| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 520,325 | T→C | 50.6% | V115V (GTT→GTC) | uvrB → | excinulease of nucleotide excision repair, DNA damage recognition component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 520,325 | 0 | T | C | 50.6% | 2.5 / 126.6 | 89 | V115V (GTT→GTC) | uvrB | excinulease of nucleotide excision repair, DNA damage recognition component |
| Reads supporting (aligned to +/- strand): ref base T (27/17); new base C (20/25); total (47/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTG > minE/520257‑520394 | tacGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTaa > 1:3456401/1‑71 (MQ=255)tacGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCaa > 1:3255151/1‑71 (MQ=255) acGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTa > 1:868403/1‑69 (MQ=255) tactaTCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAAc > 1:1122390/1‑66 (MQ=255) actaTCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACa > 1:2418585/1‑70 (MQ=255) ctaTCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACat > 1:1282879/1‑70 (MQ=255) ctaTCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACata < 1:3216365/71‑1 (MQ=255) aTCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACata > 1:1476435/1‑69 (MQ=255) tCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTa > 1:683768/1‑59 (MQ=255) tCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATAtt > 1:462256/1‑70 (MQ=255) tCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATAtt > 1:1527341/1‑70 (MQ=255) tCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTg < 1:1457773/71‑1 (MQ=255) aGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATagag > 1:3618655/1‑71 (MQ=255) aGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAg > 1:3460274/1‑71 (MQ=255) cgggAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCa < 1:2333944/69‑1 (MQ=255) cGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACatat < 1:2257339/64‑1 (MQ=255) cGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAg < 1:2597791/71‑1 (MQ=255) gAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGa > 1:2376170/1‑70 (MQ=255) gAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGa < 1:2859909/70‑1 (MQ=255) aaGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGa > 1:3497590/1‑64 (MQ=255) aaGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGc > 1:2813666/1‑66 (MQ=255) aGCCTATGTACCGAGTTCCGACACTTTCATT‑AGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGc < 1:2621926/70‑1 (MQ=255) gCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCg < 1:2735278/71‑1 (MQ=255) ccTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGt < 1:4440091/71‑1 (MQ=255) ccTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGt < 1:122000/71‑1 (MQ=255) tATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCa < 1:2148670/62‑1 (MQ=255) aTGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTg > 1:2031824/1‑71 (MQ=255) tGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGt < 1:3834743/71‑1 (MQ=255) tGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGt > 1:1608026/1‑71 (MQ=255) gTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTc > 1:69232/1‑71 (MQ=255) tACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTcc > 1:3619106/1‑71 (MQ=255) tACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTcc > 1:2372667/1‑71 (MQ=255) ccGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACat < 1:4619543/48‑1 (MQ=255) ccGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACat < 1:969612/48‑1 (MQ=255) ccGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACa < 1:3508780/47‑1 (MQ=255) ccGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACa < 1:2996136/47‑1 (MQ=255) ccGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGa > 1:657533/1‑58 (MQ=255) gAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGcca > 1:3480106/1‑71 (MQ=255) aGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGccac > 1:574630/1‑71 (MQ=255) aGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGccac > 1:236429/1‑71 (MQ=255) aGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGa > 1:918049/1‑55 (MQ=255) aGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATT‑AGCAGATGCGTTTGTCCGccac > 1:4643209/1‑70 (MQ=255) gTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGccac > 1:2151622/1‑70 (MQ=255) ttCCGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACat < 1:2709511/43‑1 (MQ=255) ccGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGa > 1:859296/1‑51 (MQ=255) ccGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGAt < 1:3898652/52‑1 (MQ=255) ccGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCaa < 1:2735664/70‑1 (MQ=255) ccGACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGa > 1:1612785/1‑51 (MQ=255) cGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCg < 1:817439/54‑1 (MQ=255) gACACTTTCATTGAGAAAGATGCCTCTGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGc < 1:2155513/71‑1 (MQ=255) gACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGc < 1:1385800/71‑1 (MQ=255) gACACTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCa < 1:2040536/47‑1 (MQ=255) acacTTTCATTGAGAAAGATGCCTCGGTTAACGAAc > 1:2294214/1‑36 (MQ=255) acacTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGtt > 1:3372001/1‑54 (MQ=255) acacTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAg > 1:2906310/1‑69 (MQ=255) acacTTTCATTGAGAAAGATGCCTCGGTCAACGAACatat > 1:2743867/1‑40 (MQ=255) acTTTCATTGAGAAAGATGCCTCGGTTAACGAACata > 1:586615/1‑37 (MQ=255) acTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGc < 1:4079841/49‑1 (MQ=255) acTTTCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGc < 1:1711754/68‑1 (MQ=255) tttCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGc > 1:3157624/1‑71 (MQ=255) ttCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGccac > 1:4481501/1‑59 (MQ=255) ttCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCt > 1:1092070/1‑71 (MQ=255) tCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTg > 1:4627428/1‑50 (MQ=255) tCATTGAGAAAGATGCCTCGGTTAAAGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGc > 1:4420312/1‑69 (MQ=255) tCATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGc < 1:3201597/69‑1 (MQ=255) cATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTg < 1:1390866/70‑1 (MQ=255) cATTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTg < 1:2438030/70‑1 (MQ=255) aTTGAGAAAGATGCCTCGGTCAACGAACATATTGAGc > 1:170037/1‑37 (MQ=255) aTTGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTgg < 1:3330365/70‑1 (MQ=255) ttGAGAAAGATGCCTCGGTCAACGAACATATTGAGCa < 1:2330563/37‑1 (MQ=255) ttGAGAAAGATGCCTCGGTCAACGAACATATTGAGCa < 1:2975209/37‑1 (MQ=255) ttGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTc > 1:3837382/1‑49 (MQ=255) ttGAGAAAGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGc < 1:1052846/61‑1 (MQ=255) gaAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAgcggc < 1:3319544/71‑1 (MQ=255) aaGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGt < 1:1653067/71‑1 (MQ=255) aaGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGt < 1:855430/71‑1 (MQ=255) aaGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGt < 1:2743123/71‑1 (MQ=255) aGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGt > 1:3388513/1‑70 (MQ=255) aGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTg < 1:2411406/71‑1 (MQ=255) aGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGt > 1:1186025/1‑70 (MQ=255) aGATGCCTCGGTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTg < 1:4418793/71‑1 (MQ=255) aTGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGAt > 1:3113287/1‑71 (MQ=255) tGCCTCGGTCAACGAACATATTGAGCAGATGCGttt > 1:2075655/1‑36 (MQ=255) tCGGTCAACGAACATATTGAGCAGATGCGTTTGTCAGc < 1:3372596/38‑1 (MQ=38) ggTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGAtgtggttg < 1:379797/71‑1 (MQ=255) ggTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGAtgtggt < 1:3282156/69‑1 (MQ=255) ggTCAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGAtgtggttg > 1:4375988/1‑71 (MQ=255) gTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGAtgtggtt > 1:1273239/1‑69 (MQ=255) gTTAACGAACATATTGAGCAGATGCGTTTGACCGCCACCAAAGCGATGCTGGAGCGGCGTGAtgtggttgt < 1:3573091/71‑1 (MQ=255) tAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGAtgtggttgtg < 1:2984061/70‑1 (MQ=255) | TACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTG > minE/520257‑520394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |