| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 497,847 | A→G | 61.1% | Y222H (TAT→CAT) | galE ← | UDP‑galactose‑4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 497,847 | 0 | A | G | 61.1% | 106.1 / 139.6 | 131 | Y222H (TAT→CAT) | galE | UDP‑galactose‑4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (24/27); new base G (26/54); total (50/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
ACGACGTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGT > minE/497777‑497915 | acgacgTTACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATa < 1:1731217/71‑1 (MQ=255)acgacgTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATa < 1:4469401/71‑1 (MQ=255) gacgTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGgatgat > 1:3002866/1‑71 (MQ=255) tGACCGTCCGCCTGATCCATTTCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGtt < 1:4537535/71‑1 (MQ=255) aCCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTAc > 1:1002372/1‑71 (MQ=255) aCCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATAGTTAc > 1:2207481/1‑71 (MQ=255) aCCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTAc > 1:3295625/1‑71 (MQ=255) ccGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTAcc < 1:773555/71‑1 (MQ=255) cGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCg < 1:2347635/65‑1 (MQ=255) tCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTTGGATGATCGTTACCaaa < 1:1751842/71‑1 (MQ=255) tCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCaaa < 1:1634269/71‑1 (MQ=255) cGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa < 1:4645788/70‑1 (MQ=255) cGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa < 1:2915562/70‑1 (MQ=255) cAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCg > 1:4193978/1‑71 (MQ=255) gATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCaaaa < 1:944947/65‑1 (MQ=255) ccATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCTGATGATCGTTACCAAAAATCGCCAGc > 1:2538671/1‑71 (MQ=255) ttACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAg < 1:1665154/71‑1 (MQ=255) ttACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAg < 1:3195512/71‑1 (MQ=255) ttACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAg < 1:4505300/71‑1 (MQ=255) atACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAg < 1:211748/70‑1 (MQ=255) aCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc > 1:3869486/1‑71 (MQ=255) aCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc > 1:1435776/1‑71 (MQ=255) aCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTc > 1:3875004/1‑71 (MQ=255) gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTcgc < 1:668230/71‑1 (MQ=255) gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTcgc > 1:3918842/1‑71 (MQ=255) gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTcgc > 1:1567558/1‑71 (MQ=255) gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTcgc < 1:130795/71‑1 (MQ=255) aTGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACg < 1:2120846/71‑1 (MQ=255) tGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTcgc < 1:1991597/66‑1 (MQ=255) tGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACgg < 1:3729872/71‑1 (MQ=255) tGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACgg < 1:3089/71‑1 (MQ=255) tAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGc < 1:2148834/57‑1 (MQ=255) tAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGc > 1:3014983/1‑70 (MQ=255) aaTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGt > 1:1966403/1‑39 (MQ=255) aaTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGc < 1:1301696/69‑1 (MQ=255) aaTCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc > 1:613053/1‑70 (MQ=255) aaTCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc > 1:377927/1‑70 (MQ=255) aTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCt < 1:425898/70‑1 (MQ=255) aTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTa > 1:17805/1‑71 (MQ=255) aTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTa < 1:1125128/71‑1 (MQ=255) gcgTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACa < 1:3563568/70‑1 (MQ=255) gcgTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAg > 1:4229149/1‑71 (MQ=255) gcgTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAg > 1:1750080/1‑71 (MQ=255) gTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa < 1:3880190/41‑1 (MQ=255) gTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa < 1:4002483/41‑1 (MQ=255) tACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATc < 1:3551000/43‑1 (MQ=255) cGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGc < 1:1077121/47‑1 (MQ=255) cGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAcc < 1:3915947/71‑1 (MQ=255) cGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAcc > 1:1129861/1‑71 (MQ=255) gCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCa > 1:3572305/1‑44 (MQ=255) aGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTggg > 1:858424/1‑71 (MQ=255) gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc > 1:2471645/1‑47 (MQ=255) gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc > 1:275355/1‑47 (MQ=255) gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc < 1:2308493/56‑1 (MQ=255) gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGc < 1:4098780/71‑1 (MQ=255) tACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc < 1:3902485/55‑1 (MQ=255) tACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAc < 1:4224267/64‑1 (MQ=255) tACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAc < 1:4473442/64‑1 (MQ=255) tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGa < 1:586624/43‑1 (MQ=255) tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACg > 1:1134168/1‑52 (MQ=255) tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa < 1:3443943/63‑1 (MQ=255) tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa < 1:3506034/63‑1 (MQ=255) tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa < 1:370102/63‑1 (MQ=255) tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAc < 1:87738/64‑1 (MQ=255) tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCg < 1:2261958/71‑1 (MQ=255) aCCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACa > 1:2450768/1‑58 (MQ=255) ccATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGa < 1:5799/70‑1 (MQ=255) ccATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGAt > 1:3288982/1‑71 (MQ=255) ccATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCATACAGCAACCTGGGCGAt > 1:2337972/1‑71 (MQ=255) cATCTTCGGTCGTATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:2644012/71‑1 (MQ=255) cATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:3450098/71‑1 (MQ=255) cATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:1590987/71‑1 (MQ=255) cATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:2290848/71‑1 (MQ=255) cATCTTCGGACGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:4541826/71‑1 (MQ=255) aTCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTTGGCGATGt < 1:188391/71‑1 (MQ=255) tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCt < 1:2556038/51‑1 (MQ=255) tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa < 1:1039840/58‑1 (MQ=255) tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa < 1:4678286/58‑1 (MQ=255) tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTa > 1:1879790/1‑71 (MQ=255) tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTa > 1:707852/1‑71 (MQ=255) tCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTa < 1:262141/52‑1 (MQ=255) tCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:191344/69‑1 (MQ=255) tCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGAAACCTGGGCGATGTa > 1:737783/1‑71 (MQ=255) cTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTcgc < 1:4543674/42‑1 (MQ=255) ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGa > 1:3109557/1‑65 (MQ=255) ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:3034997/67‑1 (MQ=255) ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATg > 1:4252996/1‑71 (MQ=255) ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATg > 1:214247/1‑71 (MQ=255) ttCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACa > 1:1341354/1‑52 (MQ=255) tcggtcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATgg > 1:3898251/1‑71 (MQ=255) cggtcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATgg < 1:514963/70‑1 (MQ=255) cggtcggATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATgg > 1:961351/1‑70 (MQ=255) gtcggATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg < 1:1388323/63‑1 (MQ=255) gtcggATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCAt < 1:131639/71‑1 (MQ=255) tcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATc < 1:212964/71‑1 (MQ=255) tcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATc < 1:4617694/71‑1 (MQ=255) ggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGAc < 1:496171/37‑1 (MQ=255) ggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAg > 1:4121867/1‑71 (MQ=255) gatgatCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCt < 1:4269242/41‑1 (MQ=255) aTAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGt < 1:2403119/71‑1 (MQ=255) | ACGACGTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGT > minE/497777‑497915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |