| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 379,337 | A→G | 63.9% | V24A (GTT→GCT) | fepB ← | iron‑enterobactin transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 379,337 | 0 | A | G | 63.9% | 61.1 / 55.5 | 61 | V24A (GTT→GCT) | fepB | iron‑enterobactin transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (5/17); new base G (17/22); total (22/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCTGGCTTTCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCA > minE/379270‑379406 | cGGCTGGCTTTCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCt < 1:1708423/70‑1 (MQ=255) ctggctTTCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTg < 1:177603/68‑1 (MQ=255) gctTTCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGc < 1:3705914/69‑1 (MQ=255) gctTTCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCt < 1:889018/70‑1 (MQ=255) tCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTAtt < 1:3583510/69‑1 (MQ=255) tCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTcc > 1:491600/1‑71 (MQ=255) tgtATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGa < 1:2961034/69‑1 (MQ=255) tATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAg > 1:1208136/1‑70 (MQ=255) tgtgtgCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCaa < 1:2734376/71‑1 (MQ=255) tgtgtgCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCaa < 1:1933877/71‑1 (MQ=255) tgtgtgCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCa < 1:2615257/70‑1 (MQ=255) tgtgtgCCACGGCTGTCAGTAATCTGACGCGGCCAGTAAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCa > 1:3855081/1‑70 (MQ=255) tgtgCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCaaaa < 1:2673455/71‑1 (MQ=255) tgtgCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTcc < 1:4057626/60‑1 (MQ=255) tgtgCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTcc < 1:684474/60‑1 (MQ=255) ccACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCaaaa > 1:340777/1‑67 (MQ=255) ccACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTc > 1:4358973/1‑70 (MQ=255) cACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCt > 1:2959397/1‑71 (MQ=255) aCGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTg < 1:1956813/71‑1 (MQ=255) ggCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGtt < 1:1872098/71‑1 (MQ=255) ggCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGtt < 1:3203485/71‑1 (MQ=255) ggCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGt > 1:2996739/1‑70 (MQ=255) ggCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGtt < 1:2898766/71‑1 (MQ=255) tCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATa < 1:3303396/70‑1 (MQ=255) tCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCt < 1:662835/63‑1 (MQ=255) tCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAg < 1:4502131/71‑1 (MQ=255) aGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAg > 1:3550602/1‑69 (MQ=255) aGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGaa < 1:3173848/71‑1 (MQ=255) aGTAATCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGaa < 1:187181/71‑1 (MQ=255) aGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAg > 1:814149/1‑69 (MQ=255) aGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAg > 1:1032558/1‑69 (MQ=255) aGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGa < 1:1230193/70‑1 (MQ=255) aGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGa < 1:3147529/70‑1 (MQ=255) aTCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAggg < 1:314731/70‑1 (MQ=255) aTCTGACGCGGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGc > 1:1890712/1‑71 (MQ=255) cgcgGCCAGTCAGAGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCg > 1:3657211/1‑71 (MQ=255) gcgGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCgg < 1:882741/71‑1 (MQ=255) cgGCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCaa < 1:266538/42‑1 (MQ=255) ggCCAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAg < 1:3200664/56‑1 (MQ=255) ggCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGt < 1:4409131/50‑1 (MQ=255) ccAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCaa < 1:1066245/39‑1 (MQ=255) cAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAg < 1:4337081/56‑1 (MQ=255) cAGTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTag > 1:680094/1‑69 (MQ=255) gTCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTaa < 1:2529581/48‑1 (MQ=255) tCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCaa < 1:2697974/35‑1 (MQ=255) tCAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGtt < 1:3721760/45‑1 (MQ=255) tCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCaa < 1:1787638/35‑1 (MQ=255) cAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATa > 1:2951213/1‑48 (MQ=255) cAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAg < 1:2844957/49‑1 (MQ=255) cAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCggg > 1:3807870/1‑71 (MQ=255) cAGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCggg > 1:327140/1‑71 (MQ=255) aGCGGCCTGAGCTGCGGCTATTCCTGAAAGCaaaa > 1:4487705/1‑35 (MQ=255) aGCGGCCTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCg > 1:272489/1‑55 (MQ=255) aGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCgggg > 1:1418299/1‑71 (MQ=255) gCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGc < 1:2165633/71‑1 (MQ=255) gCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGtt < 1:3468339/39‑1 (MQ=255) ccTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATa < 1:1939636/42‑1 (MQ=255) cTGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAg > 1:364784/1‑69 (MQ=255) cTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTaa > 1:412212/1‑39 (MQ=255) tGAGCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGt < 1:560093/55‑1 (MQ=255) tGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTaa > 1:1007040/1‑38 (MQ=255) aaCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTaga > 1:3625871/1‑56 (MQ=255) aaCTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTca > 1:2715094/1‑71 (MQ=255) | CGGCTGGCTTTCCAGTGTATGTGTGCCACGGCTGTCAGTAATCTGACGCGGCCAGTCAGCGGCCTGAACTGCGGCTATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCA > minE/379270‑379406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |