| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 391,026 | T→C | 5.6% | V147V (GTA→GTG) | ybdN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 391,026 | 0 | T | C | 5.6% | 345.4 / 9.1 | 127 | V147V (GTA→GTG) | ybdN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (63/56); new base C (3/4); total (67/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
CTCATCCGCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCTTGGG > minE/390957‑391096 | cTCATCCGCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTa > 1:3969277/1‑71 (MQ=255) tCATCCGCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTa > 1:1044648/1‑70 (MQ=255) aTCCGCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACa > 1:1356012/1‑70 (MQ=255) aTCCGCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACa > 1:325114/1‑70 (MQ=255) aTCCACACGGATGCCGATCATCATAGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACa > 1:881061/1‑70 (MQ=255) ccGCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAAt > 1:318522/1‑71 (MQ=255) cGCACGGATGCCGATCATCATCGCCGCAGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAAt > 1:501921/1‑70 (MQ=255) cGCACGGAAGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAAtt > 1:2285605/1‑71 (MQ=255) gCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTg > 1:3206433/1‑71 (MQ=255) cACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTg < 1:3086148/70‑1 (MQ=255) aCGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGtt < 1:1784229/71‑1 (MQ=255) ggATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACTAATTGTTCg < 1:3893955/71‑1 (MQ=255) gATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGa < 1:2793051/71‑1 (MQ=255) gATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGa < 1:2413708/71‑1 (MQ=255) gATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGa < 1:234544/71‑1 (MQ=255) gATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCAGCAAACTCACGCACAAATTGTTCGa < 1:287684/71‑1 (MQ=255) tGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCg > 1:1266523/1‑68 (MQ=255) ccGATCGTCATCGCCGCCGGACGTTTTTGTGAAAACAATTCGGCAAACTCACGTACAAATTGTTCGAAGGt < 1:4640012/71‑1 (MQ=255) ccGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGt > 1:1127536/1‑71 (MQ=255) cGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACa < 1:2820959/56‑1 (MQ=255) cGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAgg < 1:1502945/69‑1 (MQ=255) cGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTc < 1:1148889/71‑1 (MQ=255) gATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGt < 1:1245595/69‑1 (MQ=255) gATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGt < 1:3985933/69‑1 (MQ=255) gATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGt < 1:185541/69‑1 (MQ=255) gATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCa < 1:174939/71‑1 (MQ=255) tcatcatcGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATg < 1:1335099/71‑1 (MQ=255) catcatcGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGtt > 1:2439000/1‑59 (MQ=255) catcatcGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGc > 1:2766298/1‑71 (MQ=255) tcatcGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTg < 1:1602040/55‑1 (MQ=255) atcGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGAACAAATTg > 1:1517372/1‑53 (MQ=255) atcGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACACACGTACAAATTGtt > 1:1712466/1‑55 (MQ=255) tcGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCctggc > 1:1540735/1‑71 (MQ=255) gccgccGTACGTTTTTGTGAAAAACATTCGGCAAACTCACGTACAAATTGTTCGaa > 1:3395678/1‑56 (MQ=255) gccgccGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGt > 1:2990410/1‑51 (MQ=255) gccgccGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAg > 1:850137/1‑57 (MQ=255) gccgccGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCa > 1:1594875/1‑61 (MQ=255) ccgGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTaa < 1:1381741/71‑1 (MQ=255) cgGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTaaa < 1:3869196/71‑1 (MQ=255) gACGTTTTTGTGAAAACCATTCGGCAAACTCACGTAc < 1:3373679/37‑1 (MQ=255) gACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAg > 1:4184085/1‑71 (MQ=255) gACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAg > 1:837243/1‑71 (MQ=255) cGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAg > 1:3530021/1‑69 (MQ=255) gTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCg < 1:104011/44‑1 (MQ=255) tttttGTGAAAACCATTCGGCAAACTCACGTACAAAt < 1:4504369/37‑1 (MQ=255) tttttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGt < 1:3813957/48‑1 (MQ=255) tttttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCAt < 1:3400153/51‑1 (MQ=255) tttttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCAt < 1:1880885/51‑1 (MQ=255) ttttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGc > 1:3969405/1‑52 (MQ=255) ttttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGc > 1:3491629/1‑52 (MQ=255) ttttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGa > 1:4068434/1‑70 (MQ=255) ttttGTGAAAACCATTCAGCAAACTCACGCACAAATTGTTCGAAGGTCATGCCAGGCTGGTAAAAGgaga > 1:1867742/1‑70 (MQ=255) tttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTaaa > 1:4416201/1‑63 (MQ=255) tttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGaa > 1:2571448/1‑70 (MQ=255) tttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGaa > 1:1626304/1‑70 (MQ=255) tttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGaa > 1:1972868/1‑70 (MQ=255) tttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGaa > 1:2200485/1‑70 (MQ=255) tttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGaa > 1:294152/1‑70 (MQ=255) ttGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGaaa < 1:612414/70‑1 (MQ=255) gtgAAAACCATTCGGCAAACTCACGTACAAATTGTTCGaa > 1:3286397/1‑40 (MQ=255) gtgAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGt < 1:1752750/43‑1 (MQ=255) tgAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCa > 1:4178832/1‑44 (MQ=255) gAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTc > 1:101393/1‑42 (MQ=255) aaaaCCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGt > 1:1942881/1‑69 (MQ=255) aaaCCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTAAg < 1:755849/71‑1 (MQ=255) aaCCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCa > 1:531994/1‑40 (MQ=255) aaCCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAgg < 1:3925017/71‑1 (MQ=255) aCCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCATGGCTGGTAAAAGCAGAAAAAGTCa < 1:1454557/68‑1 (MQ=255) ttCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTaaaa < 1:4658131/51‑1 (MQ=255) ttCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAg < 1:3435100/52‑1 (MQ=255) ttCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCgg > 1:4041303/1‑71 (MQ=255) cGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCgg > 1:3051526/1‑69 (MQ=255) cGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGt < 1:4602312/70‑1 (MQ=255) cGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGt > 1:815986/1‑70 (MQ=255) cGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGt > 1:3038976/1‑70 (MQ=255) ggCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTa < 1:3088031/71‑1 (MQ=255) gCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCg < 1:3579646/66‑1 (MQ=255) gCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCg < 1:2880610/66‑1 (MQ=255) cAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATc < 1:2051877/71‑1 (MQ=255) aaaCTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCg < 1:2687481/71‑1 (MQ=255) aaCTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGaaaaa > 1:665236/1‑53 (MQ=255) aaCTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGc < 1:331272/71‑1 (MQ=255) aaCTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGc < 1:1146919/71‑1 (MQ=255) aCTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCa > 1:1469720/1‑56 (MQ=255) aCTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCa > 1:4042166/1‑71 (MQ=255) aCTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCa > 1:45172/1‑71 (MQ=255) aCTCACGCACAAATTGTTCGAAGGTCATGCCAGGCTGGTAAAAGGAGAAAAAGTCAGGATCGGTTATCGCa > 1:693482/1‑71 (MQ=255) cTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAgg > 1:3241197/1‑57 (MQ=255) cTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAAGGTCGGTTATCGCAt < 1:3317108/71‑1 (MQ=255) cTCACGCACAAATTGTTCGAAGGTCATGCCAGGCTGGTAAAAGGAGAAAAAGTCAGGATCGGTTATCGCAt < 1:216659/71‑1 (MQ=255) tCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGt < 1:3146652/37‑1 (MQ=255) aCGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGc > 1:1975871/1‑41 (MQ=255) aCGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCgcta > 1:2576764/1‑58 (MQ=255) aCGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCtt > 1:1043295/1‑71 (MQ=255) aCGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCt < 1:2968578/70‑1 (MQ=255) cGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCtt < 1:1252938/70‑1 (MQ=255) cGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCTTg > 1:1423174/1‑71 (MQ=255) tACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAg < 1:912499/40‑1 (MQ=255) tACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCTTggg < 1:4204100/71‑1 (MQ=255) tACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCTTggg < 1:865617/71‑1 (MQ=255) | CTCATCCGCACGGATGCCGATCATCATCGCCGCCGGACGTTTTTGTGAAAACCATTCGGCAAACTCACGTACAAATTGTTCGAAGGTCATGCCTGGCTGGTAAAAGCAGAAAAAGTCAGGGTCGGTTATCGCATCTTGGG > minE/390957‑391096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |