| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 253,151 | G→C | 8.5% | V79V (GTC→GTG) | yajB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 253,151 | 0 | G | C | 8.5% | 193.4 / 6.7 | 70 | V79V (GTC→GTG) | yajB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (25/39); new base C (2/4); total (27/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCC > minE/253082‑253220 | gACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGa > 1:4604117/1‑71 (MQ=255) cAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACa > 1:4022855/1‑71 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1151928/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1820171/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1903508/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:2980141/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:1391312/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:4225871/69‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:3739492/71‑1 (MQ=255) tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:1838916/1‑71 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:25562/1‑69 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATa < 1:3161041/71‑1 (MQ=255) gCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATa < 1:1471233/71‑1 (MQ=255) ggAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAgg > 1:3452352/1‑71 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:3590256/63‑1 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:53627/63‑1 (MQ=255) gAAAATCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCg < 1:840047/63‑1 (MQ=255) aaaaTCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGgcg > 1:1193590/1‑71 (MQ=255) aaaaTCGGGCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGgcg > 1:3221462/1‑71 (MQ=255) aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:2964105/63‑1 (MQ=255) aaGTCCGTCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGgcgc < 1:452907/71‑1 (MQ=255) aaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGgcgc < 1:3661874/71‑1 (MQ=255) gTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:1387258/62‑1 (MQ=255) tCCGGCGACAGCTGCGTCCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAAc < 1:4210695/71‑1 (MQ=255) tCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:2923251/61‑1 (MQ=255) gggCGACAGCTGCGACCAGTGGCGGGACAGAAAGTGATCCCACATCACATCCAGCGTAATAGGCGCGAcac < 1:538874/70‑3 (MQ=255) aGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCaa < 1:4159342/61‑1 (MQ=255) gCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:4645886/49‑1 (MQ=255) cTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGttt < 1:4619550/71‑1 (MQ=255) cTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGttt < 1:1920091/71‑1 (MQ=255) gCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTc > 1:1516869/1‑70 (MQ=255) gACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCAc > 1:753007/1‑70 (MQ=255) gACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACt < 1:3199801/71‑1 (MQ=255) aCCAGTGGCGGGAAAGAAAGTGATCCCACATGACATc < 1:802504/37‑1 (MQ=255) cAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGt < 1:3180278/41‑1 (MQ=255) aGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCTTAATAGGCGCAACGCGGCGCGTTTCACTACGa < 1:2282900/71‑1 (MQ=255) aGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:818307/1‑42 (MQ=255) aGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCaa < 1:1233666/51‑1 (MQ=255) tGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:4292858/40‑1 (MQ=255) tGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:422384/40‑1 (MQ=255) tGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACt < 1:3164160/65‑1 (MQ=255) gCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:1575056/1‑38 (MQ=255) gCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc > 1:655327/1‑70 (MQ=255) gggAAAGAAAGTGATCCCACATGACATCCAGCGTaa > 1:4333926/1‑36 (MQ=255) ggAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:1415144/35‑1 (MQ=255) ggAAAGAAAGTGATCCCACATGACATCCAGCGTaa < 1:606373/35‑1 (MQ=255) ggAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc < 1:452597/67‑1 (MQ=255) aaagaaagTGATCCCACATGACATCCAGCGTAATAGGCGCa > 1:2959334/1‑41 (MQ=255) aagaaagTGATCCCACATGACATCCAGCGTAATAGGCGCaa > 1:1868362/1‑41 (MQ=255) aagaaagTGATCCCACATGACATCCAGCGTAATAGGCGCaa > 1:1396796/1‑41 (MQ=255) aaagTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTg > 1:4596237/1‑71 (MQ=255) aagTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGc > 1:1506839/1‑71 (MQ=255) tGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGcgcg < 1:3150599/43‑1 (MQ=255) tGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTc < 1:3481779/71‑1 (MQ=255) gATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTcg > 1:4550452/1‑71 (MQ=255) gATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTcg > 1:3776019/1‑71 (MQ=255) aTCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTcg < 1:120503/70‑1 (MQ=255) cccACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGa > 1:1156791/1‑50 (MQ=255) cacaTGACATCCAGCGTAATAGGCGCAACGCGGCGCGttt < 1:2514798/40‑1 (MQ=255) cacaTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGa < 1:1426850/48‑1 (MQ=255) acaTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCAc > 1:2768526/1‑53 (MQ=255) acaTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACt < 1:1434039/54‑1 (MQ=255) caTGACATCCAGCGTAATAGGCGCAACGCGGCGCGttt < 1:1190372/38‑1 (MQ=255) caTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACtt < 1:1162786/71‑1 (MQ=255) caTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACtt < 1:4151564/71‑1 (MQ=255) aTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCa > 1:3428453/1‑39 (MQ=255) aTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGaaa > 1:4413632/1‑47 (MQ=255) aTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTc > 1:3142482/1‑71 (MQ=255) aTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTc > 1:1081955/1‑71 (MQ=255) tGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTcc > 1:4663208/1‑71 (MQ=255) | GACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCC > minE/253082‑253220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |