (MQ=255) aaaaTCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGgcg > 1:3221462/1‑71 (MQ=255) aaaaTCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGgcg > 1:1193590/1‑71 (MQ=255) aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:2964105/63‑1 (MQ=255) aaGTCCGTCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGgcgc < 1:452907/71‑1 (MQ=255) aaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGgcgc < 1:3661874/71‑1 (MQ=255) gTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:1387258/62‑1 (MQ=255) tCCGGCGACAGCTGCGTCCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAAc < 1:4210695/71‑1 (MQ=255) tCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:2923251/61‑1 (MQ=255) ccGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACAt > 1:336821/1‑46 (MQ=255) cGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATcc > 1:1792563/1‑40 (MQ=255) | CGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAAC > minE/253037‑253174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |