| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 316,095 | A→G | 64.2% | N364S (AAC→AGC) | kefA → | fused mechanosensitive channel proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 316,095 | 0 | A | G | 64.2% | 129.1 / 118.4 | 123 | N364S (AAC→AGC) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base A (21/23); new base G (38/41); total (59/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
GGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCT > minE/316025‑316163 | gCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGaaaa > 1:1313001/1‑70 (MQ=255) cAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCa > 1:3567170/1‑71 (MQ=255) cAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCa < 1:3853223/71‑1 (MQ=255) aGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCAt < 1:648954/71‑1 (MQ=255) aGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACAt < 1:1225611/71‑1 (MQ=255) aGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACAt > 1:76848/1‑71 (MQ=255) ctgTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCaa > 1:1487130/1‑71 (MQ=255) ctgTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCa < 1:1721958/70‑1 (MQ=255) ctgTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCaa > 1:4059004/1‑71 (MQ=255) tctcGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCa < 1:3217399/70‑1 (MQ=255) tcGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCa < 1:3907527/62‑1 (MQ=255) tcGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATc > 1:881836/1‑70 (MQ=255) gTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGa > 1:882087/1‑57 (MQ=255) gTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATcgcg > 1:30408/1‑71 (MQ=255) gTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATcgcg > 1:3423386/1‑71 (MQ=255) aTCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGa > 1:2863600/1‑71 (MQ=255) tCCTTTACCAGCAACAACATACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGAt > 1:734104/1‑71 (MQ=255) tCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGTAAAGCATGACCAACCGCATCGCGGa < 1:1534478/70‑1 (MQ=255) tCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGAt > 1:137502/1‑71 (MQ=255) ccTTTACCAGCAACGACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCAt < 1:1518134/51‑1 (MQ=39) ccTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCAt < 1:1819054/51‑1 (MQ=255) cTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGAttt > 1:3057854/1‑71 (MQ=255) cTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGAttt < 1:280977/71‑1 (MQ=255) cTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGAtt > 1:2762042/1‑70 (MQ=255) ttACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGc > 1:1303080/1‑71 (MQ=255) ttACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATcgc > 1:3844424/1‑63 (MQ=255) tACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCgg < 1:3290451/64‑1 (MQ=255) tACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGc < 1:2541866/70‑1 (MQ=255) tACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCg > 1:2939878/1‑71 (MQ=255) aCCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCa > 1:565092/1‑45 (MQ=255) aCCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCg < 1:783370/70‑1 (MQ=255) ccAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGtc < 1:3382205/71‑1 (MQ=255) ccAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGtc < 1:4291750/71‑1 (MQ=255) aGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACAt < 1:4331295/43‑1 (MQ=255) gCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAAc < 1:3213820/40‑1 (MQ=255) gCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCg < 1:2292388/71‑1 (MQ=255) caacaacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCaa > 1:4649372/1‑47 (MQ=255) caacaacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCg > 1:2944986/1‑70 (MQ=255) acaacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGc < 1:3366840/49‑1 (MQ=255) acaacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATc > 1:2089822/1‑52 (MQ=255) caacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCg < 1:281242/67‑1 (MQ=255) caacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCg < 1:579565/67‑1 (MQ=255) caacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAAc < 1:2682893/70‑1 (MQ=255) caacaaACGCTGCCCTCGGCGGATGAACTGGAAAACAt < 1:1871919/38‑1 (MQ=255) aacaaACGCTTCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGa < 1:338840/55‑1 (MQ=255) aacaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGa < 1:3026114/55‑1 (MQ=255) aacaaACGCTGCCCTCGGCGGATGAACTGGAAAACAt > 1:476461/1‑37 (MQ=255) aacaaACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACat > 1:117850/1‑70 (MQ=255) aacaaACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAg > 1:911349/1‑71 (MQ=255) acaaACGCTGCCCTCGGCGGATGAACTGGAAAGCATg > 1:2040273/1‑37 (MQ=255) caaACGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCa < 1:2613610/40‑1 (MQ=255) caaACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGtt < 1:4497018/71‑1 (MQ=255) aaaCGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGt > 1:1607362/1‑69 (MQ=255) aaCGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTg < 1:3649958/71‑1 (MQ=255) aCGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGa < 1:2094419/71‑1 (MQ=255) aCGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGa < 1:4478502/71‑1 (MQ=255) aCGCTGCCCTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGa < 1:4011523/71‑1 (MQ=255) cccTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTaa < 1:3902306/71‑1 (MQ=255) ccTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAc < 1:1366847/71‑1 (MQ=255) ccTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAc < 1:4079431/71‑1 (MQ=255) cTCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGa < 1:1807890/42‑1 (MQ=255) cTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAcc < 1:3451217/71‑1 (MQ=255) tCGGCGGATGAACTGGAAAGCATGACCAACCGCATCGCGGAtt < 1:2415291/43‑1 (MQ=255) tCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAg < 1:2181028/57‑1 (MQ=255) tCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAg < 1:519648/57‑1 (MQ=255) tCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTg < 1:2760275/61‑1 (MQ=255) tCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAc > 1:2029563/1‑69 (MQ=255) cggcggATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACcag > 1:445645/1‑71 (MQ=255) ggcggATGAACTGGAAAGCATGACCAACCGCATCGCGGAtt > 1:3703121/1‑41 (MQ=255) ggcggATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAc > 1:1354404/1‑67 (MQ=255) ggcggATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACcagc < 1:4204648/71‑1 (MQ=255) ggcggATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACcag > 1:2151783/1‑70 (MQ=255) ggcggATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACcag > 1:1409215/1‑70 (MQ=255) gcggATGAACTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACcag > 1:1209202/1‑69 (MQ=255) ggATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCagcagc < 1:3993147/71‑1 (MQ=255) aTGAACTTGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGt < 1:4558351/71‑1 (MQ=255) aTGAACTTGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGt < 1:1425328/71‑1 (MQ=255) tGAACTTGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGaa > 1:518977/1‑55 (MQ=255) tGAACTGGAAAGCATGACCAACCGCATCGCGGAtt > 1:1790796/1‑35 (MQ=255) tGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTg < 1:53070/71‑1 (MQ=255) gAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAcc > 1:2111505/1‑61 (MQ=255) aaCTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGttt < 1:1185293/50‑1 (MQ=255) aaCTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGAt > 1:2348021/1‑71 (MQ=255) aCTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGt < 1:4035004/67‑1 (MQ=255) aCTGGAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATg < 1:2968208/71‑1 (MQ=255) aCTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAc > 1:496120/1‑58 (MQ=255) aCTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATg < 1:4393004/71‑1 (MQ=255) aCTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATg < 1:2905319/71‑1 (MQ=255) cTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATg > 1:273161/1‑70 (MQ=255) tGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGaa > 1:2526725/1‑50 (MQ=255) tGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCa > 1:539552/1‑71 (MQ=255) ggAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGtt < 1:4219195/52‑1 (MQ=255) ggAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGa > 1:772507/1‑66 (MQ=255) ggAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCAc > 1:3904842/1‑71 (MQ=255) gAAAGCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAGCAGCAGCGTGATGCAct > 1:1805076/1‑71 (MQ=255) gAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGa > 1:3439494/1‑47 (MQ=255) gAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAAcc > 1:1675295/1‑55 (MQ=255) gAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCAct < 1:3184427/71‑1 (MQ=255) gAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCAct > 1:4093399/1‑71 (MQ=255) aaCATGACCAACCGCATCGCGGATTTGCGTCTCGAACAg < 1:2404638/39‑1 (MQ=255) | GGCAGCCTGCTGTTGTCTCGTATCCTTTACCAGCAACAACAAACGCTGCCCTCGGCGGATGAACTGGAAAACATGACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCT > minE/316025‑316163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |