| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 626,673 | G→A | 5.8% | D196N (GAT→AAT) | ycaQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 626,673 | 0 | G | A | 5.8% | 322.2 / 6.4 | 104 | D196N (GAT→AAT) | ycaQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (47/50); new base A (3/3); total (50/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
TGATGGTGATTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCC > minE/626605‑626742 | tgatggtgatTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGgat < 1:3819018/71‑1 (MQ=255)tgatggtgatTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGgat < 1:2225984/71‑1 (MQ=255)tgatggtgatTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGgat < 1:226071/71‑1 (MQ=255) tggtgatTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGgatgat < 1:369710/71‑1 (MQ=255) tggtgatTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGgatga > 1:1903008/1‑70 (MQ=255) gtgatTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGgatgat < 1:432842/69‑1 (MQ=255) gtgatTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGTatgatg < 1:10109/70‑1 (MQ=255) atTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAgc < 1:2758017/70‑1 (MQ=255) ttGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAgcgc < 1:491042/71‑1 (MQ=255) tGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAgcgc > 1:4401394/1‑70 (MQ=255) gAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGAtgatga < 1:3761772/65‑1 (MQ=255) aCGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGAtc < 1:1813816/71‑1 (MQ=255) aCGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGAtc < 1:2693692/71‑1 (MQ=255) gcAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGGGCGATCTCGtt < 1:2970791/71‑1 (MQ=255) gcAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGtt < 1:1237825/71‑1 (MQ=255) aaCTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTc > 1:3434084/1‑71 (MQ=255) cTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAg > 1:4095940/1‑55 (MQ=255) tCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCa > 1:1201661/1‑70 (MQ=255) ccAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGACGATGAGCGCGATCTCGTTTCGCaaa > 1:2732809/1‑71 (MQ=255) cAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:3051420/1‑70 (MQ=255) cAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa < 1:3449168/70‑1 (MQ=255) cAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa < 1:2431103/70‑1 (MQ=255) cAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa < 1:4242362/70‑1 (MQ=255) aGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:215403/1‑69 (MQ=255) aGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACa < 1:1973778/71‑1 (MQ=255) gcgcGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCa < 1:4363935/66‑1 (MQ=255) gcgcGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg < 1:3484304/71‑1 (MQ=255) cgcgTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg > 1:3616795/1‑70 (MQ=255) cgcgTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg < 1:179120/70‑1 (MQ=255) gcgTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg > 1:1905140/1‑69 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAgc > 1:3970652/1‑43 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAgc > 1:631150/1‑43 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAg > 1:2258857/1‑42 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACa > 1:4580616/1‑63 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:3886900/1‑70 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:675383/1‑70 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:980906/1‑70 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:1847141/1‑70 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:1954360/1‑70 (MQ=255) aTGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa > 1:1135134/1‑70 (MQ=255) aTGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa > 1:2542816/1‑70 (MQ=255) aTTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatc < 1:79980/71‑1 (MQ=255) tttAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatca < 1:2810462/71‑1 (MQ=255) tttAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatca > 1:3410706/1‑71 (MQ=255) ttAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat > 1:4423463/1‑71 (MQ=255) ttAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat > 1:3163914/1‑71 (MQ=255) tAACTCACCGTGTCATGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAg < 1:1840279/57‑1 (MQ=255) tAACTCACCGTGTCATGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAGAAGCAGAAatcat < 1:2784506/70‑1 (MQ=255) tAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat > 1:2351352/1‑70 (MQ=255) tAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat < 1:1778724/70‑1 (MQ=255) tAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat < 1:3209769/70‑1 (MQ=255) aaCCCACCGTGTCATGCCTGATTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcatc < 1:4342943/70‑1 (MQ=255) aaCCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcatc < 1:3883832/70‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCa > 1:3601949/1‑59 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCa > 1:3596656/1‑59 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:3312985/61‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:1172046/61‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:292902/61‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat < 1:2895403/67‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat < 1:489116/67‑1 (MQ=255) ccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat < 1:2715940/66‑1 (MQ=255) ccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:612569/1‑68 (MQ=255) ccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGc < 1:4080274/71‑1 (MQ=255) cGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTgg < 1:2159232/70‑1 (MQ=255) gtgtCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:2666423/55‑1 (MQ=255) tgtCATGCCTGACTGGGATGATGAGCGCGATCTCGtt < 1:1275705/37‑1 (MQ=255) gtCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGaaa > 1:3927254/1‑55 (MQ=255) gtCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:1573366/53‑1 (MQ=255) gtCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca < 1:3478437/61‑1 (MQ=255) gtCATGCCTGACTGGGATGATGAGAGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca < 1:565100/61‑1 (MQ=255) tCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat < 1:2743315/58‑1 (MQ=255) tCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:3147470/70‑1 (MQ=255) tCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAAc < 1:125329/71‑1 (MQ=255) tCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAAc < 1:4147106/71‑1 (MQ=255) cATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa > 1:2252527/1‑69 (MQ=255) cATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa > 1:1932846/1‑69 (MQ=255) cATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATa < 1:2185536/68‑1 (MQ=255) cATGCCTGACTGCGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:3913948/69‑1 (MQ=255) aTGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAg < 1:845258/43‑1 (MQ=255) aTGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAGAAGCAGAAATCATCATGCTGGATAACAg > 1:1512972/1‑71 (MQ=38) aTGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAGAAGCAGAAATCATCATGCTGGATAACAg > 1:4034851/1‑71 (MQ=38) aTGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaa > 1:2312185/1‑39 (MQ=255) aTGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaa > 1:4576149/1‑39 (MQ=255) tGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:970844/1‑39 (MQ=255) tGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:2118663/1‑57 (MQ=255) tGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:2296613/67‑1 (MQ=255) gCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:2730561/1‑38 (MQ=255) gCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:334367/1‑38 (MQ=255) ccTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAat < 1:717951/50‑1 (MQ=255) ccTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTg < 1:4281426/70‑1 (MQ=255) tGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:1989635/1‑53 (MQ=255) gACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGaaa > 1:1266206/1‑46 (MQ=255) gACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGcgcg > 1:2323947/1‑71 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGa < 1:4301114/36‑1 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:4581606/1‑50 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:546094/1‑50 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGt < 1:2044423/70‑1 (MQ=255) ggATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAg < 1:1830011/69‑1 (MQ=255) ggATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGcc < 1:3300234/71‑1 (MQ=255) ggATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGc < 1:230713/70‑1 (MQ=255) | TGATGGTGATTGAACGGCGCAACTTCCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCC > minE/626605‑626742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |