| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 549,018 | A→G | 54.1% | L321L (TTG→CTG) | ybiU ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 549,018 | 0 | A | G | 54.1% | 14.3 / 76.4 | 61 | L321L (TTG→CTG) | ybiU | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/16); new base G (20/13); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGTCGCAGTGCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGG > minE/548950‑549087 | gACGTCGCAGTGCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAAc < 1:2051902/70‑1 (MQ=255) aCGTCGCAGTGCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAg > 1:2963641/1‑71 (MQ=255) cAGTGCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTg < 1:4428448/67‑1 (MQ=255) cAGTGCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTg < 1:72888/67‑1 (MQ=255) aGTGCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGc < 1:3099809/71‑1 (MQ=255) gCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGAt > 1:2546829/1‑66 (MQ=255) ccaccaGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCAtt > 1:4548617/1‑71 (MQ=255) ccaGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGcc < 1:3251937/65‑1 (MQ=255) aGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTg > 1:2336052/1‑57 (MQ=255) aGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGctct < 1:3618087/71‑1 (MQ=255) aGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGctc < 1:1662600/70‑1 (MQ=255) aGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGAt > 1:1639560/1‑60 (MQ=255) gACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGctct > 1:3979050/1‑70 (MQ=255) gACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGctct > 1:4301616/1‑70 (MQ=255) aCGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCtcaatcaa < 1:780426/50‑1 (MQ=255) gacaGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGa > 1:3715178/4‑70 (MQ=255) cGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGAt < 1:3453219/71‑1 (MQ=255) ggAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCa > 1:2779805/1‑50 (MQ=255) ggAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCa > 1:2338947/1‑50 (MQ=255) ggAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCa > 1:4657249/1‑50 (MQ=255) tcCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGc > 1:4376556/1‑70 (MQ=255) tcCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACa > 1:2679914/1‑44 (MQ=255) tcCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAg > 1:2878889/1‑45 (MQ=255) ccGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTg < 1:6369/46‑1 (MQ=255) cGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCACTCAACAGTGGATGCCATTGCTCTGATACCGGCaa > 1:103142/1‑70 (MQ=255) cGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCTGCaa < 1:3926846/70‑1 (MQ=255) cGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCaa < 1:3908744/70‑1 (MQ=255) cGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAAt < 1:3323427/71‑1 (MQ=255) ggCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATa > 1:538463/1‑71 (MQ=255) ggCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATa > 1:1497343/1‑71 (MQ=255) gCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAAc < 1:2699521/39‑1 (MQ=255) ttCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGctct < 1:1027560/55‑1 (MQ=255) ttCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATActc > 1:792226/1‑71 (MQ=255) tCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTg > 1:1654630/1‑40 (MQ=255) cGAGTTTTGGAATGCTGGTTAACTCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATActct < 1:4308659/70‑1 (MQ=255) cGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATACTCtt > 1:4646006/1‑71 (MQ=255) gAGTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTg < 1:2234150/38‑1 (MQ=255) gTTTTGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGc < 1:365941/41‑1 (MQ=255) gTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTg < 1:1296299/36‑1 (MQ=255) gTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTg < 1:1663433/36‑1 (MQ=255) ttttGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCgg < 1:4119538/71‑1 (MQ=255) ttttGGAATGCTGGTTAACGCCTCAATCAACAGTgg < 1:758370/36‑1 (MQ=255) ttttGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGctct < 1:1360202/49‑1 (MQ=255) tttGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCaa > 1:432704/1‑59 (MQ=255) ttGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGa > 1:4404326/1‑49 (MQ=255) ttGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCggg > 1:4568234/1‑70 (MQ=255) ttGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATAACGGCAATACTCTTCCGGgc > 1:3063119/1‑71 (MQ=255) ttGGAATGCTGGTTAACGCCTCAATCAGCAGTGGATGCAATTg > 1:672459/1‑43 (MQ=38) aaTGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTa > 1:1857163/1‑71 (MQ=255) aaTGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTa > 1:354735/1‑71 (MQ=255) aTGCTGGTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAAt > 1:1867789/1‑54 (MQ=255) tGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGctct < 1:3187388/41‑1 (MQ=255) tGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGctct < 1:1614850/41‑1 (MQ=255) gTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGc < 1:1755786/45‑1 (MQ=255) gTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGca > 1:820945/1‑71 (MQ=255) gTTAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGca > 1:3496220/1‑71 (MQ=255) ttAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGca > 1:1230962/1‑70 (MQ=255) ttAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGca < 1:1748160/70‑1 (MQ=255) tAACGCCTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGca > 1:1716413/1‑69 (MQ=255) ctggCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAg < 1:4549320/68‑1 (MQ=255) aaCGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGcaca < 1:1064422/70‑1 (MQ=255) ccTCAATCAGCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCa < 1:3256435/71‑1 (MQ=255) tcaatcagCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCa < 1:2521749/69‑1 (MQ=255) tcaatcaaCAGTGGATGCCATTGCTCTGATACCGGCAATa > 1:1992175/1‑40 (MQ=255) aatcagCAGTGGATGCCATTGCTCTGATACCGGCAATa > 1:1136475/1‑38 (MQ=255) aatcagCAGTGGATGCCATTGCTCTGATACCGGCAATa > 1:3473075/1‑38 (MQ=255) aatcagCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTAcgc > 1:314913/1‑56 (MQ=255) aatcaaCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCAtcct > 1:954081/1‑71 (MQ=255) aatcaaCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCAtcct > 1:3476813/1‑71 (MQ=255) aatcaaCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCAtcct > 1:2107069/1‑71 (MQ=255) tcagCAGTGGATGCCATTGCTCTGATACCGGCAATAct < 1:3138040/38‑1 (MQ=255) tcagCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTcc < 1:2446068/43‑1 (MQ=255) tcagCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCAtcctcc > 1:2998272/1‑71 (MQ=255) caaCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGca < 1:1605607/57‑1 (MQ=255) aaCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGt > 1:3591276/1‑60 (MQ=255) aaCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCgg > 1:2436888/1‑71 (MQ=255) aaCAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCgg > 1:2258468/1‑71 (MQ=255) | GACGTCGCAGTGCCACCAGACGGAGTCTCCGGCTTCGAGTTTTGGAATGCTGGTTAACGCCTCAATCAACAGTGGATGCCATTGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGG > minE/548950‑549087 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |