| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 253,133 | A→C | 10.0% | L85L (CTT→CTG) | yajB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 253,133 | 0 | A | C | 10.0% | 128.8 / 12.6 | 60 | L85L (CTT→CTG) | yajB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (18/36); new base C (2/4); total (20/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
GCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAC > minE/253066‑253196 | gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGtaaag < 1:719261/70‑1 (MQ=255)gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaag > 1:4614777/1‑70 (MQ=255)gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaag > 1:2865832/1‑70 (MQ=255)gCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaag > 1:3901398/1‑70 (MQ=255) cTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaaga < 1:2626579/70‑1 (MQ=255) cTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaaga < 1:3089030/70‑1 (MQ=255) cTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaaga < 1:4108210/70‑1 (MQ=255) gcgGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGaaagaaag < 1:866931/70‑1 (MQ=255) cgGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTg < 1:4687668/71‑1 (MQ=255) tAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaagaa < 1:2747707/61‑1 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcaca > 1:2702795/1‑71 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcaca > 1:212789/1‑71 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcaca > 1:430510/1‑71 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcac < 1:315668/70‑1 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcac < 1:1484874/70‑1 (MQ=255) aCAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCcac < 1:1033690/70‑1 (MQ=255) cAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACAt < 1:99663/71‑1 (MQ=255) aGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATc > 1:1689288/1‑64 (MQ=255) gACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCca > 1:4552861/1‑66 (MQ=255) gACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGa > 1:4604117/1‑71 (MQ=255) cAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACa > 1:4022855/1‑71 (MQ=255) aaTTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaagaaa < 1:1869637/53‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGaaagaaag < 1:3250545/46‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:4225871/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:2980141/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1151928/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1820171/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1903508/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1391312/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:1838916/1‑71 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:3739492/71‑1 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:25562/1‑69 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATa < 1:3161041/71‑1 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATa < 1:1471233/71‑1 (MQ=255) ggAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAgg > 1:3452352/1‑71 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:840047/63‑1 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:3590256/63‑1 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:53627/63‑1 (MQ=255) aaaaTCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGgcg > 1:3221462/1‑71 (MQ=255) aaaaTCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGgcg > 1:1193590/1‑71 (MQ=255) aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:2964105/63‑1 (MQ=255) aaGTCCGTCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGgcgc < 1:452907/71‑1 (MQ=255) aaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGgcgc < 1:3661874/71‑1 (MQ=255) gTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:1387258/62‑1 (MQ=255) tCCGGCGACAGCTGCGTCCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAAc < 1:4210695/71‑1 (MQ=255) tCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:2923251/61‑1 (MQ=255) ccGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACAt > 1:336821/1‑46 (MQ=255) gggCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGGCGCGAcac < 1:538874/70‑3 (MQ=255) cGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATcc > 1:1792563/1‑40 (MQ=255) ggCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCca < 1:2553607/41‑1 (MQ=255) aGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCaa < 1:4159342/61‑1 (MQ=255) gCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:4645886/49‑1 (MQ=255) cTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGttt < 1:4619550/71‑1 (MQ=255) cTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGttt < 1:1920091/71‑1 (MQ=255) gCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTc > 1:1516869/1‑70 (MQ=255) gACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCAc > 1:753007/1‑70 (MQ=255) gACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACt < 1:3199801/71‑1 (MQ=255) aCCAGTGGCGGGAAAGAAAGTGATCCCACATGACATc < 1:802504/37‑1 (MQ=255) cAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:3180278/41‑1 (MQ=255) aGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCTTAATAGGCGCAACGCGGCGCGTTTCACTACGa < 1:2282900/71‑1 (MQ=255) aGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:818307/1‑42 (MQ=255) aGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCaa < 1:1233666/51‑1 (MQ=255) tGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:422384/40‑1 (MQ=255) tGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:4292858/40‑1 (MQ=255) tGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACt < 1:3164160/65‑1 (MQ=255) gCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:1575056/1‑38 (MQ=255) gCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc > 1:655327/1‑70 (MQ=255) gggAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:4333926/1‑36 (MQ=255) ggAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:606373/35‑1 (MQ=255) ggAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:1415144/35‑1 (MQ=255) ggAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc < 1:452597/67‑1 (MQ=255) aaagaaagTGATCCCACATGACATCCAGCGTAATAGGCGCa > 1:2959334/1‑41 (MQ=255) aagaaagTGATCCCACATGACATCCAGCGTAATAGGCGCaa > 1:1396796/1‑41 (MQ=255) aagaaagTGATCCCACATGACATCCAGCGTAATAGGCGCaa > 1:1868362/1‑41 (MQ=255) | GCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAC > minE/253066‑253196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |